A5011214266- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Renal and related cancers
- Angiogenesis and VEGF in Cancer
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Prostate Cancer Treatment and Research
- Nuclear Structure and Function
- Renal cell carcinoma treatment
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Genetic and Kidney Cyst Diseases
- Plant Stress Responses and Tolerance
- Hippo pathway signaling and YAP/TAZ
- MicroRNA in disease regulation
- Sulfur Compounds in Biology
- Phytochemicals and Antioxidant Activities
- RNA Interference and Gene Delivery
- Tea Polyphenols and Effects
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Tannin, Tannase and Anticancer Activities
- Melanoma and MAPK Pathways
University of the West of England
2014-2024
Frenchay Hospital
2019
University of St Andrews
1994-2015
Genomics (United Kingdom)
2006-2008
Centre for Life
2006
Dalhousie University
2006
Western General Hospital
1997-2003
Vascular endothelial growth factor A (VEGFA; hereafter referred to as VEGF) is a key regulator of physiological and pathological angiogenesis. Two families VEGF isoforms are generated by alternate splice-site selection in the terminal exon. Proximal (PSS) exon 8 results pro-angiogenic VEGFxxx (xxx number amino acids), whereas distal (DSS) anti-angiogenic VEGFxxxb isoforms. To investigate control PSS DSS, we investigated regulation isoform expression extracellular administration intracellular...
Vascular endothelial growth factor (VEGF) is produced either as a pro-angiogenic or anti-angiogenic protein depending upon splice site choice in the terminal, eighth exon. Proximal selection (PSS) exon 8 generates isoforms such VEGF(165), and distal (DSS) results VEGF(165)b. Cellular decisions on depend activity of RNA-binding factors, ASF/SF2, which have previously been shown to regulate VEGF choice. To determine mechanism by mediated, we investigated effect inhibition ASF/SF2...
Wt1 is a tumour suppressor gene, mutation of which cause Wilms' tumour, childhood renal nephroblastoma. expressed in rich pattern during development suggesting that it acts at three stages: determination the kidney area, differentiation nephrons and maturation glomeruli. Wt1−/− mice confirm essential for inception development; cells ought to form kidneys die by apoptosis instead. Specific human WT1 mutations defects glomerular (Denys–Drash Frasier syndromes), providing circumstantial...
In investigating the composition of stored (maternal) mRNP particles in Xenopus oocytes, attention has focussed primarily on phosphoproteins pp60/56, which are Y-box proteins involved a general packaging mRNA. We now identify third, abundant, integral component particles, Xp54, belongs to family DEAD-box RNA helicases. Xp54 was first detected by its ability photocrosslink ATP. Subsequent sequence analysis identifies as member helicase subfamily includes: human p54, encoded at chromosomal...
Mounting evidence suggests that one of the ways cells adapt to hypoxia is through alternative splicing. The aim this study was firstly examine effect on splicing cancer associated genes using prostate cell line PC3 as a model. Secondly, expression several regulators examined. were grown in 1% oxygen hypoxic chamber for 48 h, RNA extracted and sent high throughput PCR analysis at RNomics platform University Sherbrooke, Canada. Genes whose exon inclusion rate PSI (ψ) changed significantly...
The angiogenic capability of colorectal carcinomas (CRC), and their susceptibility to anti‐angiogenic therapy, is determined by expression vascular endothelial growth factor (VEGF) isoforms. intracellular protein T‐cell Intracellular Antigen (TIA‐1) alters post‐transcriptional RNA processing binds VEGF‐A mRNA. We therefore tested the hypothesis that TIA‐1 could regulate isoform in cancers. was measured cancers cell lines. discovered an endogenous splice variant encoding a truncated protein,...
The Wilms’ tumor 1 (WT1) gene plays an important role in mammalian urogenital development, and dysregulation of this is observed many human cancers. Alternative splicing WT1 RNA leads to the expression two major protein isoforms, WT1(+KTS) WT1(−KTS). Whereas WT1(−KTS) acts as a transcriptional regulator, no clear function has been ascribed WT1(+KTS), despite fact that crucial for normal development. Here we show functions enhance from possessing retained intron containing either cellular or...
Growing evidence suggests that the flavonoid epigallocatechin-3-gallate (EGCG), notably abundant in green tea, has health-promoting properties. We examined effect of EGCG on cell survival and apoptosis prostate cancer line PC3. Cell was reduced increased significantly with a low dose 1 µM EGCG. The ability anticancer drug cisplatin to promote enhanced by Furthermore, EGCG, both alone combination cisplatin, promoted expression pro-apoptotic splice isoform caspase 9.