A5091482962- Plant Virus Research Studies
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- Plant tissue culture and regeneration
- Viral gastroenteritis research and epidemiology
- Legume Nitrogen Fixing Symbiosis
- Plant and Fungal Interactions Research
- CRISPR and Genetic Engineering
- Agricultural pest management studies
- Advanced biosensing and bioanalysis techniques
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Toxin Mechanisms and Immunotoxins
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Disease Management and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Plant Disease Resistance and Genetics
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Biosensors and Analytical Detection
- Hepatitis Viruses Studies and Epidemiology
Norwich Research Park
2016-2025
John Innes Centre
2016-2025
Norwich University
1982-2019
Thammasat University
2019
George Mason University
2015
Scripps Research Institute
1997-2015
United States Naval Research Laboratory
2015
Naval Research Laboratory Center for Bio/Molecular Science and Engineering
2015
Bentham Science Publishers (China)
2013
Bentham Science Publishers (United Arab Emirates)
2013
Agro-infiltration of leaf tissue with binary vectors harbouring a sequence interest is rapid method expressing proteins in plants. It has recently been shown that flanking the to be expressed modified 5'-untranslated region (UTR) and 3'-UTR from Cowpea mosaic virus (CPMV) RNA-2 (CPMV-HT) within vector pBINPLUS greatly enhances level expression can achieved [Sainsbury, F. Lomonossoff, G.P. (2008)Plant Physiol. 148, 1212-1218]. To exploit this finding, series small tailored for transient...
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us complete viral sequence and study its variability within population. The positive strand coding starts 69 nucleotides from 5' end with reading frame for protein Mr 125,941 terminates UAG. Readthrough this terminator would give rise 183,253. Overlapping terminal five codons readthrough is second 29,987. This gene two before...
The substrate specificity of micrococcal nuclease (EC 3.1.4.7.) has been studied. enzyme recognises features nucleotide composition, sequence and tertiary structure DNA. Kinetic analysis indicates that the rate cleavage is 30 times greater at 5' side A or T than G C. Digestion end-labelled linear DNA molecules known revealed only a limited number sites are cut, generating highly specific pattern fragments. frequency each site determined it may reflect poor base overlap in T-A 3' stack as...
Abstract Plant-based overexpression of heterologous proteins has attracted much interest and development in recent years. To date, the most efficient vectors have been based on RNA virus-derived replicons. A system a disabled version cowpea mosaic virus RNA-2 developed, which overcomes limitations insert size introduces biocontainment. This involves positioning gene between 5′ leader sequence 3′ untranslated region (UTR) RNA-2, thereby emulating presumably stable mRNA for translation. Thus...
Beneficial microbes in the microbiome of plant roots improve health. Induced systemic resistance (ISR) emerged as an important mechanism by which selected growth–promoting bacteria and fungi rhizosphere prime whole body for ...Read More
Nicotiana tabacum cv. Xanthi nn plants were transformed with nucleotides 3472-4916 of tobacco mosaic virus (TMV) strain U1. This sequence contains all but the three 3 terminal TMV 54-kDa gene, which encodes a putative component replicase complex. These resistant to infection when challenged either U1 virions or RNA at concentrations up 500 micrograms/ml 300 micrograms/ml, respectively, highest tested. Resistance was also exhibited inoculated 100 closely related mutant YSI/1 not shown in same...
The nucleotide sequence of the RNA tobacco vein mottling virus, a member potyvirus group, was determined. found to be 9471 residues in length, excluding 3′-terminal poly(A) tail. first three AUG codons from 5′-terminus were followed by in-frame termination codons. fourth, at position 206, beginning an open reading frame 9015 which could encode polyprotein 340 kDa. No other long frames present or its complement. This AGGCCAUG, is similar consensus initiation shared most eukaryotic mRNAs....
Nearly 20 percent of the packaged RNA in bean-pod mottle virus (BPMV) binds to capsid interior a symmetric fashion and is clearly visible electron density map. The displaying icosahedral symmetry single-stranded with well-defined polarity stereochemical properties. Interactions protein are dominated by nonbonding forces few specific contacts. tertiary quaternary structures BPMV proteins similar those observed animal picornaviruses, supporting close relation between plant comoviruses...
Abstract Serine carboxypeptidase-like (SCPL) proteins have recently emerged as a new group of plant acyltransferases. These enzymes share homology with peptidases but lack protease activity and instead are able to acylate natural products. Several SCPL acyltransferases been characterized date from dicots, including an enzyme required for the synthesis glucose polyesters that may contribute insect resistance in wild tomato (Solanum pennellii) sinapate esters associated UV protection...
Members of the cytochromes P450 superfamily (P450s) catalyze a huge variety oxidation reactions in microbes and higher organisms. Most families are highly divergent, but contrast cytochrome 14α-sterol demethylase (CYP51) family is one most ancient conserved, catalyzing sterol 14α-demethylase required for essential synthesis across fungal, animal, plant kingdoms. Oats (Avena spp.) produce antimicrobial compounds, avenacins, that provide protection against disease. Avenacins synthesized from...
The plant virus, Cowpea mosaic virus (CPMV), is developed as a carrier of the chemotherapeutic drug doxorubicin (DOX). CPMV-DOX conjugate, in which eighty DOX molecules are covalently bound to external surface carboxylates viral nanoparticle (VNP), shows greater cytotoxicity than free toward HeLa cells when administered at low dosage. At higher concentrations, time-delayed. CPMV conjugate targeted endolysosomal compartment cells, proteinaceous degraded and released. This study first...
Summary Plant expression systems based on nonreplicating virus‐based vectors can be used for the simultaneous of multiple genes within same cell. They therefore have great potential production heteromultimeric protein complexes. This work describes efficient plant‐based and assembly B luetongue virus‐like particles ( VLP s), requiring four distinct proteins in varying amounts. Such to serve as a safe effective vaccine against virus BTV ), which causes high mortality rates ruminants thus has...
Recent developments in transient expression methods have enabled the efficient delivery and of multiple genes within same plant cell over a timescale days. In some cases, vectors deployed can be fine-tuned to allow differential various genes. This has opened way deployment for such applications as production macromolecular complexes analysis manipulation metabolic pathways. The ability observe effect gene matter days means that is becoming method choice many plant-based synthetic biology...
The core protein of the hepatitis B virus, HBcAg, assembles into highly immunogenic virus-like particles (HBc VLPs) when expressed in a variety heterologous systems. Specifically, major insertion region (MIR) on HBcAg allows foreign sequences, which are then exposed tips surface spike structures outside assembled particle. Here, we present novel strategy aids display whole proteins HBc particles. This strategy, named tandem core, is based production dimer as single polypeptide chain by...
Poliovirus (PV) is the causative agent of poliomyelitis, a crippling human disease known since antiquity. PV occurs in two distinct antigenic forms, D and C, which only form elicits robust neutralizing response. Developing synthetically produced stabilized virus-like particle (sVLP)-based vaccine with antigenicity, without drawbacks current vaccines, will be major step towards final eradication poliovirus. Such sVLP would retain native conformation repetitive structure original virus...
Abstract Operon-like gene clusters are an emerging phenomenon in the field of plant natural products. The genes encoding some best-characterized secondary metabolite biosynthetic pathways scattered across genomes. However, increasing number synthesis diverse products have recently been reported These arisen through neo-functionalization and relocation existing within genome, not by horizontal transfer from microbes. reasons for clustering yet clear, although this form organization is likely...