A5077618789- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Lignin and Wood Chemistry
- Catalysis for Biomass Conversion
- Bioenergy crop production and management
- Odor and Emission Control Technologies
- Enzyme Production and Characterization
- Advanced Cellulose Research Studies
- Bioeconomy and Sustainability Development
- Polysaccharides and Plant Cell Walls
- Catalysis and Hydrodesulfurization Studies
- Wastewater Treatment and Nitrogen Removal
- Microbial Metabolites in Food Biotechnology
- Microbial bioremediation and biosurfactants
- Zeolite Catalysis and Synthesis
- Plant Gene Expression Analysis
- Force Microscopy Techniques and Applications
- Enzyme-mediated dye degradation
- Forest Biomass Utilization and Management
- Viral Infectious Diseases and Gene Expression in Insects
- Biotechnology and Related Fields
- Anaerobic Digestion and Biogas Production
- Nuclear Physics and Applications
- Nuclear reactor physics and engineering
Oak Ridge National Laboratory
2016-2025
Office of Scientific and Technical Information
2019-2024
Government of the United States of America
2022-2023
National Technical Information Service
2019-2021
University of Tennessee at Knoxville
1997-2020
Oak Ridge Associated Universities
2017-2020
Bioenergy Life Science (United States)
2009-2016
Imperial College London
2006
Georgia Institute of Technology
2006
University of British Columbia
2006
To the Editor: Over past two decades, scale and complexity of genomics technologies data have advanced from sequencing genomes a few organisms to generating metagenomes, genome variation, gene expression, metabolites, phenotype for thousands their communities.A major challenge in this data-rich age biology is integrating heterogeneous distributed into predictive models biological function, ranging single entire ecologies.The US Department Energy (DOE) has invested substantially efforts...
Abstract The production of cellulosic ethanol from biomass is considered a promising alternative to reliance on diminishing supplies fossil fuels, providing sustainable option for fuels in an environmentally compatible manner. conversion lignocellulosic biofuels through biological route usually suffers the intrinsic recalcitrance owing complicated structure plant cell walls. Currently, pretreatment step that can effectively reduce generally required make polysaccharide fractions locked...
The primary obstacle to producing renewable fuels from lignocellulosic biomass is a plant's recalcitrance releasing sugars bound in the cell wall. From sample set of wood cores representing 1,100 individual undomesticated Populus trichocarpa trees, 47 extreme phenotypes were selected across measured lignin content and ratio syringyl guaiacyl units (S/G ratio). This subset was tested for total sugar release through enzymatic hydrolysis alone as well combined hot-water pretreatment using...
Lignocellulosic biomass, a potentially important renewable organic source of energy and chemical feedstock, resists degradation to glucose in industrial hydrolysis processes thus requires expensive thermochemical pretreatments. Understanding the mechanism biomass breakdown during these pretreatments will lead more efficient use biomass. By combining multiple probes structure, sensitive different length scales, with molecular dynamics simulations, we reveal two fundamental responsible for...
Hybrid processes, featuring biological conversion of lignocellulose to small molecules followed by chemo-catalytic larger suitable for difficult-to-electrify transport modes, are a promising route biomass-derived fuels in demand climate stabilization.
Abstract A unique nanoporous sol‐gel glass possessing a highly ordered porous structure (with pore size of 153 Å in diameter) was examined for use as support material enzyme immobilization. model enzyme, α‐chymotrypsin, efficiently bound onto the via bifunctional ligand, trimethoxysilylpropanal, with an active loading 0.54 wt%. The glass‐bound chymotrypsin exhibited greatly enhanced stability both aqueous solution and organic solvents. half‐life α‐chymotrypsin >1000‐fold higher than that...
Abstract Background Zymomonas mobilis ZM4 (ZM4) produces near theoretical yields of ethanol with high specific productivity and recombinant strains are able to ferment both C-5 C-6 sugars. Z. performs best under anaerobic conditions, but is an aerotolerant organism. However, the genetic physiological basis ZM4's response various stresses understood poorly. Results In this study, transcriptomic metabolomic profiles for aerobic fermentations were elucidated by microarray analysis...
Lignin S/G ratio has been investigated as an important factor in biomass recalcitrance to bioethanol production. Because of the complexity and variety biomass, was also reportedly influenced by several other factors, such total lignin content, degree cellulose polymerization, etc. In addition, effect on conversion is not uniform across plant species. Herein, 11 Populus trichocarpa natural variants grown under same conditions with similar content were selected minimize effects factors. The P....
Interactions of water with cellulose are both fundamental and technological importance. Here, we characterize the properties associated using deuterium labeling, neutron scattering molecular dynamics simulation. Quasi-elastic provided quantitative details about dynamical relaxation processes that occur was supported by structural characterization small-angle X-ray diffraction. We can unambiguously detect two populations cellulose. The first is "non-freezing bound" gradually becomes mobile...
Feedstock recalcitrance is the most important barrier impeding cost-effective production of cellulosic biofuels. Pioneer commercial ethanol facilities employ thermochemical pretreatment and addition fungal cellulase, reflecting main research emphasis in field. However, it has been suggested that may be possible to process biomass without using thermophilic, cellulolytic bacteria. To further explore this idea, we examine ability various biocatalysts solubilize autoclaved but otherwise...
The recalcitrance of natural<italic>Populus</italic>variants was elucidated.
The recalcitrance of cellulosic biomass is widely recognized as a key barrier to cost-effective biological processing fuels and chemicals, but the relative impacts physical, chemical genetic interventions improve singly in combination have yet be evaluated systematically. Solubilization plant cell walls can enhanced by non-biological augmentation including physical cotreatment thermochemical pretreatment, choice biocatalyst, feedstock, engineering plants, choosing feedstocks that are less...
Abstract 21st‐century modeling of greenhouse gas (GHG) emissions from bioenergy crops is necessary to quantify the extent which production can mitigate climate change. For over 30 years, Century‐based biogeochemical models have provided preeminent framework for belowground carbon and nitrogen cycling in ecosystem earth system models. While monthly Century daily time‐step version (DayCent) advanced our ability predict sustainability crop production, new advances feedstock generation,...
First-of-its-kind effort to understand full-scale multi-product biorefineries established upon co-solvent enhanced lignocellulosic fractionation (CELF) from an economic and environmental standpoint.
Down-regulation of the caffeic acid 3-O-methyltransferase EC 2.1.1.68 (COMT) gene in lignin biosynthetic pathway switchgrass (Panicum virgatum) resulted cell walls transgenic plants releasing more constituent sugars after pretreatment by dilute and treatment with glycosyl hydrolases from an added enzyme preparation Clostridium thermocellum. Fermentation both wild-type milder hot water no washing showed that only inhibited C. Gas chromatography–mass spectrometry (GCMS)-based metabolomics were...
The exchanged Ga(<sc>iii</sc>) sites are the active for promoting BTX formation during ethanol conversion.