A5081790753- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Phytochemicals and Antioxidant Activities
- Plant tissue culture and regeneration
- Genetic diversity and population structure
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Insect Resistance and Genetics
- Polysaccharides and Plant Cell Walls
- Ginkgo biloba and Cashew Applications
- Bioactive natural compounds
- Botanical Research and Chemistry
- Plant Reproductive Biology
- Soybean genetics and cultivation
- Phytochemical compounds biological activities
- Transgenic Plants and Applications
- Biofuel production and bioconversion
- Chromosomal and Genetic Variations
- Phytoestrogen effects and research
- Natural product bioactivities and synthesis
- CRISPR and Genetic Engineering
Sanya University
2025
Chinese Academy of Agricultural Sciences
2017-2024
Institute of Animal Sciences
2018-2022
University of Chinese Academy of Sciences
2021
Institute of Botany
2015-2017
Chinese Academy of Sciences
2015-2017
Beijing Botanical Garden
2017
Noble Research Institute
2007-2013
Guangxi Veterinary Research Institute
2012
Institut des Sciences du Végétal
2009
Oligomeric proanthocyanidins (PAs) composed primarily of epicatechin units accumulate in the seed coats model legume Medicago truncatula, reaching maximal levels at around 20 d after pollination. Genes encoding single anthocyanidin synthase (ANS; EC 1.14.11.19) and leucoanthocyanidin reductase (LAR; 1.17.1.3) were cloned corresponding enzymes functionally identified. Recombinant MtANS converted leucocyanidin to cyanidin, and, more efficiently, dihydroquercetin flavonol quercetin. Levels...
Expression of the Arabidopsis TRANSPARENT TESTA 2 (TT2) MYB family transcription factor leads to massive accumulation proanthocyanidins (PAs) in hairy roots Medicago truncatula. Microarray analysis showed that TT2 induces genes for flavonoid/PA biosynthesis, factors, and a large number unknown function. A second microarray dataset identified were preferentially expressed M. truncatula seed coat. Comparison two datasets defines target steps are yet unidentified PA biosynthesis accumulation....
WD40 repeat proteins regulate biosynthesis of anthocyanins, proanthocyanidins (PAs), and mucilage in the seed development trichomes root hairs. We have cloned characterized a protein gene from Medicago truncatula (MtWD40-1) via retrotransposon-tagging approach. Deficiency MtWD40-1 expression blocks accumulation range phenolic compounds, including PAs, epicatechin, other flavonoids, benzoic acids, seed, reduces epicatechin levels without corresponding effects on flavonoids flowers, isoflavone...
MYB transcription factors help to control anthocyanin biosynthesis in plants, and ectopic expression of the Arabidopsis Production Anthocyanin Pigment 1 (PAP1) factor activates pathway tobacco, suggesting general utility such for metabolic engineering anthocyanins anthocyanin-derived compounds as proanthocyanidins (condensed tannins). However, PAP1 does not activate model legume Medicago truncatula or alfalfa (Medicago sativa). A related Legume (LAP1) gene was identified from genome M....
Tea (Camellia sinensis) is rich in specialized metabolites, especially polyphenolic proanthocyanidins (PAs) and their precursors. To better understand the PA pathway tea, we generated a complementary DNA library from leaf tissue of blister blight-resistant tea cultivar TRI2043 functionally characterized key enzymes responsible for biosynthesis Structural genes encoding involved general phenylpropanoid/flavonoid PA-specific branch were well represented library. Recombinant leucoanthocyanidin...
The inducible CRISPR-activation (CRISPR-a) system offers unparalleled precision and versatility for regulating endogenous genes, making it highly sought after in plant research. In this study, we developed a chemically CRISPR-a tool called ER-Tag by combining the XVE with SunTag system. We systematically compared different induction strategies achieved high efficiency target gene activation. demonstrated that guide RNAs could be multiplexed pooled large-scale screening of effective...
Journal Article Antimicrobial susceptibilities and resistance genes in Campylobacter strains isolated from poultry pigs Australia Get access A.S. Obeng, Obeng School of Pharmacy Medical Sciences University South Adelaide SA AustraliaSansom Institute Search for other works by this author on: Oxford Academic Google Scholar H. Rickard, Rickard M. Sexton, Sexton Biosecurity Primary Industries Resources Glenside Y. Pang, Pang Guangxi Veterinary Research Nanning 53001 China Peng, Peng Barton...
Abstract Flavonoids are usually present in forms of glucosides plants, which could be catabolized by β ‐glucosidase (BGLU) to form their corresponding flavonoid aglycones. In this study, we isolated three abiotic‐responsive BGLU genes ( MtBGLU17, MtBGLU21 and MtBGLU22 ) from Medicago truncatula , found only the recombinant MtBGLU17 protein catalyse hydrolysis glycosides. The is active towards a variety glucosides, including flavones (apigenin luteolin), flavonols (kaempferol quercetin),...
SUMMARY Proanthocyanidins (PAs), a group of flavonoids, are found in leaves, flowers, fruits, and seed coats many plant species. PAs primarily composed epicatechin units the model legume species, Medicago truncatula . It can be synthesized from two separate pathways, leucoanthocyanidin reductase (MtLAR) pathway anthocyanidin synthase (MtANS) pathway, which produce through (MtANR). These pathways mainly controlled by MYB–bHLH–WD40 (MBW) ternary complex. Here, we characterize class IV...
Epimedium pubescens Maxim. is a well-known traditional Chinese medicinal herb with flavonol glycosides as the major pharmaceutically active compounds. UDP-glycosyltransferases (UGTs) are group of enzymes responsible for glycosylation flavonoid glycosides. In this study, genome-wide analysis was performed to identify UGT family genes in E. pubescens. As result, total 339 putative were identified, which represents largest gene known thus far, implying significant expansion All EpUGTs unevenly...
Glycine max L. accumulates a large amount of isoflavonoid compounds, which is beneficial for plant defense, plant-microbe symbiotic interactions, and human health. Several CYP450 subfamily genes are involved in the flavonoid biosynthetic pathway plants. In present study, we found 24 CYP82 were differentially expressed various tissues soybean, Phytophthora sojae-infected soybean varieties hairy roots treated with cell wall glucan elicitor. Six them (GmCYP82A2, GmCYP82A3, GmCYP82A4,...
Base editors offer potential for site saturation mutagenesis, yet deaminase-based are constrained to adenine and cytosine targets (Li et al., 2023a). Recently, glycosylase-based base (gBEs), which fuse engineered glycosylases with SpCas9 nickase (SpCas9n, D10A) excise specific guanine or thymine bases, achieve conversions through DNA repair over abasic sites (He 2024; Tong 2023; Ye 2024). While (gGBEs) show efficient conversion in plants (Liu Tian 2024), (TBEs) remain unexplored (Figure S1)....
Alfalfa (Medicago sativa L.) is an important legume forage crop with great economic value. However, as the growth of alfalfa seriously affected by inadequate supply water, drought probably major abiotic environmental factor that most severely affects production worldwide. In effort to enhance tolerance, we transformed Arabidopsis Enhanced Drought Tolerance 1 (AtEDT1) gene into via Agrobacterium-mediated transformation. Compared wild type plants, stress treatment resulted in higher survival...
β-glucosidases (BGLUs) hydrolyze the β-D-glycosidic bond with retention of anomeric configuration. BGLUs were associated many aspects plant physiological processes, in particular biotic and abiotic stresses through activation phytohormones defense compounds. However, studies on systematic analysis stress- or hormone-responsive BGLU genes are still rare. In this study, total 51 glycoside hydrolase family 1 identified genome model legume Medicago truncatula, they classified into five distinct...
The calcineurin B-like protein (CBL) and CBL-interacting kinase (CIPK) play important roles in plant signal transduction response to abiotic stress. Plants of Medicago genus contain many forages, their growth is often affected by a variety stresses. However, studies on the CBL CIPK family member function are rare Medicago. In this study, total 23 58 genes were identified from genome sativa as an forage crop, Medicaog truncatula model plant. Phylogenetic analysis suggested that these could be...
Proanthocyanidins (PAs) are the second most abundant plant phenolic natural products. PA biosynthesis is regulated by well-documented MYB/bHLH/WD40 (MBW) complex, but how this complex itself remains ill defined. Here, in situ hybridization and β-glucuronidase staining show that APETALA2 (AP2), a well-defined regulator of flower seed development, strongly expressed coat endothelium, where PAs accumulate. AP2 negatively regulates content expression levels key pathway genes. activates MYBL2...
Tea plant (Camellia sinensis) accumulates abundant flavonoid glycosides that are the major bioactive ingredients in tea. Biosynthesis of catalyzed by UDP-glucosyltransferases (UGTs) widely present plants. Among one hundred and seventy-eight UGTs genes we have previously identified tea plant, few them been functionally characterized. In study, further UGT73A17 gene is responsible for biosynthesis a broad range glycosides. Sequence analysis revealed deduced protein showed high identity with...
Glycine max is one of the most important grain and oil crops, improvement seed yield major objectives in soybean breeding. The AP2/ERF superfamily members are involved regulating flower development many species, therefore play key roles yield. However, it still unknown that how were presented G. genome whether these family function max. Here, we identified 380 genes genome. Phylogenetic analysis showed 323 grouped into ERF family, 49 AP2 family. Among 14 euAP2 lineage high identity with...