A5101815041- Plant Molecular Biology Research
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Plant Genetic and Mutation Studies
- Soybean genetics and cultivation
- Plant and Fungal Interactions Research
- Plant and fungal interactions
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Horticultural and Viticultural Research
- Synthesis and Characterization of Heterocyclic Compounds
- Genetic and Environmental Crop Studies
- Polysaccharides and Plant Cell Walls
- Ruminant Nutrition and Digestive Physiology
- Agronomic Practices and Intercropping Systems
- Bioenergy crop production and management
- Research on Leishmaniasis Studies
- Tree Root and Stability Studies
Shanghai Ninth People's Hospital
2024-2025
Shanghai Jiao Tong University
2024-2025
Noble Research Institute
2013-2024
Northeast Agricultural University
2022
Eisai (United States)
2020
West Virginia University
2012
BASF (Germany)
2012
Monsanto (India)
2012
Oklahoma State University Institute of Technology
2012
John Innes Centre
2012
Intracellular invasion of root cells is required for the establishment successful endosymbioses in legumes both arbuscular mycorrhizal (AM) fungi and rhizobial bacteria. In interactions a requirement entry activation common signalling pathway that includes five genes to generate calcium oscillations two perception response. Recently, it has been discovered Medicago truncatula, Vapyrin (VPY) gene essential symbiosis. Here, we show by analyses mutants same also colonization nodulation. VPY...
WD40 repeat proteins regulate biosynthesis of anthocyanins, proanthocyanidins (PAs), and mucilage in the seed development trichomes root hairs. We have cloned characterized a protein gene from Medicago truncatula (MtWD40-1) via retrotransposon-tagging approach. Deficiency MtWD40-1 expression blocks accumulation range phenolic compounds, including PAs, epicatechin, other flavonoids, benzoic acids, seed, reduces epicatechin levels without corresponding effects on flavonoids flowers, isoflavone...
RANKL and SCLEROSTIN antibodies have provided a strong effective choice for treating osteoporosis in the past years, which suggested novel molecular target identification therapeutic strategies development are important treatment of osteoporosis. The effect verapamil, drug previously used cardiovascular diseases, on diabetes was due to inhibition TXNIP expression, has also been reported as mice Whether verapamil-inhibited expression is related how it works level worthy be explored....
Stem tissues of tall fescue (Festuca arundinacea Schreb.) were sampled at three elongation stages and reproductive stages. Anatomical analysis showed the deposition guaiacyl (G) syringyl (S) lignin during plant development formation a lignified sclerenchyma ring. A dramatic increase in Klason content was found from stage to stage. Lignin composition analyzed by gas chromatography−mass spectrometry revealed that S S/G ratio increased with stem development, but contents p-hydroxyphenyl (H) G...
Abstract A Tnt1-insertion mutant population of Medicago truncatula ecotype R108 was screened for defects in nodulation and symbiotic nitrogen fixation. Primary screening 9,300 lines yielded 317 with putative nodule development and/or Of these, 230 were rescreened, 156 confirmed defective Mutants sorted into six distinct phenotypic categories: 72 nonnodulating mutants (Nod−), 51 totally ineffective nodules (Nod+ Fix−), 17 partially Fix+/−), 27 emergence, elongation, fixation (Nod+/− one...
Summary M edicago truncatula is one of the model species for legume studies. In an effort to develop genetics resources, > 21 700 T nt1 retrotransposon insertion lines have been generated. To facilitate fast‐growing needs in functional genomics, two reverse approaches established: web‐based database searching and PCR ‐based screening. More than 840 genes screened using approach over past 6 yr identify mutants these genes. Overall, c . 84% (705 genes) success rate was achieved identifying...
Summary The CUP‐SHAPED COTYLEDON ( CUC )/ NO APICAL MERISTEM NAM ) family of genes control boundary formation and lateral organ separation, which is critical for proper leaf flower patterning. However, most downstream targets CUC/NAM remain unclear. In a forward screen the tobacco retrotransposon1 Tnt1 insertion population in Medicago truncatula , we isolated weak allele no‐apical‐meristem mutant mtnam‐2 . Meanwhile, regenerated mature plant from null mtnam‐1 These materials allowed us to...
Abstract Leaf shape elaboration and organ separation are critical for plant morphogenesis. We characterized the developmental roles of LOBED LEAFLET1 by analyzing a recessive mutant in model legume Medicago truncatula. An ortholog Arabidopsis thaliana ARGONAUTE7 (AGO7), Mt-AGO7/LOBED LEAFLET1, is required biogenesis trans-acting short interfering RNA (ta-siRNA) to negatively regulate expression AUXIN RESPONSE FACTORs M. Loss function AGO7 results pleiotropic phenotypes different organs. The...
Most legume plants can form nodules, specialized lateral organs that on roots, and house nitrogen-fixing bacteria collectively called rhizobia. The uptake of the phytohormone auxin into cells is known to be crucial for development roots. To test role influx in nodulation we used inhibitors 1-naphthoxyacetic acid (1-NOA) 2-NOA, which found reduced Medicago truncatula. This suggested possible involvement AUX/LAX family transporters nodulation. Gene expression studies identified MtLAX2, a...
Development of inflorescence architecture is controlled by genetic regulatory networks. TERMINAL FLOWER1 (TFL1), APETALA1 (AP1), LEAFY (LFY), and FRUITFULL (FUL) are core regulators for development. To understand the regulation compound development, we characterized mutants corresponding orthologous genes, MtTFL1, MtAP1, SINGLE LEAFLET1 (SGL1), MtFULc, in Medicago truncatula, analyzed expression patterns these genes. Results indicate that SGL1 play specific roles identity determination...
Class I KNOTTED-like homeobox (KNOXI) genes are critical for the maintenance of shoot apical meristem. The expression domain KNOXI is regulated by ASYMMETRIC LEAVES1/ROUGHSHEATH2/PHANTASTICA (ARP) genes, which associated with leaf morphology. In inverted repeat-lacking clade (IRLC) Fabaceae, orthologs LEAFY (LFY) function in place to regulate compound development. Here, we characterized loss-of-function mutants ARP (PHAN) and SHOOTMERISTEMLESS (STM)- BREVIPEDICELLUS (BP)-like model IRLC...
Brassinosteroids are phytohormones involved in plant development and physiological processes. Insensitive 1 (BRI1) is required for BR perception initiation of subsequent signal transduction Arabidopsis. In this study, the orthologue BRI1 model legume species Medicago truncatula, MtBRI1, was identified characterised. Three allelic Tnt1 insertion mutants, mtbri1-1, mtbri1-2, mtbri1-3, were obtained from M. truncatula population. mtbri1 mutants displayed characteristic bri1 mutant phenotypes:...
Transfer (T)-DNA insertions in mutants isolated from forward genetic screens are typically identified through thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR), inverse PCR, or plasmid rescue. Despite the popularity and success of these methods, they have limited capabilities, particularly situations which T-DNA is truncated. Here, we present a next generation sequencing (NGS)-based platform to facilitate identification complete truncated insertions. Our method enables...
Cinnamoyl co-enzyme A reductase (CCR), one of the key enzymes involved in biosynthesis monolignols, has been thought to catalyze conversion several cinnamoyl-CoA esters their respective cinnamaldehydes. However, it is unclear which ester metabolized for monolignol biosynthesis. xylem-specific CCR cDNA was cloned from aspen (Populus tremuloides) developing xylem tissue. The recombinant protein produced through an Escherichia coli expression system and purified electrophoretic homogeneity....
Legumes are important to humans by providing food, feed and raw materials for industrial utilizations. Some legumes, such as alfalfa, potential bioenergy crops due their high biomass productivity. Global transcriptional profiling has been successfully used identify genes regulatory pathways in secondary cell wall thickening Arabidopsis, but transcriptome data is lacking legumes.A systematic microarray assay through-put real time PCR analysis of development were performed along stem...
Abstract Regulation of floral transition and inflorescence development is crucial for plant reproductive success. FLOWERING LOCUS T (FT) one the central players in flowering genetic regulatory network, whereas D (FD), an interactor FT TERMINAL FLOWER 1 (TFL1), plays significant roles both development. Here we show networks Medicago truncatula by characterizing MtFTa1 MtFDa their interactions with key meristem (IM) regulators. Both promote flowering; double mutant mtfda mtfta1 does not...
Tall fescue (Festuca arundinacea Schreb) is a major cool season forage and turf grass species grown in the temperate regions of world. In this paper we report generation tall expressed sequence tag (EST) database developed from nine cDNA libraries representing tissues different plant organs, developmental stages, abiotic stress factors. The results inter-library library-specific silico expression analyses these ESTs are also reported.A total 41,516 were generated conditions. Festuca Gene...
Deletion mutagenesis such as fast neutron bombardment (FNB) has been widely used for forward and reverse genetics studies in functional genomics. Traditionally, the time-consuming map-based cloning is to locate causal deletions deletion mutants. In recent years, comparative genomic hybridization (CGH) speed up scale lesion identification process However, limitations of low accuracy sensitivity small CGH approach are apparent. With next generation sequencing (NGS) becoming affordable most...
Abstract Background MicroRNAs (miRNAs) and other epigenetic modifications play fundamental roles in all eukaryotic biological processes. DNA damage repair is a key process for maintaining the genomic integrity of different organisms exposed to diverse stresses. However, reaction miRNAs unclear. Results In this study, we found that simultaneous mutation zinc finger 3′-phosphoesterase ( ZDP ) AP endonuclease 2 APE2 ), two genes overlapping active demethylation base excision (BER), led...