A5035959736- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
- Plant Parasitism and Resistance
- Animal Disease Management and Epidemiology
- Insect Resistance and Genetics
- Plant and Fungal Interactions Research
- Viral Infections and Vectors
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Insect and Arachnid Ecology and Behavior
- Viral Infectious Diseases and Gene Expression in Insects
- Entomopathogenic Microorganisms in Pest Control
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- Plant Disease Resistance and Genetics
- Viral Infections and Immunology Research
- Plant and animal studies
- Insect and Pesticide Research
- CRISPR and Genetic Engineering
- Evolution and Genetic Dynamics
- Toxin Mechanisms and Immunotoxins
- Insect Utilization and Effects
- Monoclonal and Polyclonal Antibodies Research
NeuroDiderot
2018-2024
École Nationale Vétérinaire d'Alfort
2018-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2018-2024
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2004-2022
Centre Occitanie-Montpellier
2022
Institut de Biologie Moléculaire des Plantes
2013-2021
Université de Strasbourg
2014-2021
Centre National de la Recherche Scientifique
2013-2021
Biologie et Génétique des Interactions Plante-Parasite
2005-2019
Plant viruses transmitted by insects cause tremendous losses in most important crops around the world. The identification of receptors plant within their insect vectors is a key challenge to understanding mechanisms transmission and offers an avenue for future alternative control strategies limit viral spread. We here report two cuticular proteins aphid mouthparts, we provide experimental support role one them noncirculative virus. These proteins, named Stylin-01 Stylin-02, belong RR-1...
Recombination, complementation and competition profoundly influence virus evolution epidemiology. Since viruses are intracellular parasites, the basic parameter determining potential for such interactions is multiplicity of cellular infection (cellular MOI), i.e. number viral genome units that effectively infect a cell. The MOI values prevail in host organisms have rarely been investigated, whether they remain constant or change widely during invasion totally unknown. Here, we fill this...
The effective size of populations (Ne) determines whether selection or genetic drift is the predominant force shaping their structure and evolution. Despite high mutation rate rapid evolution, this parameter poorly documented experimentally in viruses, particularly plant viruses. All available studies, however, have demonstrated existence huge within-host demographic fluctuations, drastically reducing Ne upon systemic invasion different organs tissues. Notably, extreme bottlenecks been...
Summary Since their discovery, single‐domain antigen‐binding fragments of camelid‐derived heavy‐chain‐only antibodies, also known as nanobodies (Nbs), have proven to be outstanding interest therapeutics against human diseases and pathogens including viruses, but use phytopathogens remains limited. Many plant viruses Grapevine fanleaf virus ( GFLV ), a nematode‐transmitted icosahedral causal agent degenerative disease, worldwide distribution huge burden on crop yields representing billions US...
Aphid-transmitted plant viruses are a threat for major crops causing massive economic loss worldwide. Members in the Luteoviridae family transmitted by aphids circulative and non-replicative mode. Virions acquired when ingesting sap from infected plants transported through gut accessory salivary gland cells transcytosis mechanism relying on virus-specific receptors largely unknown. Once released into canal, virions inoculated to plants, together with saliva, during subsequent feeding. In...
Cucurbit aphid-borne yellows virus (CABYV) is a polerovirus (Luteoviridae family) with capsid composed of the major coat protein and minor component referred to as readthrough (RT). Two forms RT were reported: full-length 74 kDa detected in infected plants truncated form 55 (RT*) incorporated into virions. Both CABYV-infected plants. To clarify specific roles each viral cycle, we generated by deletion mutant able synthesize only RT* which particle. This was unable move systemically from...
Double-stranded RNA (dsRNA) plays essential functions in many biological processes, including the activation of innate immune responses and interference. dsRNA also represents genetic entity some viruses is a hallmark infections by positive-sense single-stranded viruses. Methods for detecting rely essentially on immunological approaches their use often limited to vitro applications, although recent developments have allowed visualization vivo. Here, we report sensitive rapid detection long...
Abstract Plant RNA viruses form organized membrane-bound replication complexes to replicate their genomes. This process requires virus- and host-encoded proteins leads the production of double-stranded (dsRNA) intermediates. Here, we describe use Arabidopsis thaliana expressing GFP-tagged dsRNA-binding protein (B2:GFP) pull down dsRNA associated in planta upon infection with Tobacco rattle virus (TRV). Mass spectrometry analysis dsRNA-B2:GFP-bound from infected plants revealed presence viral...
Viral genomic RNA of the Turnip yellows virus (TuYV; genus Polerovirus ; family Luteoviridae ) is protected in virions formed by major capsid protein (CP) and minor component, readthrough (RT*) protein. Long-distance transport, used commonly viruses to systemically infect host plants, occurs phloem sieve elements two viral forms transport have been described: ribonucleoprotein (RNP) complexes. With regard poleroviruses, always presumed be long-distance form, but potential role RNP complexes...
With the increasing availability of aphid genomic data, it is necessary to develop robust functional validation methods evaluate role specific genes. This work represents first study in which five different techniques, all based on RNA interference and oral acquisition double-stranded (dsRNA), were developed silence two genes, ALY Eph, potentially involved polerovirus transmission by aphids. Efficient silencing only Eph transcripts, are less abundant than those ALY, could be achieved feeding...
Bioinformatic analyses have predicted that orbiviruses encode an additional, small non-structural protein (NS5) from a secondary open reading frame on genome segment 10. However, this has not previously been detected in infected mammalian or insect cells. NS5-specific antibodies were generated mice and used to identify NS5 synthesised orbivirus-infected BSR cells transfected with expression plasmids. Confocal microscopy shows although accumulates the nucleus, particularly nucleolus, which...
Abstract Aphids are the primary vectors of plant viruses. Transmission can occur via attachment to cuticle lining insect (non‐circulative transmission) or after internalization in cells with without replication (circulative transmission). In this paper, we have focused on circulative and non‐propagative mode during which virions enter cell following receptor‐mediated endocytosis, transported across vesicles released by exocytosis replicating. The correct uptake, transport delivery cargo...
Vaccinia virus (Orthopoxvirus) F17 protein is a major virion structural phosphoprotein having molecular weight of 11 kDa. Recently, it was shown that synthesised in infected cells interacts with mTOR subunits to evade cell immunity and stimulate late viral synthesis. Several years back, we purified an kDa inhibited synthesis reticulocyte lysate from virions, possesses all physico-chemical properties protein. To investigate this discrepancy, used defective vaccinia particles devoid the...
Bluetongue is an economically important disease of domesticated and wild ruminants caused by bluetongue virus (BTV). There are at least 36 different serotypes BTV (the identity which determined its outer-capsid protein VP2), most transmitted Culicoides biting midges. IFNAR(-/-) mice immunised with plant-expressed VP2 (rVP2) -1, -4 or -8, the smaller rVP5 BTV-10, mock-immunised PBS, were subsequently challenged virulent strains BTV-4 BTV-8, attenuated clone BTV-1 (BTV-1RGC7). The that had...
Integration of non-retroviral sequences in the genome different organisms has been observed and, some cases, a relationship these integrations with immunity established. The green peach aphid, Myzus persicae (clone G006), was screened for densovirus-like sequence (DLS) integrations. A total 21 DLSs localized on 10 scaffolds were retrieved that mostly shared identity two aphid-infecting viruses, densovirus (MpDNV) and Dysaphis plantaginea (DplDNV). In uninterrupted potential ORFs...
The phloem-limited poleroviruses infect Arabidopsis thaliana without causing noticeable disease symptoms. In order to facilitate visual infection identification, we developed virus-induced gene silencing (VIGS) vectors derived from Turnip yellows virus (TuYV). Short sequences the host AtCHLI1 required for chlorophyll biosynthesis (42 nucleotides in sense or antisense orientation as an inverted-repeat (IR), 81 nucleotide fragment) were inserted into 3' non-coding region of TuYV genome screen...
Culicoides biting midges (Diptera: Ceratopogonidae) transmit arboviruses of veterinary or medical importance, including bluetongue virus (BTV) and Schmallenberg virus, as well causing severe irritation to livestock humans. Arthropod cell lines are essential laboratory research tools for the isolation propagation vector-borne pathogens investigation host-vector-pathogen interactions. Here we report establishment two continuous lines, CNE/LULS44 CNE/LULS47, from embryos nubeculosus, a midge...
Statin derivatives can inhibit the replication of a range viruses, including hepatitis C virus (HCV, Hepacivirus), dengue (Flavivirus), African swine fever (Asfarviridae) and poliovirus (Picornaviridae). We assess antiviral effect fluvastatin in cells infected with orbiviruses (bluetongue (BTV) Great Island (GIV)). The synthesis orbivirus outer-capsid protein VP2 (detected by confocal immunofluorescence imaging) was used to levels replication, showing reduction fluvastatin-treated cells. A...
To avoid the activation of plant defenses and ensure sustained feeding, aphids are assumed to use their mouthparts deliver effectors into cells. A recent study has shown that detected near feeding sites differentially distributed in tissues. However, precise process effector delivery specific compartments is unknown. The acrostyle, a cuticular organ located at tip maxillary stylets transiently binds viruses via its stylin proteins, may participate this process. Here, we demonstrate Mp10,...
Bluetongue virus serotypes 1 to 24 are transmitted primarily by infected Culicoides midges, in which they also replicate. However, "atypical" BTV (BTV-25, -26, -27 and -28) have recently been identified that do not infect replicate adult Culicoides, or a derived cell line (KC cells). These atypical viruses horizontally direct contact between susceptible hosts (primarily small ruminants) causing only mild clinical signs, although the exact transmission mechanisms involved yet be determined....