A5031303879- Biofuel production and bioconversion
- Plant Gene Expression Analysis
- Microbial Metabolic Engineering and Bioproduction
- Lignin and Wood Chemistry
- Biochemical and biochemical processes
- Catalysis for Biomass Conversion
- Bioenergy crop production and management
- Polysaccharides and Plant Cell Walls
- Plant biochemistry and biosynthesis
- Horticultural and Viticultural Research
- Folate and B Vitamins Research
- Plant-Microbe Interactions and Immunity
- Metabolism and Genetic Disorders
- Photosynthetic Processes and Mechanisms
- Plant and fungal interactions
- Hymenoptera taxonomy and phylogeny
- Amino Acid Enzymes and Metabolism
- Plant Parasitism and Resistance
- Mycorrhizal Fungi and Plant Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Plant nutrient uptake and metabolism
- Fungal Biology and Applications
- Enzyme-mediated dye degradation
- Enzyme Structure and Function
- Biochemical and Molecular Research
Joint BioEnergy Institute
2015-2024
Lawrence Berkeley National Laboratory
2015-2024
Emery Oleochemicals (Malaysia)
2020
University of Florida
2008-2009
Institut National de la Recherche Agronomique
2008
Laboratoire de Génétique Cellulaire
2003-2006
Natural Resources Canada
2003-2005
Institut Jean-Pierre Bourgin
2005
Canadian Forest Service
2003
Abstract During lignin biosynthesis in angiosperms, coniferyl and sinapyl aldehydes are believed to be converted into their corresponding alcohols by cinnamyl alcohol dehydrogenase (CAD) (SAD), respectively. This work clearly shows that CAD-C CAD-D act as the primary genes involved floral stem of Arabidopsis thaliana supplying both alcohols. An CAD double mutant (cad-c cad-d) resulted a phenotype with limp at maturity well modifications pattern staining. Lignin content was reduced 40%, 94%...
Phenolic cross-links and inter-unit linkages result from the oxidative coupling of hydroxycinnamates leading to lignin assembly cross-linking with cell wall polysaccharides extensin proteins.
The specific and tightly controlled transport of numerous nutrients metabolites across cellular membranes is crucial to all forms life. However, many the transporter proteins involved have yet be identified, including vitamin transporters in various human pathogens, whose growth depends strictly on uptake. Comparative analysis ever-growing collection microbial genomes coupled with experimental validation enables discovery such transporters. Here, we used this approach discover an abundant...
Summary Lignocellulosic biomass is utilized as a renewable feedstock in various agro‐industrial activities. Lignin an aromatic, hydrophobic and mildly branched polymer integrally associated with polysaccharides within the biomass, which negatively affects their extraction hydrolysis during industrial processing. Engineering monomer composition of lignins offers attractive option towards new reduced recalcitrance. The presented work describes strategy developed Arabidopsis for overproduction...
Naturally, many aerobic organisms degrade lignin-derived aromatics through conserved intermediates including protocatechuate and catechol. Employing this microbial approach offers a potential solution for valorizing lignin into valuable chemicals lignocellulosic biorefinery enabling bioeconomy. In study, two hybrid biochemical routes combining chemical depolymerization, plant metabolic engineering, synthetic pathway reconstruction were demonstrated value-added products. the route 1, alkali...
The future bioeconomy promises drop-in or performance-advantaged biofuels and bioproducts derived from lignocellulosic biomass, substantial greenhouse gas emissions reductions in sectors with few no alternatives, increased domestic energy production countries sufficient biomass resources. Despite the slower than anticipated pace of commercializing next-generation biofuels, research community continues to make dramatic improvements at every stage production, feedstock cultivation through...
Summary Lignin confers recalcitrance to plant biomass used as feedstocks in agro‐processing industries or source of renewable sugars for the production bioproducts. The metabolic steps synthesis lignin building blocks belong shikimate and phenylpropanoid pathways. Genetic engineering efforts reduce content typically employ gene knockout silencing techniques constitutively repress one these Recently, new strategies have emerged offering better spatiotemporal control deposition, including...
Lignin poses a major challenge in the processing of plant biomass for agro-industrial applications. For bioengineering purposes, there is pressing interest identifying and characterizing enzymes responsible biosynthesis lignin. Hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl transferase (HCT; EC 2.3.1.133) key metabolic entry point synthesis most important lignin monomers: coniferyl sinapyl alcohols. In this study, we investigated substrate promiscuity HCT from bryophyte ( Physcomitrella )...
Significance Deep eutectic solvents (DESs) have gained increasing attention due to their application-friendly properties, including universal solvating capabilities and wide tunability. Additionally, ease of synthesis broad availability from inexpensive chemical components could render DESs more versatile for biomass pretreatment, as compared with traditional ionic liquids. Because the long-term success biorefinery depends on development sustainable processes convert lignocellulosics into...
Abstract Studying Arabidopsis mutants of the phenylpropanoid pathway has unraveled several biosynthetic steps monolignol synthesis. Most genes leading to synthesis have been characterized recently in this herbaceous plant, except those encoding cinnamyl alcohol dehydrogenase (CAD). We used complete sequencing genome highlight a new view CAD gene family. Among nine AtCAD genes, we identified two distinct paralogs AtCAD-C and AtCAD-D, which share 75% identity are likely be involved lignin...
Lignin in plant biomass represents a target for engineering strategies towards the development of sustainable bioeconomy. In addition to conventional lignin monomers, namely p-coumaryl, coniferyl and sinapyl alcohols, tricin has been shown be part native polymer certain monocot species. Because is considered initiate polymerization chains, elucidating its biosynthesis mechanism export cell wall constitute novel challenges bioenergy crops. Late steps require two methylation reactions...
Lignocellulosic biomass is a highly sustainable and largely carbon dioxide neutral feedstock for the production of biofuels advanced biomaterials. Although thermochemical pretreatment typically used to increase efficiency cell wall deconstruction, genetic engineering major plant polymers, especially lignin, has shown promise as an alternative approach reduce recalcitrance. Poplar trees with reduced lignin content altered composition were previously developed by overexpressing bacterial...
Abstract Background Oats contain hydroxycinnamoyl anthranilates, also named avenanthramides (Avn), which have beneficial health properties because of their antioxidant, anti-inflammatory, and antiproliferative effects. The microbial production anthranilates is an eco-friendly alternative to chemical synthesis or purification from plant sources. We recently demonstrated in yeast ( Saccharomyces cerevisiae ) that coexpression 4-coumarate: CoA ligase (4CL) Arabidopsis thaliana...
Single guide RNA (sgRNA) selection is important for the efficiency of CRISPR/Cas9-mediated genome editing. However, in plants, rules governing are not well established. We developed a facile transient assay to screen sgRNA efficiency. then used it test top-performing bioinformatically predicted sgRNAs two different Arabidopsis genes. In our assay, these had vastly editing efficiencies, and efficiencies were replicated stably transformed lines. One genes, hydroxycinnamoyl-CoA...
Renewed interests in the development of bioenergy, biochemicals, and biomaterials have elicited new strategies for engineering lignin biomass feedstock plants. This study shows, first time, that 3,4-dihydroxybenzoate (DHB) is compatible with radical coupling reactions assemble polymeric We introduced a bacterial 3-dehydroshikimate dehydratase into hybrid poplar (Populus alba × grandidentata) to divert carbon flux away from shikimate pathway, which lies upstream biosynthesis. Transgenic wood...
Abstract The industrial amino acid production workhorse, Corynebacterium glutamicum naturally produces low levels of 2,3,5,6-tetramethylpyrazine (TMP), a valuable flavor, fragrance, and commodity chemical. Here, we demonstrate TMP (∼0.8 g L−1) in C. type strain ATCC13032 via overexpression acetolactate synthase and/or α-acetolactate decarboxylase from Lactococcus lactis CGXII minimal medium supplemented with 40 L−1 glucose. This engineered also demonstrated growth when the was up to 40% (v...
Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression OsAT10, BAHD acyltransferase gene, enhances saccharification efficiency in rice. Here we show rice OsAT10 gene decreased levels wall-bound ferulic acid (FA)...
We previously developed several strategies to engineer plants produce cost-efficient biofuels from plant biomass. Engineered Arabidopsis with low xylan and lignin content showed normal growth improved saccharification efficiency under standard conditions. However, it remains be determined whether these engineered perform well drought stress, which is the primary source of abiotic stress in field.Upon exposing severe drought, we observed better survival rates those a degree acetylation,...
Abstract Background Plant cell walls are interwoven structures recalcitrant to degradation. Native and adapted microbiomes can be particularly effective at plant wall deconstruction. Although most understanding of biological deconstruction has been obtained from isolates, cultivated that break down have emerged as new sources for biotechnologically relevant microbes enzymes. These provide a unique resource identify key interacting functional microbial groups guide the design specialized...