A5100692971- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Immune cells in cancer
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Studies on Chitinases and Chitosanases
- DNA Repair Mechanisms
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Viral Infectious Diseases and Gene Expression in Insects
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Herpesvirus Infections and Treatments
- Phagocytosis and Immune Regulation
- NF-κB Signaling Pathways
Peking University
2016-2025
Huazhong Agricultural University
2012-2025
Shanghai Jiao Tong University
2025
Shanghai Institute of Hematology
2025
Ruijin Hospital
2025
Peking University Third Hospital
2025
King University
2023-2024
Sichuan University
2024
West China Hospital of Sichuan University
2024
Heilongjiang Bayi Agricultural University
2023
Monitoring autophagic flux is important for the analysis of autophagy. Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) a convenient assay monitoring based on different pH stability EGFP and mRFP fluorescent proteins. However, it has been reported that there still weak fluorescence in acidic environments (pH between 4 5) or lysosomes. So possible autolysosomes are labeled with yellow signals (GFP(+)RFP(+) puncta), which results misinterpreting results. Therefore, desirable to choose monomeric...
PDCD10 (programmed cell death 10, TFAR15), a novel protein associated with apoptosis has been recently implicated in mutations Cerebral Cavernous Malformations (CCM). Yeast two-hybrid screening revealed that interacts MST4, member of Ste20-related kinases. This interaction was confirmed by coimmunoprecipitation and colocalization assays mammalian cells. Furthermore, the co-overexpression MST4 promoted proliferation transformation via modulation extracellular signal-regulated kinase (ERK)...
Cytokines are small proteins that have an essential role in the immune and inflammatory responses. The repertoire of cytokines is becoming diverse expanding. Here we report identification characterization a novel cytokine designated as chemokine-like factor 1 (CKLF1). full-length cDNA CKLF1 530bp long single open reading frame encoding 99 amino acid residues. bears no significant similarity to any other known its sequence. Expression can be partly inhibited by interleukin 10 PHA-stimulated...
Cells without Bak and Bax are largely resistant to apoptosis, despite the presence of other key components apoptotic machinery. We screened 7,800 natural compounds found several that could specifically induce caspase activation release cytochrome c (cyto c) in bak(-/-)/bax(-/-) cells. One these was gossypol, a polyphenolic compound naturally cottonseed has been used antifertility trials. but not Bcl-2-interacting molecules, induced cyto loss mitochondrial membrane potential (delta psi m)...
Transition metal copper (Cu) can exist in oxidized or reduced states cells, leading to cytotoxicity cancer cells through oxidative stress. Recently, complexes are emerging as a new class of anticancer compounds. Here, we report that novel complex (HYF127c/Cu) induces stress-dependent cell death cells. Further, transcriptional analysis revealed stress elicits broad changes genes, which autophagy-related genes significantly changed HYF127c/Cu-treated Consistently, autophagy was induced and...
The programmed cell death 5 (PDCD5) protein is a novel related to regulation of apoptosis. In this report, we demonstrate that the level PDCD5 expressed in cells undergoing apoptosis significantly increased compared with normal cells, then translocates rapidly from cytoplasm nucleus cells. appearance nuclei apoptotic precedes externalization phosphatidylserine and fragmentation chromosome DNA. This phenomenon parallel loss mitochondrial membrane potential, independent feature...
Tip60 is a histone acetyltransferase (HAT) involved in the activity and cellular response to DNA damage. Here, we show that programmed cell death 5 (PDCD5), human apoptosis-related protein, binds enhances stability of protein unstressed conditions. The binding amount PDCD5 significantly increased after UV irradiation. Further, HAT Tip60-dependent acetylation both basal UV-induced levels. We also find increases K120 p53 participates p53-dependent expression genes, such as Bax. Moreover,...
Inhibitors of Bcl-XL/Bcl-2 can induce autophagy by releasing the autophagic protein Beclin 1 from its complexes with these proteins. Here we report a novel compound targeting BH3 binding groove Bcl-XL/Bcl-2, Z18, which efficiently induces autophagy-associated cell death in HeLa cells, without apparent apoptosis. Unexpectedly, inhibition and phosphatidylinositol 3-kinase have no obvious effect on Z18-induced implying that it is non-canonical 1-independent autophagy. Meanwhile, accumulation...
Autophagy is mediated by a unique organelle, the autophagosome, which encloses portion of cytoplasm for delivery to lysosome. Phosphatidylinositol 3-phosphate (PtdIns3P) produced class III phosphatidylinositol 3-kinase (PtdIns3K) complex essential canonical autophagosome formation. RAB5A, small GTPase localized early endosomes, has been shown associate with PtdIns3K complex, regulate its activity and promote However, little known about how endosome-localized RAB5A functions complex. Here we...
Residential solid fuels are widely consumed in rural China, contributing to severe household air pollution for many products of incomplete combustion, such as polycyclic aromatic hydrocarbons (PAHs) and their polar derivatives. In this study, concentrations nitrated oxygenated PAH derivatives (nPAHs oPAHs) personal were measured analyzed influencing factors like smoking cooking energy type. Concentrations nPAHs oPAHs kitchens higher than those living rooms outdoor air. Exposure levels by...
Abstract The formation of the autophagosome is controlled by an orderly action ATG proteins. However, how these proteins are recruited to autophagic membranes remain poorly clarified. In this study, we have provided a line evidence confirming that EVA1A (eva-1 homolog A)/TMEM166 (transmembrane protein 166) associated with autophagosomal membrane development. This notion based on dotted structures colocalize ZFYVE1, ATG9, LC3B, ATG16L1, ATG5, STX17, RAB7 and LAMP1, which represent different...
Bovine herpesvirus1 (BoHV-1) is a major bovine pathogen. Despite several vaccines being available to prevent viral infection, outbreaks are frequent and cause important economic consequences worldwide. The development of new antiviral drugs therefore highly desirable. In this context, genome replication represents potential target for therapeutic intervention. BoHV-1 dsDNA molecule whose takes place in the nuclei infected cells mediated by encoded DNA polymerase holoenzyme. Here, we studied...
Programmed Cell Death 5 (PDCD5) is a protein that accelerates apoptosis in different types of cells response to various stimuli and down-regulated many cancer tissues. We hypothesized this study down-regulating PDCD5 can protect the brain from ischemic damage by inhibiting PDCD5-induced apoptotic pathway.One hundred sixty male Sprague-Dawley rats were randomly assigned five groups: Sham surgery (n = 25), MCAO 45), MCAO+rhPDCD5 (RhPDCD5) 30), MCAO+control siRNA MCAO+PDCD5 30). At 24 h...
Self-renewal and differentiation of neural stem cells is essential for embryonic neurogenesis, which associated with cell autophagy. However, the mechanism by autophagy regulates neurogenesis remains undefined. Here, we show that Eva1a/Tmem166, an autophagy-related gene, self-renewal differentiation. Eva1a depletion impaired generation newborn neurons, both in vivo vitro. Conversely, overexpression EVA1A enhanced neuron maturation. Moreover, activated PIK3CA-AKT axis, leading to activation...
MARCH2 (membrane-associated RING-CH protein 2), an E3 ubiquitin ligase, is mainly associated with the vesicle trafficking. In present study, for first time, we demonstrated that negatively regulates autophagy. Our data indicated overexpression of impaired autophagy, as evidenced by attenuated levels LC3B-II and degradation endogenous exogenous autophagic substrates. By contrast, loss expression had opposite effects. vivo experiments demonstrate knockout mediated autophagy results in...
Abstract EVA1A (Eva-1 homologue A) is a novel lysosome and endoplasmic reticulum-associated protein that can regulate cell autophagy apoptosis. Eva1a expressed in the myocardium, but its function myocytes has not yet been investigated. Therefore, we generated inducible, cardiomyocyte-specific knockout mice with an aim to determine role of cardiac remodelling adult heart. Data from experiments showed loss heart increased fibrosis, promoted hypertrophy, led cardiomyopathy death. Further...
Abstract Aims Eva-1 homologue 1 (Eva1a) is a novel protein involved in the regulation of cardiac remodelling and plaque stability, but little known about its role re-endothelialization development atherosclerosis (AS). Thus, present study, we aimed to elucidate function Eva1a AS. Methods results Wire injuries carotid femoral arteries were established Eva1a−/− mice. Eva1a-deficient mice crossed with apolipoprotein E−/− (ApoE−/−) evaluate AS artery injuries. Denudation at 3, 5, 7 days was...