A5054531448- Bioenergy crop production and management
- Plant Molecular Biology Research
- Biofuel production and bioconversion
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant and fungal interactions
- Plant Taxonomy and Phylogenetics
- Turfgrass Adaptation and Management
- Legume Nitrogen Fixing Symbiosis
- Myasthenia Gravis and Thymoma
- Genetic Mapping and Diversity in Plants and Animals
- Peripheral Neuropathies and Disorders
- Ruminant Nutrition and Digestive Physiology
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Seed Germination and Physiology
- CRISPR and Genetic Engineering
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Chromosomal and Genetic Variations
- Soybean genetics and cultivation
- Aluminum Alloy Microstructure Properties
Qingdao Agricultural University
2019-2025
Shanghai Jiao Tong University
2023-2025
Noble Research Institute
2012-2024
Fudan University
2023-2024
Zhongshan Hospital
2023-2024
State Forestry and Grassland Administration
2022-2024
China Tobacco
2024
Berry Oncology (China)
2023
Genomics (United Kingdom)
2023
Jiangnan University
2022
Abstract The evolution of lignin biosynthesis was critical in the transition plants from an aquatic to upright terrestrial lifestyle. Lignin is assembled by oxidative polymerization two major monomers, coniferyl alcohol and sinapyl alcohol. Although recently discovered laccases, LAC4 LAC17, have been shown play a role Arabidopsis thaliana, disruption both genes only leads relatively small change content under continuous illumination. Simultaneous LAC11 along with LAC17 causes severe plant...
Summary The identification of leaf wax genes involved in stress tolerance is expected to have great potential for crop improvement. Here we report the characterization a novel AP2 domain‐containing putative transcription factor gene from model legume Medicago truncatula. gene, designated WXP1 , able activate w x p roduction and confer drought alfalfa ( sativa ), most important forage species world close relative M. truncatula . predicted protein has 371 aa; it one longest peptides all single...
Summary Switchgrass ( Panicum virgatum L.) has been developed into a dedicated herbaceous bioenergy crop. Biomass yield is major target trait for genetic improvement of switchgrass. microRNAs have emerged as prominent class gene regulatory factors that the potential to improve complex traits such biomass yield. A miR156b precursor was overexpressed in The effects miR156 overexpression on SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) genes were revealed by microarray and quantitative RT‐PCR...
Summary Proanthocyanidins (PAs) and their monomeric building blocks, the (epi)‐flavan‐3‐ols, are plant antioxidants that confer multiple human health benefits. The presence of PAs in forage crops is an important agronomic trait, preventing pasture bloat ruminant animals. However, many consumed materials lack PAs, there has been little success to date introducing or polymeric flavan‐3‐ols de novo into tissues for disease prevention by dietary means development ‘bloat‐safe’ forages. We report...
Oligomeric proanthocyanidins (PAs) composed primarily of epicatechin units accumulate in the seed coats model legume Medicago truncatula, reaching maximal levels at around 20 d after pollination. Genes encoding single anthocyanidin synthase (ANS; EC 1.14.11.19) and leucoanthocyanidin reductase (LAR; 1.17.1.3) were cloned corresponding enzymes functionally identified. Recombinant MtANS converted leucocyanidin to cyanidin, and, more efficiently, dihydroquercetin flavonol quercetin. Levels...
Summary S ‐adenosylmethionine synthetase ( SAMS ) is the key enzyme catalysing formation of SAM ), a precursor polyamines and ethylene. To investigate potential role in cold tolerance, we isolated Mf 1 from cold‐tolerant germplasm Medicago sativa subsp. falcata analysed association ‐derived with tolerance. The expression leaves was greatly induced by cold, abscisic acid ABA H 2 O nitric oxide NO ). Our data revealed that , interactions mediated cold‐induced acclimation . putrescine,...
Abstract Medicago truncatula has been developed into a model legume. Its close relative alfalfa (Medicago sativa) is the most widely grown forage legume crop in United States. By screening large population of M. mutants tagged with transposable element tobacco (Nicotiana tabacum) cell type1 (Tnt1), we identified mutant line (NF2089) that maintained green leaves and showed anthers, central carpels, mature pods, seeds during senescence. Genetic molecular analyses revealed mutation was caused...
Alfalfa is the most extensively cultivated forage legume. Salinity a major environmental factor that impacts on alfalfa's productivity. However, little known about molecular mechanisms underlying alfalfa responses to salinity, especially relative contribution of two important components osmotic and ionic stress. In this study, we constructed first full-length transcriptome database for root tips under continuous NaCl mannitol treatments 1, 3, 6, 12, 24 h (three biological replicates each...
Summary Switchgrass ( Panicum virgatum L.) is a leading candidate for dedicated lignocellulosic biofuel feedstock owing to its high biomass production, wide adaptation and low agronomic input requirements. Lignin in cell walls of switchgrass, other feedstocks, severely limits the accessibility wall carbohydrates enzymatic breakdown into fermentable sugars subsequently biofuels. Low‐lignin transgenic switchgrass plants produced by down‐regulation caffeic acid O ‐methyltransferase COMT ),...
Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR associated protein 9 (Cas9) system allows effective gene modification through RNA-guided DNA targeting. The Cas9 has undergone a series of functional alterations from original active endonuclease to partially or completely deactivated Cas9. catalytically (dCas9) offers platform regulate transcriptional expression with addition activator repressor domains. We redesigned CRISPR/Cas9 activation by adding p65...
Summary Biomass yield, salt tolerance and drought are important targets for alfalfa ( Medicago sativa L.) improvement. truncatula has been developed into a model plant other legumes. By screening Tnt1 retrotransposon‐tagged M. mutant population, we identified three mutants with enhanced branching. Branch development determines shoot architecture which affects functions such as light acquisition, resource use ultimately impacts biomass production. Molecular analyses revealed that the...
The development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (Panicum virgatum). Switchgrass is an outcrossing species with allotetraploid (2n = 4x 36), a complexity which forms impediment to generating homozygous knock-out plants. Lignin, major component the plant cell wall and contributor cellulosic feedstock's recalcitrance decomposition, stands as barrier efficient biofuel production by limiting enzyme access polymers during...
The recalcitrance of cellulosic biomass is widely recognized as a key barrier to cost-effective biological processing fuels and chemicals, but the relative impacts physical, chemical genetic interventions improve singly in combination have yet be evaluated systematically. Solubilization plant cell walls can enhanced by non-biological augmentation including physical cotreatment thermochemical pretreatment, choice biocatalyst, feedstock, engineering plants, choosing feedstocks that are less...
Alfalfa (Medicago sativa L.) is a perennial flowering plant in the legume family that widely cultivated as forage crop for its high yield, quality and related agricultural economic benefits. photoperiod sensitive long-day (LD) can accomplish vegetative reproductive phases short period of time. However, rapid compromise biomass yield quality. Here, we attempted to delay alfalfa using multiplex CRISPR/Cas9-mediated mutagenesis FLOWERING LOCUS Ta1 (MsFTa1), key floral integrator activator gene....
Lignification of cell walls during plant development has been identified as the major factor limiting forage digestibility and concomitantly animal productivity. cDNA sequences encoding a key lignin biosynthetic enzyme, cinnamyl alcohol dehydrogenase (CAD), were cloned from widely grown monocotyledonous species tall fescue (Festuca arundinacea Schreb.). Recombinant CAD expressed in E. coli exhibited highest V(max)/K(m) values when coniferaldehyde sinapaldehyde used substrates. Transgenic...
Stem tissues of tall fescue (Festuca arundinacea Schreb.) were sampled at three elongation stages and reproductive stages. Anatomical analysis showed the deposition guaiacyl (G) syringyl (S) lignin during plant development formation a lignified sclerenchyma ring. A dramatic increase in Klason content was found from stage to stage. Lignin composition analyzed by gas chromatography−mass spectrometry revealed that S S/G ratio increased with stem development, but contents p-hydroxyphenyl (H) G...
Dry matter digestibility is one of the most important characteristics forage. The major constraint on ruminant digestion forage cell walls lignin. Sequences cDNA encoding a key lignin biosynthetic enzyme, caffeic acid O-methyltransferase (COMT), was cloned from widely grown monocot species tall fescue (Festuca arundinacea Schreb.). Enzymatic properties recombinant COMT protein expressed in E. coli were determined using six substrates. preferred substrates for 5-hydroxyferulic and caffeoyl...
Down-regulation of the caffeic acid 3-O-methyltransferase EC 2.1.1.68 (COMT) gene in lignin biosynthetic pathway switchgrass (Panicum virgatum) resulted cell walls transgenic plants releasing more constituent sugars after pretreatment by dilute and treatment with glycosyl hydrolases from an added enzyme preparation Clostridium thermocellum. Fermentation both wild-type milder hot water no washing showed that only inhibited C. Gas chromatography–mass spectrometry (GCMS)-based metabolomics were...
Abstract It is necessary to overcome recalcitrance of the biomass saccharification (sugar release) make switchgrass (Panicum virgatum) economically viable as a feedstock for liquid biofuels. Lignin content correlates negatively with sugar release efficiency in switchgrass, but selecting right gene candidates engineering lignin biosynthesis this tetraploid outcrossing species not straightforward. To assist endeavor, we have used an inducible cell suspension system studying response exogenous...
Switchgrass (Panicum virgatum L.) is a perennial grass species receiving significant focus as potential bioenergy crop. In the last 5 yr switchgrass research community has produced genetic linkage map, an expressed sequence tag (EST) database, set of single nucleotide polymorphism (SNP) markers that are distributed across 18 groups, 4x sampling P. AP13 genome in 400-bp reads, and bacterial artificial chromosome (BAC) libraries containing over 200,000 clones. These studies have revealed close...