A5100405656- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Genetic diversity and population structure
- Meat and Animal Product Quality
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Animal Disease Management and Epidemiology
- Advanced Drug Delivery Systems
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- RNA Interference and Gene Delivery
- Parasitic Diseases Research and Treatment
- Lung Cancer Treatments and Mutations
- Genetic Mapping and Diversity in Plants and Animals
- Plant biochemistry and biosynthesis
- RNA modifications and cancer
- Biochemical and Structural Characterization
- Inflammasome and immune disorders
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Lipid metabolism and biosynthesis
Harbin Veterinary Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Nanjing Agricultural University
2008-2024
Shenzhen Children's Hospital
2024
Institute of Grassland Research
2024
China Jiliang University
2024
Wuhan University of Science and Technology
2024
Jilin University
2024
Shanxi Medical University
2023
Lanzhou University of Technology
2023
Although several genes involved in mitochondrial function are direct Myc targets, the role of biogenesis has not been directly established. We determined effects ectopic expression or loss on biogenesis. Induction P493-6 cells resulted increased oxygen consumption and mass function. Conversely, compared to wild-type fibroblasts, null rat fibroblasts have diminished decreased number normal mitochondria. Reconstitution partially restored normal-appearing Concordantly, we also observed primary...
Several studies suggest that the open reading frame 3 (ORF3) gene of porcine epidemic diarrhea virus (PEDV) is related to viral infectivity and pathogenicity, but its function remains unknown. Here, we propose a structure model ORF3 protein consisting four TM domains forming tetrameric assembly. can be detected in PEDV‐infected cells it functions as an ion channel both Xenopus laevis oocytes yeast. Mutation analysis showed Tyr170 TM4 important for potassium activity. Furthermore, production...
Coronavirus replication is closely associated with the endoplasmic reticulum (ER), primary cellular organelle for protein synthesis, folding, and modification. ER stress a common consequence in coronavirus-infected cells. However, how virus-induced influences coronavirus pathogenesis remains controversial. Here, we demonstrated that infection alphacoronavirus transmissible gastroenteritis virus (TGEV) induced triggered unfolded response (UPR) vitro vivo, negatively regulated TGEV Although...
Type I interferons (IFN-I) play essential roles in restricting viral infections. Coronavirus infection induces ER stress and the interferon response, which reflects different adaptive cellular processes. An understanding of how coronavirus-elicited is actively involved replication manipulates host IFN-I response has remained elusive. Here, TGEV inhibited miR-30a-5p via sensor IRE1α, led to increased expression negative regulators JAK-STAT signaling cascades, namely, SOCS1 SOCS3. Increased or...
Porcine epidemic diarrhoea virus (PEDV) is an emerging and re-emerging epizootic of swine that causes substantial economic losses to the pig industry in China other countries. The variations virus, its co-infections with enteric viruses, have contributed poor control PEDV infection. In current study, a broad epidemiological investigation was carried out 22 provinces or municipalities during 2015-2018. viruses causing co-infection genetic diversity S1 gene were also analysed. results...
Cytokines are small proteins that have an essential role in the immune and inflammatory responses. The repertoire of cytokines is becoming diverse expanding. Here we report identification characterization a novel cytokine designated as chemokine-like factor 1 (CKLF1). full-length cDNA CKLF1 530bp long single open reading frame encoding 99 amino acid residues. bears no significant similarity to any other known its sequence. Expression can be partly inhibited by interleukin 10 PHA-stimulated...
Severe acute respiratory syndrome (SARS) was caused by a novel virus now known as SARS coronavirus (SARS-CoV). The discovery of SARS-CoV-like viruses in masked palm civets (Paguma larvata) raises the possibility that play role SARS-CoV transmission. To test susceptibility to experimental infection different isolates, 10 were inoculated with two human isolates SARS-CoV, BJ01 (with 29-nucleotide deletion) and GZ01 (without deletion). All animals displayed clinical symptoms, such fever,...
Twenty-six avian infectious bronchitis (IB) viruses (IBV) were isolated from outbreaks in chickens China between 1995 and 2004. They characterized by comparison with twenty-six Chinese reference strains five other IBV strains. IBVs, which mainly nephropathogenic, placed into seven genotypes. Fourteen isolates genotype I, having small evolutionary distances each other. Genotype II included 6 that the 1990s China. III consisted of eight showed close relationship Korean isolates. Another...
In many countries, the method of choice in inspecting meat for Trichinella spiralis infection is artificial digestion. We conducted a study sensitivity digestion recommended by International Commission on Trichinellosis detecting T. larvae and effect modifications some procedures used its sensitivity. As part this, we evaluated effects larval recovery vessels settling, sieve sizes, temperatures at which passed through sieves, using from spiralis-infected mice. observed duration modified 10-g...
To trace evolution of canine parvovirus-2 (CPV-2), a total 201 stool samples were collected from dogs with diarrhea in Heilongjiang province northeast China May 2014 to April 2015. The presence CPV-2 the was determined by PCR amplification VP2 gene (568 bp) CPV-2. results revealed that 95 (47.26%) positive for CPV-2, and they showed 98.8%–100% nucleotide identity 97.6%–100% amino acid identity. Of CPV-2-positive samples, types new2a (Ser297Ala), new2b 2c accounted 64.21%, 21.05%, 14.74%,...
PEDV is a highly contagious enteric coronavirus that causes significant economic losses, and the lack of good in vitro model system major roadblock to an in-depth understanding pathogenesis. Here, we generated porcine intestinal enteroid for infection. Utilizing enteroids, demonstrated infects multiple lineages epithelium preferably ileal enteroids over colonoids prefer respond IFN lambda 1 IFN-α. These events recapitulate occur vivo . This study constitutes first use primary investigate...
Porcine epidemic diarrhea virus (PEDV) is a worldwide-distributed alphacoronavirus, but the pathogenesis of PEDV infection not fully characterized. During infection, type I interferon (IFN) key mediator innate antiviral responses. Most coronaviruses develop some strategy for at least partially circumventing IFN response by limiting production and delaying activation response. However, molecular mechanisms which antagonizes effects have been Especially, how impacts signaling components has...
The emerging Porcine circovirus type 3 (PCV3) is associated with porcine dermatitis and nephropathy syndrome, reproductive failure cardiac multisystemic inflammation. To trace the prevalence evolution of PCV3 in pigs respiratory diseases or digestive China, 616 samples were collected from 21 provinces municipalities China 2015 to 2017. All analysed PCR a cap-gene-based phylogeny. results indicated that positive rate was 12.2% (75/616) at sample level; 24.1% (42/174) farm 10.4% (50/480)...
The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 is profoundly influencing the global healthcare system and people's daily lives. high resource consumption of disease 2019 (COVID-19) resulting in insufficient surveillance coinfection or resurgence other critical epidemics, which public concern. To facilitate evaluation current situation, a microfluidic (MAPnavi) developed for rapid (<40 min) sensitive diagnosis multiple viruses from swab samples fully sealed...
Hmong-Mien (H-M) is a major language family in East Asia, and its speakers distribute primarily southern China Southeast Asia. To date, genetic studies on H-M speaking populations are virtually absent the literature. In this report, we present results of an analysis variations mitochondrial DNA (mtDNA) hypervariable segment 1 (HVS1) region diagnostic variants coding regions 537 individuals sampled from 17 across The showed that haplogroups predominant including B, R9, N9a, M7, account for...
In 2011, outbreaks of viral diarrhea were observed on most swine-breeding farms in the provinces China. The disease is characterized by vomiting, severe diarrhea, and a high mortality rate (82.3%) newborn piglets. clinical appearance was similar to that porcine epidemic virus (PEDV) infection. PEDVs detected samples (feces or small intestines) from farms. order investigate whether there PEDV variant circulating China, we sequenced analyzed complete genome recently identified field strain,...
CH/S is a virulent porcine epidemic diarrhea virus (PEDV) strain and used as the to evaluate protection rates of vaccines against PEDV infection in China. Here, we report complete genome sequence CH/S, which may aid understanding molecular characteristics this strain.