A5067744333- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Viral Infections and Immunology Research
- interferon and immune responses
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- CRISPR and Genetic Engineering
- Mollusks and Parasites Studies
- Marine Biology and Ecology Research
- HIV/AIDS Research and Interventions
- Paleontology and Stratigraphy of Fossils
- Virus-based gene therapy research
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
- Herpesvirus Infections and Treatments
- Respiratory viral infections research
- Plant Virus Research Studies
- Electrostatics and Colloid Interactions
- Geological Formations and Processes Exploration
Université de Strasbourg
2015-2024
Architecture et Réactivité de l'arN
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Institut de Biologie Moléculaire et Cellulaire
2006-2020
Universidad del Sureste
2020
Institut de Biologie Moléculaire des Plantes
1992-2014
Science Applications International Corporation (United States)
2007
Institut de Chimie
2007
Bridge University
2000
University of Cambridge
2000
The diploid genome of all retroviruses is made two homologous copies RNA intimately associated near their 5' end, in a region called the dimer linkage structure. Dimerization genomic thought to be important for crucial functions retroviral life cycle (reverse transcription, translation, encapsidation). Previous vitro studies mapped structure human immunodeficiency virus type 1 (HIV-1) downstream splice donor site, containing conserved purine tracts that were postulated mediate dimerization,...
RNA-RNA interactions govern a number of biological processes. Several RNAs, including natural sense and antisense interact by means two-step mechanism: recognition is mediated loop-loop complex, which then stabilized formation an extended intermolecular duplex. It was proposed that the same mechanism holds for dimerization genomic RNA human immunodeficiency virus type 1 (HIV-1), event thought to control crucial steps HIV-1 replication. However, whereas interaction between partially...
In all retroviruses, reverse transcription is primed by a tRNA whose 3' end 18 nucleotides are complementary to the so called viral primer binding site. Previous work showed that of HIV-1 RNA initiated tRNA(3Lys). Using variety chemical and enzymatic structural probes, we investigated interactions between its natural addition predictable contacts site tRNA(3Lys), specific interaction takes place an A-rich loop located upstream region anticodon This AAAA/Umcm5s2UUU loop-loop not observed when...
The genome of all retroviruses consists in two homologous RNA molecules associated near their 5' end i n a region called the dimer linkage structure.Dimerization genomic is thought to be important for several functions retroviral cycle such as encapsidation, reverse transcription, and translation.In human immunodeficiency virus type 1 (HIV-l), downstream splice donor site was initially postulated mediate dimerization.However, we recently showed that dimerization initiation located upstream...
In retroviruses, the genomic RNA is in form of a 60S-70S complex composed two identical genome-length molecules tightly associated through numerous interactions. A major interaction, called dimer linkage structure, has been found near 5' end and probably involved control translation, packaging, recombination during proviral DNA synthesis. Recently, small sequence corresponding to stem-loop structure located leader human immunodeficiency virus type 1 (HIV-1) was be required for initiation...
The retroviral genome consists of two identical RNA molecules joined close to their 5′ ends by the dimer linkage structure. Recent findings Indicated that dimerization and encapsidation are probably related events during virion assembly. We studied cation-induced HIV-1 results indicate all in vitro generated RNAs containing a 100 nucleotide domain downstream from splice site able dimerize. depends on concentration RNA, mono- multlvalent cations, size monovalent cation, temperature, pH. Up...
The influenza A virus genome consists of eight viral RNAs (vRNAs) that form ribonucleoproteins (vRNPs). Even though evidence supporting segment-specific packaging vRNAs is accumulating, the mechanism ensuring selective one copy each vRNA into particles remains largely unknown. We used electron tomography to show vRNPs emerge from a common 'transition zone' located underneath matrix layer at budding tip virions, where they appear be interconnected and often star-like structure. This zone...
Influenza A viruses cause annual influenza epidemics and occasional severe pandemics. Their genome is segmented into eight fragments, which offers evolutionary advantages but complicates genomic packaging. The existence of a selective packaging mechanism, in one copy each viral RNA specifically packaged virion, suspected, its molecular details remain unknown. Here, we identified direct intermolecular interaction between two segments an avian virus using vitro experiments. Using silent...
With the increasing interest of RNAs in regulating a range cell biological processes, very little is known about structure tissue culture cells. We focused on 5'-untranslated region human immunodeficiency virus type 1 RNA genome, highly conserved region, which contains structural domains that regulate key steps viral replication cycle. Up until now, information only came from vitro studies. Here, we developed chemical modification assays to test nucleotide accessibility directly infected...
Significance Genetic reassortment is one of the main mechanisms by which pandemic viruses emerge during influenza A coinfection, but little known about molecular affecting this process. Here, we studied genetic between a human and an avian strain, focusing on generation reassortant containing HA gene, have potential. We found that process was strongly biased, show packaging signals are crucial for suboptimal compatibility segment-specific two parental limits emergence viruses.
The genome of influenza A viruses (IAV) is split into eight viral RNAs (vRNAs) that are encapsidated as ribonucleoproteins. existence a segment-specific packaging mechanism well established, but the molecular basis this remains to be deciphered. Selective could mediated by direct interaction between vRNA regions, such interactions have never been demonstrated in virions. Recently, we showed vRNAs human H3N2 IAV form single network vitro involves regions known contain signals case H1N1...