A5100761126- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Transgenic Plants and Applications
- Immune Cell Function and Interaction
- Nematode management and characterization studies
- Toxin Mechanisms and Immunotoxins
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Insect symbiosis and bacterial influences
- Plant tissue culture and regeneration
- Bacterial Genetics and Biotechnology
- Cutaneous lymphoproliferative disorders research
- Lymphoma Diagnosis and Treatment
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Monoclonal and Polyclonal Antibodies Research
- Plant Reproductive Biology
- Plant Disease Management Techniques
- Salmonella and Campylobacter epidemiology
- Immunodeficiency and Autoimmune Disorders
- Forest Insect Ecology and Management
- Horticultural and Viticultural Research
Centre National de la Recherche Scientifique
2011-2024
Université de Strasbourg
2011-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2024
Santé et Agroécologie du Vignoble
2021
Institut de Biologie Moléculaire des Plantes
1999-2020
Institut Français de la Vigne et du Vin
2015
Inserm
1989-2014
Sorbonne Université
1993-2014
Université Paris Cité
2014
Institut du Cerveau
2014
Plant UDP-Glc:phenylpropanoid glucosyltransferases (UGTs) catalyze the transfer of Glc from UDP-Glc to numerous substrates and regulate activity compounds that play important roles in plant defense against pathogens. We previously characterized two tobacco salicylic acid– pathogen-inducible UGTs (TOGTs) act very efficiently on hydroxycoumarin scopoletin hydroxycinnamic acids. To identify physiological these defense, we generated TOGT-depleted plants by antisense expression. After inoculation...
Thirty-two clinical isolates of Shiga-like toxin (SLT)-producing Escherichia coli associated with single cases or outbreaks bloody diarrhea, hemorrhagic colitis, the hemolytic uremic syndrome, edema disease swine were examined for multiple copies genes belonging to slt-I slt-II families. Five 19 strains that known produce SLT-II hybridize slt-II-specific probes by colony blot found Southern hybridization contain two related slt-II. The toxins closely cloned from one isolates, O157:H- strain...
Plasmodesmata (PD) are essential but poorly understood structures in plant cell walls that provide symplastic continuity and intercellular communication pathways between adjacent cells thus play fundamental roles development pathogenesis. Viruses encode movement proteins (MPs) modify these tightly regulated pores to facilitate their spread from cell. The most striking of modifications is observed for groups viruses whose MPs form tubules assemble PDs through which virions transported...
Sézary syndrome is a cutaneous T cell lymphoma characterized by infiltration of the skin CD4+ cells. These cells generally respond poorly to mitogens and activators. We have studied action IL1 IL4, IL6, IL7 on proliferation from 12 patients. With exception IL2 IL7, cytokines had no proliferative effect these Whereas only low capacity (two- threefold increase) peripheral blood mononuclear cells, recombinant constantly induced very significant (3-40-fold response, was used successfully...
Cell-to-cell movement of plant viruses occurs via plasmodesmata (PD), organelles that evolved to facilitate intercellular communications. Viral proteins (MP) modify PD allow passage the virus particles or nucleoproteins. This several distinct mechanisms one which is MP-dependent formation tubules traverse and provide a conduit for virion translocation. The MP tubule-forming including Grapevine fanleaf (GFLV) recruit receptors called Plasmodesmata Located Proteins (PDLP) mediate tubule...
Infection by Grapevine fanleaf nepovirus (GFLV), a bipartite RNA virus of positive polarity belonging to the Comoviridae family, causes extensive cytopathic modifications host endomembrane system that eventually culminate in formation perinuclear "viral compartment." We identified immunoconfocal microscopy this compartment as site replication since it contained RNA1-encoded proteins necessary for replication, newly synthesized viral RNA, and double-stranded replicative forms. In addition,...
Characteristically, enterohemorrhagic Escherichia coli (EHEC) strains produce Shiga-like toxin type I (SLT-I), SLT-II, or both of these immunologically distinct cytotoxins. No antigenic receptor-binding variants SLT-I have been identified, but a number SLT-II-related toxins described. Because EHEC O91:H21 strain B2F1, which produces two toxins, is exquisitely virulent in an orally infected, streptomycin-treated mouse model (oral 50% lethal dose [LD50], < 10 organisms), we asked whether...
Summary Since their discovery, single‐domain antigen‐binding fragments of camelid‐derived heavy‐chain‐only antibodies, also known as nanobodies (Nbs), have proven to be outstanding interest therapeutics against human diseases and pathogens including viruses, but use phytopathogens remains limited. Many plant viruses Grapevine fanleaf virus ( GFLV ), a nematode‐transmitted icosahedral causal agent degenerative disease, worldwide distribution huge burden on crop yields representing billions US...
The 5'-terminal cistron of beet necrotic yellow vein furovirus RNA 2 encodes the 21 kDa major viral coat protein and terminates with an amber stop codon which can undergo suppression to give rise a 75 readthrough (RT) referred as P75. P75 is minor component virions 54 RT domain following sequence important both for virus assembly transmission by fungal vector Polymyxa betae. To better define regions involved in these two steps, transcripts encoding different in-frame deletion mutants were...
We analyzed Escherichia coli O157:H7 isolates from stool samples of five patients who had bloody diarrhea and were infected during a large food-borne outbreak hemorrhagic colitis in Washington state. The assessed for Shiga-like toxin profile, adherence plasmid traits, mouse virulence, capsule, enterohemolysin production. profiles the indistinguishable each other similar to that E. strain EDL933, an organism responsible hamburger-associated food poisoning episode 1982.
Abstract Thioredoxins (Trxs) constitute a family of small proteins in plants. This has been extensively characterized Arabidopsis (Arabidopsis thaliana), which contains six different Trx types: f, m, x, and y chloroplasts, o mitochondria, h mainly cytosol. A detailed study this the model legume Medicago truncatula, realized here, established existence two isoforms that do not belong to any types previously described. As no possible orthologs were further found either rice (Oryza sativa) or...
Salmonella typhimurium ST39 exhibits reduced virulence in mice and decreased survival mouse macrophages compared with the parent strain SL3201. Strain is nonmotile, carries an indeterminate deletion near flgB operon, defective mviS (mouse Salmonella) locus. In flagellum-defective strains, flgM gene product of S. negatively regulates flagellar genes by inhibiting activity FliA, flagellin-specific sigma factor. this study, wild-type LT2 was found to complement defect for enhanced macrophages....
RNA 2 of the multipartite genome beet necrotic yellow vein virus carries cistron for 21-kDa viral coat protein at its 5' extremity. The amber termination codon undergoes suppression approximately 10% time so that translation continues into an adjacent 54-kDa open reading frame, yielding a 75-kDa readthrough protein. roles and in infection were investigated with biologically active transcripts 2. Much transcript could be deleted without interfering replication local lesion formation on...
Many animal and plant viruses rely on vectors for their transmission from host to host. Grapevine fanleaf virus (GFLV), a picorna-like plants, is transmitted specifically by the ectoparasitic nematode Xiphinema index. The icosahedral capsid of GFLV, which consists 60 identical coat protein subunits (CP), carries determinants this specificity. Here, we provide novel insight into GFLV nematodes through comparative structural functional analysis two variants. We isolated mutant strain (GFLV-TD)...
Supernatants from Plasmodium falciparum continuous cultures exhibited mitogenic activity against human blood lymphocytes unsensitized donors. This effect, which was not observed with supernatants control grown in the absence of parasites, dependent upon (i) concentration supernatant added to lymphocyte and (ii) parasite P. cultures. T cells were predominant target this activity. We similar response malaria-sensitized individuals culture material. also detected a parasite-infected...
Factors involved in symptom expression of viruses from the genus Nepovirus family Secoviridae such as grapevine fanleaf virus (GFLV) are poorly characterized. To identify determinants encoded by GFLV, infectious cDNA clones RNA1 and RNA2 strain GHu were developed used alongside existing F13 a reverse genetics approach. In vitro transcripts homologous combinations induced systemic infection Nicotiana benthamiana clevelandii with identical phenotypes to WT strains, i.e. vein clearing chlorotic...