A5069230408- Carbohydrate Chemistry and Synthesis
- Cassava research and cyanide
- Microbial Metabolites in Food Biotechnology
- HIV/AIDS drug development and treatment
- Enzyme Production and Characterization
- Food composition and properties
- Synthesis and Characterization of Heterocyclic Compounds
- Glycosylation and Glycoproteins Research
- Isotope Analysis in Ecology
- Polysaccharides and Plant Cell Walls
- Plant biochemistry and biosynthesis
- Biochemical and biochemical processes
- HIV Research and Treatment
- Biological Control of Invasive Species
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- DNA and Nucleic Acid Chemistry
- Cancer therapeutics and mechanisms
- Phytase and its Applications
- Biochemical and Molecular Research
- Microbial Natural Products and Biosynthesis
- Plant Molecular Biology Research
- Synthesis and Biological Evaluation
- Pneumocystis jirovecii pneumonia detection and treatment
- Enzyme Catalysis and Immobilization
University of Copenhagen
2016-2025
Laboratoire de Biochimie
2001-2017
Planta
2016-2017
The Velux Foundations
2011-2013
Centre National de la Recherche Scientifique
2004
Centre de Recherches sur les Macromolécules Végétales
2004
Technical University of Denmark
2003
Odense University Hospital
1987-1995
Statens Serum Institut
1992
Odense Municipality
1992
ABSTRACT Vanillin is one of the world's most important flavor compounds, with a global market 180 million dollars. Natural vanillin derived from cured seed pods vanilla orchid ( Vanilla planifolia ), but synthesized petrochemicals or wood pulp lignins. We have established true de novo biosynthetic pathway for production glucose in Schizosaccharomyces pombe , also known as fission yeast African beer yeast, well baker's Saccharomyces cerevisiae . Productivities were 65 and 45 mg/liter, after...
Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling intermediates and prevent undesired metabolic cross-talk while maintaining flexibility. Here we report isolation dynamic metabolon that catalyzes formation cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The was reconstituted liposomes, which demonstrated importance membrane surface charge presence...
Abstract Vanillin is a popular and valuable flavour compound. It the key constituent of natural vanilla obtained from cured pods. Here we show that single hydratase/lyase type enzyme designated vanillin synthase ( Vp VAN) catalyses direct conversion ferulic acid its glucoside into glucoside, respectively. The shows high sequence similarity to cysteine proteinases specific substitution pattern at aromatic ring does not metabolize caffeic p -coumaric as demonstrated by coupled...
Phenolic cross-links and inter-unit linkages result from the oxidative coupling of hydroxycinnamates leading to lignin assembly cross-linking with cell wall polysaccharides extensin proteins.
Cyanogenic glucosides are amino acid-derived defence compounds found in a large number of vascular plants. Their hydrolysis by specific β-glucosidases following tissue damage results the release hydrogen cyanide. The cyanogenesis deficient1 (cyd1) mutant Lotus japonicus carries partial deletion CYP79D3 gene, which encodes cytochrome P450 enzyme that is responsible for first step cyanogenic glucoside biosynthesis. genomic region surrounding contains genes encoding CYP736A2 protein and...
Members of the cytochromes P450 superfamily (P450s) catalyze a huge variety oxidation reactions in microbes and higher organisms. Most families are highly divergent, but contrast cytochrome 14α-sterol demethylase (CYP51) family is one most ancient conserved, catalyzing sterol 14α-demethylase required for essential synthesis across fungal, animal, plant kingdoms. Oats (Avena spp.) produce antimicrobial compounds, avenacins, that provide protection against disease. Avenacins synthesized from...
Cyanogenic glycosides are phytoanticipins involved in plant defence against herbivores by virtue of their ability to release toxic hydrogen cyanide (HCN) upon tissue disruption. In addition, endogenous turnover cyanogenic without the liberation HCN may offer plants an important source reduced nitrogen at specific developmental stages. To investigate presence putative products glycosides, comparative metabolic profiling using LC–MS/MS and high resolution MS (HR–MS) complemented ion-mobility...
Forskolin is a unique structurally complex labdane-type diterpenoid used in the treatment of glaucoma and heart failure based on its activity as cyclic AMP booster. Commercial production forskolin relies exclusively extraction from only known natural source, plant Coleus forskohlii, which accumulates root cork. Here, we report discovery five cytochrome P450s two acetyltransferases catalyze cascade reactions converting precursor 13R-manoyl oxide into diverse array additional diterpenoids. A...
Despite vast diversity in metabolites and the matching substrate specificity of their transporters, little is known about how evolution transporter specificities linked to emergence substrates via biosynthetic pathways. Transporter towards recently evolved glucosinolates characteristic Brassicales shown evolve prior glucosinolate biosynthesis. Furthermore, we show that transporters belonging ubiquitous NRT1/PTR FAMILY (NPF) likely from ancestral cyanogenic glucosides found across more than...
Abstract Synthetic biology efforts for the production of valuable chemicals are frequently hindered by structure and regulation native metabolic pathways chassis. This is particularly evident in case monoterpenoid Saccharomyces cerevisiae , where canonical terpene precursor geranyl diphosphate tightly coupled to biosynthesis isoprenoid compounds essential yeast viability. Here, we establish a synthetic orthogonal pathway based on an alternative precursor, neryl diphosphate. We identify...
Abstract Two homologous plant-specific Arabidopsis thaliana genes, RGXT1 and RGXT2, belong to a new family of glycosyltransferases (CAZy GT-family-77) encode cell wall (1,3)-α-d-xylosyltransferases. The deduced amino acid sequences contain single transmembrane domains near the N terminus, indicative type II membrane protein structure. Soluble secreted forms corresponding proteins expressed in insect cells showed xylosyltransferase activity, transferring d-xylose from UDP-α-d-xylose l-fucose....
Cassava (Manihot esculenta) is a eudicotyledonous plant that produces the valine- and isoleucine-derived cyanogenic glucosides linamarin lotaustralin with corresponding oximes cyanohydrins as key intermediates. CYP79 enzymes catalyzing amino acid-to-oxime conversion in glucoside biosynthesis are known from several plants including cassava. The enzyme system converting oxime into cyanohydrin has previously only been identified monocotyledonous great millet (Sorghum bicolor). Using this...
The lack of robust, high-throughput, and sensitive analytical strategies that can conclusively map the structure glycans has significantly hampered progress in fundamental applied aspects glycoscience. Resolution anomeric α/β glycan linkage within oligosaccharides remains a particular challenge. Here, we show "memory" configuration is retained following gas-phase glycosidic bond fragmentation during tandem mass spectrometry (MS2). These findings allow for integration MS2 with ion mobility...
Abstract Genomic gene clusters for the biosynthesis of chemical defence compounds are increasingly identified in plant genomes. We previously reported independent evolution biosynthetic cyanogenic glucoside three lineages. Here we report that cluster dhurrin Sorghum bicolor additionally contains a gene, SbMATE2 , encoding transporter multidrug and toxic compound extrusion (MATE) family, which is co-expressed with genes. The predicted localisation to vacuolar membrane was demonstrated...
Almond and sweet cherry are two economically important species of the Prunus genus. They both produce cyanogenic glucosides prunasin amygdalin. As part a two-component defense system, amygdalin release toxic hydrogen cyanide upon cell disruption. In this study, we investigated potential role some its derivatives in endodormancy these species. The content endogenous turnover products course flower development/opening was examined five almond cultivars – differing from very early to extra-late...
Cyanogenic glucosides are nitrogen-containing specialized metabolites that provide chemical defense against herbivores and pathogens via the release of toxic hydrogen cyanide. It has been suggested cyanogenic also a store nitrogen can be remobilized for general metabolism previously unknown pathway. Here we reveal recycling pathway glucoside dhurrin in sorghum (Sorghum bicolor) avoids cyanide formation. As demonstrated vitro, proceeds spontaneous formation dhurrin-derived glutathione...
Cytochrome P450s (P450s) are key enzymes in the synthesis of bioactive natural products plants. Efforts to harness these for vitro and whole-cell production have been hampered by difficulties expressing them heterologously their active form, requirement NADPH as a source reducing power. We recently demonstrated targeting insertion plant into photosynthetic membrane photosynthesis-driven, NADPH-independent P450 catalytic activity mediated electron carrier protein ferredoxin. Here, we report...
Phytocannabinoids are a group of plant-derived metabolites that display wide range psychoactive as well health-promoting effects. The production pharmaceutically relevant cannabinoids relies on extraction and purification from cannabis (Cannabis sativa) plants yielding the major constituents, Δ9-tetrahydrocannabinol cannabidiol. Heterologous biosynthesis in Nicotiana benthamiana or Saccharomyces cerevisiae may provide cost-efficient rapid future platforms to acquire pure high quantities both...
Manihot esculenta (cassava) contains two cyanogenic glucosides, linamarin and lotaustralin, biosynthesized from l-valine l-isoleucine, respectively. In this study, cDNAs encoding uridine diphosphate glycosyltransferase (UGT) paralogs, assigned the names UGT85K4 UGT85K5, have been isolated cassava. The paralogs display 96% amino acid identity, belong to a family containing glucoside-specific UGTs Sorghum bicolor Prunus dulcis. Recombinant UGT85K5 produced in Escherichia coli were able...
Lupins (Lupinus spp.) are nitrogen-fixing legumes that accumulate toxic alkaloids in their protein-rich beans. These anti-nutritional compounds belong to the family of quinolizidine (QAs), which interest pharmaceutical and chemical industries. To unleash potential lupins as protein crops sources QAs, a thorough understanding QA pathway is needed. However, only first enzyme pathway, lysine decarboxylase (LDC), known. Here, we report transcriptome high-QA variety narrow-leafed lupin (L....