A5009425615- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant responses to water stress
- Plant tissue culture and regeneration
- Traditional Chinese Medicine Analysis
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Plant Virus Research Studies
- Ginseng Biological Effects and Applications
- Plant and Fungal Interactions Research
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Coenzyme Q10 studies and effects
- Nephrotoxicity and Medicinal Plants
- Microbial Natural Products and Biosynthesis
- Natural product bioactivities and synthesis
- Plant Disease Management Techniques
- Plant nutrient uptake and metabolism
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Ministry of Education of the People's Republic of China
2023-2025
State Administration of Traditional Chinese Medicine of the People's Republic of China
2023-2024
Institute of Medicinal Plant Development
2016
Shanxi Normal University
2014
North Carolina State University
2004-2011
Peking Union Medical College Hospital
2010
Institute of Botany
2000
Chinese Academy of Sciences
1999-2000
Beijing Botanical Garden
2000
Abstract MicroRNAs (miRNAs) are small, noncoding RNAs that can play crucial regulatory roles in eukaryotes by targeting mRNAs for silencing. To test whether miRNAs the regulation of wood development tree species, we isolated small from developing xylem Populus trichocarpa stems and cloned 22 miRNAs. They founding members 21 miRNA gene families 48 sequences, represented 98 loci genome. A majority these were predicted to target developmental- stress/defense-related genes possible functions...
MicroRNAs (miRNAs), a group of small non-coding RNAs, have recently become the subject intense study. They are class post-transcriptional negative regulators playing vital roles in plant development and growth. However, little is known about their regulatory responses trees to stressful environments incurred over long-term Here, we report cloning RNAs from abiotic stressed tissues Populus trichocarpa (Ptc) identification 68 putative miRNA sequences that can be classified into 27 families...
Phenylpropanoids are a large class of plant secondary metabolites derived from aromatic amino acids phenylalanine in most plants or tyrosine partial monocots. It mainly includes flavonoids, monolignols, phenolic acids, stilbenes, and coumarins. widely distributed the kingdom play vital roles development by acting as essential components cell walls, protectants against high light UV radiation, phytoalexins herbivores pathogens, floral pigments to mediate plant–pollinator interactions. In...
Laccases, as early 1959, were proposed to catalyze the oxidative polymerization of monolignols. Genetic evidence in support this hypothesis has been elusive due functional redundancy laccase genes. An Arabidopsis double mutant demonstrated involvement laccases lignin biosynthesis. We previously identified a subset genes be targets microRNA (miRNA) ptr-miR397a Populus trichocarpa . To elucidate roles and its targets, we characterized gene family 49 models, which 29 predicted ptr-miR397a....
Terpenoids are the largest class of plant secondary metabolites and have attracted widespread interest.Salvia miltiorrhiza, belonging to most widely distributed genus in mint family, is a model medicinal with great economic value.Diterpenoid tanshinones major lipophilic bioactive components S. miltiorrhiza.Systematic analysis genes involved terpenoid biosynthesis has not been reported date.Searching recently available working draft miltiorrhiza genome, 40 biosynthesis-related were...
MicroRNAs (miRNAs) are endogenous small RNAs that can have large-scale regulatory effects on development and stress responses in plants. The endemic rust fungus Cronartium quercuum f. sp. fusiforme causes fusiform disease pines, resulting the of spindle-shaped galls (cankers) branches or stems. This is most destructive pines southern USA. To test whether miRNAs play roles gall development, we cloned identified 26 from stem xylem loblolly pine (Pinus taeda), which belong to four conserved...
The modification or degradation of RNAs including miRNAs may play vital roles in regulating RNA functions. polyadenylation- and exosome-mediated decay is involved the plant primary miRNA processing intermediates. However, levels are not affected by exosome depletion. Here, we report cloning a large number 5′ and/or 3′ truncated versions known from various tissues Populus trichocarpa (black cottonwood). It suggests that be degraded through either to exonucleolytic digestion. We also show...
MYB is the largest plant transcription factor gene family playing vital roles in growth and development. However, it has not been systematically studied Salvia miltiorrhiza, an economically important medicinal plant. Here we report genome-wide identification characterization of 110 R2R3-MYBs, subfamily MYBs S. miltiorrhiza. The domain other motifs SmMYBs are largely conserved with Arabidopsis AtMYBs, whereas divergence AtMYBs also exists, suggesting conservation diversity MYBs. may be...
WRKY proteins comprise a large family of transcription factors and play important regulatory roles in plant development defense response. The gene Salvia miltiorrhiza has not been characterized.A total 61 SmWRKYs were cloned from S. miltiorrhiza. Multiple sequence alignment showed that could be classified into 3 groups 8 subgroups. Sequence features, the domain other motifs are largely conserved with Arabidopsis AtWRKYs. Each group domains contains characteristic sequences, group-specific...
Abstract Salvia miltiorrhiza is well known for its clinical practice in treating heart and cardiovascular diseases. Its roots, used traditional Chinese medicine materials, are usually brick-red due to accumulation of red pigments, such as tanshinone IIA I. Here we report a S. line (shh) with orange roots. Compared the roots normal plants, contents tanshinones single bond at C-15,16 were increased, whereas those double significantly decreased shh. We assembled high-quality chromosome-level...
SUMMARY Prunella vulgaris is one of the bestselling and widely used medicinal herbs. It recorded as an ace medicine for cleansing protecting liver in Chinese Pharmacopoeia has been main constitutions many herbal tea formulas China centuries. also a traditional folk Europe other countries Asia. Pentacyclic triterpenoids are major class bioactive compounds produced P. . However, their biosynthetic mechanism remains to be elucidated. Here, we report chromosome‐level reference genome using...
Aporphine alkaloids are a large group of natural compounds with extensive pharmaceutical application prospects. The biosynthesis aporphine has been paid attentions in the past decades. Here, we determined contents four 1-benzylisoquinoline and five root, stem, leaf, flower Aristolochia contorta Bunge, which belongs to magnoliids. Two CYP80 enzymes were identified characterized from A. contorta. Both them catalyze unusual C-C phenol coupling reactions directly form alkaloid skeleton....
We report here the Agrobacterium -mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are explants most amenable to transformation. For efficient system, approximately 40% infected with pBI121-containing tumefaciens C58 produced transgenic calli, as confirmed by β-glucuronidase (GUS) staining. The regeneration efficiency...
SPLs, a family of transcription factors specific to plants, play vital roles in plant growth and development through regulation various physiological biochemical processes. Although Populus trichocarpa is model forest tree, the PtSPL gene has not been systematically studied. Here we report identification 28 full-length PtSPLs, which distribute on 14 P. chromosomes. Based phylogenetic relationships SPLs Arabidopsis, can be classified into 6 groups. Each group contains at least an AtSPL. The...
Abstract Background Digitalis purpurea is an important ornamental and medicinal plant. There considerable interest in exploring its transcriptome. Results Through high-throughput 454 sequencing subsequent assembly, we obtained 23532 genes, of which 15626 encode conserved proteins. We determined 140 unigenes to be candidates involved cardiac glycoside biosynthesis. It could grouped into 30 families, 29 were identified for the first time D. . 2660 mRNA-like npcRNA (mlncRNA) candidates,...
Abstract Background Small non-coding RNAs (sRNAs) play key roles in plant development, growth and responses to biotic abiotic stresses. At least four classes of sRNAs have been well characterized plants, including repeat-associated siRNAs (rasiRNAs), microRNAs (miRNAs), trans -acting (tasiRNAs) natural antisense transcript-derived siRNAs. Chinese fir ( Cunninghamia lanceolata ) is one the most important coniferous evergreen tree species China. No sRNA from has described date. Results To...
Summary C hinese fir ( unninghamia lanceolata ), a commercially important tree for the timber and pulp industry, is widely distributed in southern hina northern V ietnam, but its large complex genome has hindered development of genomic resources. Few efforts have focused on analysis modulation transcriptional networks vascular cambium during transition from active growth to dormancy conifers. Here, we used I llumina sequencing analyze global transcriptome alterations at different stages fir....
Abstract SQUAMOSA promoter binding protein‐likes (SPLs) are plant‐specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs Salvia miltiorrhiza (Danshen), a significant medicinal widely used Traditional Chinese medicine (TCM) for >1,700 years an emerging model TCM studies. Through genome‐wide identification subsequent molecular cloning, we identified total 15 SmSPLs with divergent sequence features, gene structures,...
Flavonoids are a class of important secondary metabolites with broad spectrum pharmacological functions. Salvia miltiorrhiza Bunge (Danshen) is well-known traditional Chinese medicinal herb diversity flavonoids. However, flavonoid biosynthetic enzyme genes have not been systematically and comprehensively analyzed in S. miltiorrhiza. Through genome-wide prediction molecular cloning, twenty six biosynthesis-related gene candidates were identified, which novel. They belong to nine families...
Fungi have evolved powerful genomic and chemical defense systems to protect themselves against genetic destabilization other organisms. However, the precise molecular basis involved in fungal remain largely unknown Basidiomycetes. Here complete genome sequence, as well DNA methylation patterns small RNA transcriptomes, was analyzed provide a holistic overview of secondary metabolism processes model medicinal fungus, Ganoderma sinense. We reported 48.96 Mb sequence G. sinense, consisting 12...
Salvia miltiorrhiza is one of the most well known species in genus Lamiaceae with great economic, academic and medicinal value. It was recorded as a traditional Chinese medicine material first about second century BC later many other ancient books. can be clinically used alone or mixed herbs to treat heart cardiovascular diseases beneficial for management diseases. In last 30 years, S. has been studied intensively model system plant biology. With available whole genome sequence four lines...
Abstract Tanshinones and phenolic acids are two major classes of bioactive compounds in Salvia miltiorrhiza. Revealing the regulatory mechanism their biosynthesis is crucial for quality improvement S. miltiorrhiza medicinal materials. Here we demonstrated that Smi-miR858a–Smi-miR858c, a miRNA family previously known to regulate flavonoid biosynthesis, also played critical roles tanshinone acid Overexpression Smi-miR858a plants caused significant growth retardation reduction. Computational...