A5101701338- Plant biochemistry and biosynthesis
- Ginseng Biological Effects and Applications
- Plant Gene Expression Analysis
- Natural product bioactivities and synthesis
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Genetic diversity and population structure
- Plant Reproductive Biology
- Molecular Biology Techniques and Applications
- Plant and Fungal Interactions Research
- CRISPR and Genetic Engineering
- Traditional Chinese Medicine Analysis
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Alkaloids: synthesis and pharmacology
- Pharmacological Effects of Natural Compounds
- Plant-Microbe Interactions and Immunity
- Vector-Borne Animal Diseases
- Plant and Fungal Species Descriptions
- Medicinal Plants and Bioactive Compounds
- Medicinal Plant Pharmacodynamics Research
- Cholinesterase and Neurodegenerative Diseases
- Mycorrhizal Fungi and Plant Interactions
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2025
Gansu Agricultural University
2022-2025
Academy of Medical Sciences
2012-2025
Institute of Medicinal Plant Development
2019-2025
Harbin Veterinary Research Institute
2022-2024
Chinese Academy of Agricultural Sciences
2022-2024
Fujian Agriculture and Forestry University
2024
State Administration of Traditional Chinese Medicine of the People's Republic of China
2019-2024
Ministry of Education of the People's Republic of China
2024
Shandong University
2022-2023
Salvia miltiorrhiza is an important medicinal plant with great economic and value. The complete chloroplast (cp) genome sequence of miltiorrhiza, the first sequenced member Lamiaceae family, reported here. 151,328 bp in length exhibits a typical quadripartite structure large (LSC, 82,695 bp) small (SSC, 17,555 single-copy regions, separated by pair inverted repeats (IRs, 25,539 bp). It contains 114 unique genes, including 80 protein-coding 30 tRNAs four rRNAs. structure, gene order, GC...
Abstract Background American ginseng ( Panax quinquefolius L.) is one of the most widely used herbal remedies in world. Its major bioactive constituents are triterpene saponins known as ginsenosides. However, little about ginsenoside biosynthesis ginseng, especially late steps pathway. Results In this study, a one-quarter 454 sequencing run produced 209,747 high-quality reads with an average sequence length 427 bases. De novo assembly generated 31,088 unique sequences containing 16,592...
Abstract Background Panax notoginseng (Burk) F.H. Chen is important medicinal plant of the Araliacease family. Triterpene saponins are bioactive constituents in P. . However, available genomic information regarding this limited. Moreover, details triterpene saponin biosynthesis species largely unknown. Results Using 454 pyrosequencing technology, a one-quarter GS FLX titanium run resulted 188,185 reads with an average length 410 bases for root. These were processed and assembled by De Novo...
Panax ginseng C. A. Meyer is one of the most widely used medicinal plants. Complete genome information for this species remains unavailable due to its large size. At present, analysis expressed sequence tags still powerful tool large-scale gene discovery. The global from P. tissues, especially those isolated stems, leaves and flowers, are limited, hindering in-depth study ginseng.
Salvia miltiorrhiza is a Chinese herb with significant pharmacologic effects because of the bioactive compounds tanshinones and phenolic acids. Methyl jasmonate ( MeJA ) has been used as an effective elicitor to enhance production these compounds. However, molecular mechanism ‐mediated tanshinone salvianolic acid biosynthesis remains unclear. The transcriptional profiles S. leaves at 12 h T12 after elicitation mock‐treated T0 were generated using Illumina deep RNA sequencing ‐seq) strategy...
Summary A circular consensus sequencing ( CCS ) strategy involving single molecule, real‐time SMRT DNA technology was applied to de novo assembly and nucleotide polymorphism SNP detection of chloroplast genomes. Chloroplast purified from enriched chloroplasts pooled individuals construct a shotgun library for each species. The reactions were performed on PacBio RS platform. sub‐reads generated polymerase reads that passed the native dumbbell‐shaped templates multiple times. complete genome...
Abstract Background Glycyrrhiza uralensis is one of the most popular medicinal plants in world and also widely used flavoring food tobacco. Due to limited genomic transcriptomic data, biosynthetic pathway glycyrrhizin, major bioactive compound G. , currently unclear. Identification candidate genes involved glycyrrhizin will significantly contribute understanding chemistry this compound. Results We 454 GS FLX platform Titanium regents produce a substantial expressed sequence tag (EST) dataset...
Abstract Background Camptotheca acuminata is a Nyssaceae plant, often called the "happy tree", which indigenous in Southern China. C. produces terpenoid indole alkaloid, camptothecin (CPT), exhibits clinical effects various cancer treatments. Despite its importance, little known about transcriptome of and mechanism CPT biosynthesis, as only few nucleotide sequences are included GenBank database. Results From constructed cDNA library young leaves, total 30,358 unigenes, with an average length...
Fungi have evolved powerful genomic and chemical defense systems to protect themselves against genetic destabilization other organisms. However, the precise molecular basis involved in fungal remain largely unknown Basidiomycetes. Here complete genome sequence, as well DNA methylation patterns small RNA transcriptomes, was analyzed provide a holistic overview of secondary metabolism processes model medicinal fungus, Ganoderma sinense. We reported 48.96 Mb sequence G. sinense, consisting 12...
Taxus cuspidata is well known worldwide for its ability to produce Taxol, one of the top-selling natural anticancer drugs. However, current Taxol production cannot match increasing needs market, and novel strategies should be considered increase supply Taxol. Since biosynthetic mechanism remains largely unknown, elucidating this pathway in detail will very helpful exploring alternative methods production.Here, we sequenced transcriptomes with next-generation sequencing (NGS) third-generation...
Abstract The anthocyanin content in apple skin determines its red coloration, as seen a Fuji mutant. Comparative RNA-seq analysis was performed to determine differentially expressed genes at different fruit development stages between the wild-type and color A novel R2R3-MYB transcription factor, MdMYB90-like, uncovered key regulatory gene for enhanced coloration expression of MdMYB90-like 21.3 times higher regulates biosynthesis directly through activation indirectly other factors that...
There is accumulating evidence that mitochondrial dysfunction associated with the contribution of diabetes to Alzheimer disease (AD) progression. Neuronal proteins are found in plasma neuronal-derived exosomes (NDEs) at levels reflect those brain neurons. Here, we tested performance NDEs predict cognitive decline and injury participants diabetes. The study type 2 mellitus (T2DM) included 41 cognitively normal control subjects, 97 individuals mild impairment (MCI) (68 stable MCI; 29...
Abstract Tanshinones and phenolic acids are two major classes of bioactive compounds in Salvia miltiorrhiza. Revealing the regulatory mechanism their biosynthesis is crucial for quality improvement S. miltiorrhiza medicinal materials. Here we demonstrated that Smi-miR858a–Smi-miR858c, a miRNA family previously known to regulate flavonoid biosynthesis, also played critical roles tanshinone acid Overexpression Smi-miR858a plants caused significant growth retardation reduction. Computational...