A5101644649- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Endoplasmic Reticulum Stress and Disease
- Enzyme Production and Characterization
- Plant Molecular Biology Research
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Influenza Virus Research Studies
- Plant Gene Expression Analysis
- Glycosylation and Glycoproteins Research
- Fermentation and Sensory Analysis
- Natural product bioactivities and synthesis
- Blood Coagulation and Thrombosis Mechanisms
- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Medicinal Plant Pharmacodynamics Research
Fudan University
2015-2025
Soochow University
2025
First Affiliated Hospital of Soochow University
2025
Shanghai Micro Satellite Engineering Center
2014-2025
State Key Laboratory of Genetic Engineering
2005-2024
First Affiliated Hospital of Xi'an Jiaotong University
2024
Nanjing Agricultural University
2017-2023
Harbin Medical University
2019-2023
Ministry of Agriculture and Rural Affairs
2021-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2022
A rationally designed graphene-hollow polypyrrole (PPy) nanoarchitecture in which hollow PPy spheres were inserted between graphene layers was constructed by mixing oxide and polystyrene (PS)@PPy core-shell sphere, followed reduction of etching PS. The as-prepared explored as electrode material for supercapacitor applications. specific capacitance may gradually rise to high 500 F g(-1) with a charging/discharging current density 5 g(-1), remains stable even after 10,000 cycles. Analysis...
Lariat RNAs formed as by-products of splicing are quickly degraded by the RNA debranching enzyme 1 (DBR1), leading to their turnover. Null dbr1 mutants in both animals and plants embryo lethal, but mechanism underlying lethality remains unclear. Here we characterized a weak mutant allele DBR1 Arabidopsis, dbr1-2, showed that global increase lariat was unexpectedly accompanied genome-wide reduction miRNA accumulation. The dbr1-2 mutation had no effects on expression biogenesis genes or...
Kluyveromyces marxianus, the known fastest-growing eukaryote on earth, has remarkable thermotolerance and capacity to utilize various agricultural residues produce low-cost bioethanol, hence is industrially important resolve imminent energy shortage crisis. Currently, poor ethanol tolerance hinders its operable application in industry, it necessary improve K. marxianus' resistance unravel underlying systematical mechanisms. However, this been seldom reported date. We carried out a wild-type...
Abstract Kluyveromyces marxianus is a food‐safe yeast with great potential for producing heterologous proteins. Improving the yield in K. remains challenge and incorporating large‐scale functional modules poses technical obstacle engineering. To address these issues, linear circular artificial chromosomes of (KmYACs) were constructed loaded disulfide bond formation from Pichia pastoris or . These contained up to seven genes maximum size 15 kb. KmYACs carried telomeres either Tetrahymena...
Taking into account its thermotolerance, high growth rate, and broad substrate spectrum, Kluyveromyces marxianus can be considered an ideal consolidated bioprocessing (CBP). A major obstacle to ethanol production using K. is the low of lignocellulolytic enzymes, which reduces cellulose hydrolysis production. Thus, further improvement enzyme expression secretion essential. To improve inulinase promoter signal sequence from was optimized through mutagenesis. T(-361)A mutation inside promoter,...
Posttranslational modification of p53 is a critical event in regulating the expression its target genes. acetylated at lysine 120 (K120) by acetyltranferases Tip60 (KAT5) and hMOF (KAT8) response to DNA damage. Identification cofactors for these two enzymes will shed light on mechanism which cells make choice between cell-cycle arrest apoptosis. It has been reported that ING5, member inhibitor growth (ING) family, involved p53-dependent pathways, but exact role unknown. In this study, we...
β-glucosidases (BGLUs) hydrolyze the β-D-glycosidic bond with retention of anomeric configuration. BGLUs were associated many aspects plant physiological processes, in particular biotic and abiotic stresses through activation phytohormones defense compounds. However, studies on systematic analysis stress- or hormone-responsive BGLU genes are still rare. In this study, total 51 glycoside hydrolase family 1 identified genome model legume Medicago truncatula, they classified into five distinct...
Epimedium pubescens Maxim. is a well-known traditional Chinese medicinal herb with flavonol glycosides as the major pharmaceutically active compounds. UDP-glycosyltransferases (UGTs) are group of enzymes responsible for glycosylation flavonoid glycosides. In this study, genome-wide analysis was performed to identify UGT family genes in E. pubescens. As result, total 339 putative were identified, which represents largest gene known thus far, implying significant expansion All EpUGTs unevenly...
Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor carboxyl terminus Gal4p and inhibits transcription; Gal3p, cytoplasmic transducer that, upon binding galactose adenosine 5'-triphosphate, relieves Gal80p repression. The current model induction relies Gal3p sequestering cytoplasm. However, rapid this system...
MicroRNAs (miRNAs) play important roles in various biological processes and are closely associated with the development of cancer. In fact, aberrant expression miRNAs has been implicated numerous cancers. cervical cancer, miR-203 levels decreased, although cause this remains unclear. study, we investigate molecular mechanisms regulating gene transcription. We identify transcription start site by 5' rapid amplification cDNA ends subsequently promoter region. Promoter analysis revealed that...
The design and construction of mutual interaction models between artificial microsystems living cells have the potential to open a wide range novel applications in biomedical biomimetic technologies. In this study, an form invasion-defense interactions is established community glucose oxidase (GOx)-containing liquid coacervate microdroplets cells, which interact via enzyme-mediated reactive oxygen species (ROS) damage. enzyme-containing microdroplets, formed liquid-liquid phase separation,...
Abstract Background Porcine Parvovirus (PPV) is a Parvovirinae virus that can cause embryonic and fetal loss death mummification in affected pigs. Unlike conventional vaccines, virus-like particles (VLPs) inherit the natural structure of their authentic virions highly immunostimulatory induce strong humoral immune T cell responses with no risk pathogenicity. The production PPV VLPs still challenge based on traditional expression platforms due to low yields high culture costs. Kluyveromyces...
This study explored the application of
Kluyveromyces marxianus is an emerging yeast cell host for diverse products, but multiple-gene expression in K. faces challenges due to limited current knowledge of cis-regulatory elements and insertion loci. Our previous study transferred alien Saccharomyces cerevisiae chromosome I (R1) into marxianus, resulting the creation monochromosomal hybrid KS-R1. All R1 genes were actively transcribed, providing a series loci with varying transcriptional activities. Here, we explore use as novel...
The crosstalk between megakaryocytic lineage cells and the skeletal system has just begun to be explored but remains largely elusive. Using conditional gene knockout mouse models, we demonstrated that loss of Beclin 1 (Becn1), a major regulator mammalian autophagy, exclusively in disrupted autophagy platelets did not compromise megakaryopoiesis or formation function platelets. Unexpectedly, Becn1 deletion male mice led remarkable increase bone mass with improved quality, association decrease...
N-glycosylation is a prevalent post-translational modification in eukaryotes, essential for regulating protein secretion. In Saccharomyces cerevisiae, glycosylation mutants have been shown to enhance the secretion of heterologous glycosylated proteins. However, whether these can also increase non-glycosylated proteins and growth defects associated with mutations be mitigated remains unclear. This study aimed characterize optimize enhanced secretory expression promising yeast host...
Phenotypic plasticity plays a key role in adaptation to fluctuating environments. However, its evolutionary significance remains debated, with conflicting views on whether it is actively maintained by natural selection or neutral consequence of molecular constraints. In this study, we investigate the gene expression yeast populations exposed antibiotic and osmotic stress. Using mutation accumulation (MA) lines separate effects from genetic drift, compare responses (referred as plastic...
Abstract Acute myeloid leukemia (AML), the most common in adults, exhibits immune escape characteristics like solid tumors. The expression of indoleamine 2,3-dioxygenase 1 (IDO1), a well-recognized checkpoint, has been detected AML blast cells and is associated with poor clinical outcome. Although an imbalance B cell subpopulations exists patients’ bone marrow microenvironment, role their interaction IDO1 have yet to be elucidated. Herein, bioinformatic analysis, we found close correlations...