A5035239204- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Biochemical and biochemical processes
- Microbial Metabolic Engineering and Bioproduction
- Natural product bioactivities and synthesis
- Plant Gene Expression Analysis
- Fungal and yeast genetics research
- Essential Oils and Antimicrobial Activity
- Photosynthetic Processes and Mechanisms
- Phytochemicals and Medicinal Plants
- Phytochemicals and Antioxidant Activities
- Transgenic Plants and Applications
- Bee Products Chemical Analysis
- Genomics, phytochemicals, and oxidative stress
- Polyomavirus and related diseases
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- CRISPR and Genetic Engineering
- Cervical Cancer and HPV Research
- Antioxidant Activity and Oxidative Stress
- Plant and animal studies
- Natural Compounds in Disease Treatment
- Traditional Chinese Medicine Analysis
- Neonatal Health and Biochemistry
- Nanoparticles: synthesis and applications
McGill University
2023-2024
University of Copenhagen
2017-2023
University of Crete
2011-2016
Mediterranean Agronomic Institute of Chania
2011-2014
Monoterpenes have an established use in the food and cosmetic industries recently also found application as advanced biofuels. Although metabolic engineering efforts so far achieved significant yields of larger terpenes, monoterpene productivity is lagging behind. Here, we set out to establish a monoterpene-specific production platform Saccharomyces cerevisiae identified sequential reaction mechanism yeast farnesyl diphosphate synthase Erg20p be important factor limiting yield. To overcome...
Significance Monoterpenoids, monoterpene indole alkaloids, and cannabinoids are highly valued for their fragrant therapeutic properties, but sourcing them from nature or deriving petrochemicals is no longer sustainable. However, sustainable production of these compounds in engineered microorganisms mostly hampered by the limited availability main building block biosynthesis, geranyl diphosphate. Here, we overcome this challenge engineering yeast peroxisomes as diphosphate-synthesizing...
Abstract Background Terpenoids constitute a large family of natural products, attracting commercial interest for variety uses as flavours, fragrances, drugs and alternative fuels. Saccharomyces cerevisiae offers versatile cell factory, the precursors terpenoid biosynthesis are naturally synthesized by sterol biosynthetic pathway. Results S. wild type yeast cells, selected their capacity to produce high levels were targeted improvement aiming increase production. Recyclable integration...
Synthetic biology approaches achieving the reconstruction of specific plant natural product biosynthetic pathways in dedicated microbial "chassis" have provided access to important industrial compounds (e.g., artemisinin, resveratrol, vanillin). However, potential such production systems facilitate elucidation has been underexplored. Here we report on application a modular terpene platform characterization pathway leading potent antioxidant carnosic acid and related diterpenes Salvia...
Abstract Synthetic biology efforts for the production of valuable chemicals are frequently hindered by structure and regulation native metabolic pathways chassis. This is particularly evident in case monoterpenoid Saccharomyces cerevisiae , where canonical terpene precursor geranyl diphosphate tightly coupled to biosynthesis isoprenoid compounds essential yeast viability. Here, we establish a synthetic orthogonal pathway based on an alternative precursor, neryl diphosphate. We identify...
Abstract Humankind relies on specialized metabolites for medicines, flavors, fragrances, and numerous other valuable biomaterials. However, the chemical space occupied by metabolites, and, thus, their application potential, is limited because biosynthesis based only a handful of building blocks. Engineering organisms to synthesize alternative blocks will bypass this limitation enable sustainable production molecules with non-canonical structures, expanding possible applications. Herein, we...
Terpenes are a large class of natural products, many which used in cosmetics, pharmaceuticals, or biofuels. However, terpene's industrial application is frequently hindered by limited availability sources low yields chemical synthesis. In this report, we developed modular platform based on standardized and exchangeable parts to reproduce potentially expand the diversity terpene structures Saccharomyces cerevisiae. By combining different module-specific parts, exploited substrate promiscuity...
Terpenoids (isoprenoids) have numerous applications in flavors, fragrances, drugs and biofuels. The number of microbially produced terpenoids is increasing as new biosynthetic pathways are being elucidated. However, efforts to improve terpenoid production yeast mostly taken advantage existing knowledge the sterol pathway, while many additional factors may affect output engineered system.Aiming develop a strain that can support high titers sclareol, diterpene great importance for perfume...
Synthetic biology is a rapidly emerging field with broad underlying applications in health, industry, agriculture, or environment, enabling sustainable solutions for unmet needs of modern society. With the very recent addition artificial intelligence (AI) approaches, this now growing at rate that can help reach envisioned goals bio-based society within next few decades. Integrating AI plant-based technologies, such as protein engineering, phytochemicals production, plant system microbiome...
Salvia diterpenes have been found to health promoting properties. Among them, carnosic acid and carnosol, tanshinones sclareol are well known for their cardiovascular, antitumor, antiinflammatory antioxidant activities. However, many of these compounds not available at a constant supply developing biotechnological methods production could provide sustainable alternative. The transcriptome S.pomifera glandular trichomes was analysed aiming identify genes that be used in the engineering...
Abstract Background Terpenoids and isoprenoids are an important class of natural products, which includes currently used drugs, high value bioactive industrial compounds, fuel candidates. Due to their application, there is increasing interest in the development S. cerevisiae strains capable producing levels terpenoids. Results Aiming identify new gene targets can be manipulated increase sesquiterpene production, a set HMG2 positive genetic interactors were assessed as single digenic...
Abstract Background Celastrol is a promising anti-obesity agent that acts as sensitizer of the protein hormone leptin. Despite its potent activity, sustainable source celastrol and derivatives for further pharmacological studies lacking. Results To elucidate biosynthetic pathway reconstruct it in Saccharomyces cerevisiae , we mined root-transcriptome Tripterygium wilfordii identified four oxidosqualene cyclases 49 cytochrome P450s candidates to be involved early steps biosynthesis. Using...
Solanum elaeagnifolium, an invasive weed of the Solanaceae family, is poorly studied although it poses a significant threat to crops. Here analysis transcriptome S. elaeagnifolium presented, as means explore biology this species and identify genes related its adaptation environmental stress. One basic mechanisms by which plants respond stress through synthesis specific secondary metabolites that protect plant from herbivores microorganisms, or serve signaling molecules. important such group...
Abstract Plants synthesize numerous specialized metabolites (also termed natural products) to mediate dynamic interactions with their surroundings. The complexity of plant metabolism is the result an inherent biosynthetic plasticity rooted in substrate and product promiscuity enzymes involved. pathway carnosic acid-related diterpenes rosemary sage involves promiscuous cytochrome P450s whose combined activity results a multitude structurally related compounds. Some these minor products, such...
Summary Diatoms are eukaryotic, unicellular algae that responsible for c . 20% of the Earth's primary production. Their dominance and success in contemporary oceans have prompted investigations on their distinctive metabolism physiology. One metabolic pathway remains largely unexplored diatoms is isoprenoid biosynthesis, which production numerous molecules with unique features. We selected diatom species Haslea ostrearia because its characteristic content carried out a comprehensive...
Earlier studies had suggested that epigenetic mechanisms play an important role in the control of human cytomegalovirus (HCMV) infection. Here we show productive HCMV infection is indeed under histone H3K27 trimethylation. The methyltransferase EZH2, and its regulators JARID2 NDY1/KDM2B repress GFI1, a transcriptional repressor major immediate-early promoter (MIEP) HCMV. Knocking down or relieves repression results upregulation GFI1. During infection, incoming rapidly downregulates GFI1 mRNA...
Several plant diterpenes have important biological properties. Among them, forskolin is a complex labdane-type diterpene whose activity stems from its ability to activate adenylyl cyclase and elevate intracellular cAMP levels. As such, it used in the control of blood pressure, protection congestive heart failure, weight-loss supplements. Chemical synthesis challenging, production engineered microbes could provide sustainable source. To this end, we set out establish platform for related...