A5036173674- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Mesenchymal stem cell research
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Cancer-related molecular mechanisms research
- Crystallization and Solubility Studies
- TGF-β signaling in diseases
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- Graphene and Nanomaterials Applications
- Transgenic Plants and Applications
- Plant Reproductive Biology
- RNA Interference and Gene Delivery
- Insect Resistance and Genetics
- dental development and anomalies
- Viral Infectious Diseases and Gene Expression in Insects
- Crystallography and molecular interactions
- Plant Virus Research Studies
- Chronic Kidney Disease and Diabetes
- CRISPR and Genetic Engineering
- Plant Stress Responses and Tolerance
- Lanthanide and Transition Metal Complexes
- Plant Disease Resistance and Genetics
Nanjing Agricultural University
2014-2025
Shanghai University of Electric Power
2025
Nanjing Medical University
2025
Southern Medical University
2020-2025
Jiangsu Province Hospital
2025
Chongqing Medical University
2016-2024
Stomatology Hospital
2023-2024
Sun Yat-sen University
2023-2024
Children's Hospital of Chongqing Medical University
2024
Zhejiang Ocean University
2024
Background/Aims: Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into several lineages including bone. Successful bone formation requires osteogenesis and angiogenesis coupling of MSCs. Here, we investigate if simultaneous activation BMP9 Notch signaling yields effective osteogenesis-angiogenesis in Methods: Recently-characterized immortalized mouse adipose-derived (iMADs) were used as MSC source. Transgenes BMP9, NICD dnNotch1 expressed by adenoviral...
With rapid advances in understanding molecular pathogenesis of human diseases the era genome sciences and systems biology, it is anticipated that increasing numbers therapeutic genes or targets will become available for targeted therapies. Despite numerous setbacks, efficacious gene and/or cell-based therapies still hold great promise to revolutionize clinical management diseases. It wildly recognized poor delivery limiting factor most vivo There has been a long-lasting interest using viral...
Mesenchymal stem cells (MSCs) are multipotent progenitor that can undergo self-renewal and differentiate into multiple lineages. Osteogenic differentiation from MSCs is a well-orchestrated process regulated by signaling pathways. We previously demonstrated BMP9 one of the most potent osteogenic factors. However, molecular mechanism through which governs osteoblastic remains to be fully understood. Increasing evidence indicates noncoding RNAs (ncRNAs) may play important regulatory roles in...
The liver provides vital metabolic, exocrine and endocrine functions in the body as such pathological conditions of lead to high morbidity mortality. is highly regenerative contains facultative stem cells that become activated during injury replicate fully recover mass function. Canonical Wnt/β-catenin signaling plays an important role regulating proliferation differentiation progenitor regeneration. However, possible roles noncanonical Wnts development regeneration remain undefined. We...
Abstract The relationship of obesity and osteoporosis has been widely studied over the past years. However, implications for bone health remain controversial, underlying molecular mechanism is not yet fully understood. This study demonstrated that high‐fat diet‐induced leads to significantly decreased volume/tissue volume (BV/TV), trabecular number (Tb.N), cortical thickness (Ct.Th) male rat femur after mechanical loading effects body weight were controlled. HFD‐induced obese rats exhibited...
Lamotrigine (LAM) is a widely used first-line anti-epileptic drug with limitations due to its poor water solubility and side effects. Over the years, numerous attempts modify LAM's physicochemical properties...
Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into multiple lineages including osteoblastic lineage. Osteogenic differentiation of MSCs is a cascade recapitulates most, if not all, the molecular events occurring during embryonic skeletal development, which regulated by numerous signaling pathways bone morphogenetic proteins (BMPs). Through comprehensive analysis osteogenic activity, we previously demonstrated BMP9 most potent BMP for inducing formation from...
Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic progenitor that can undergo self-renewal and differentiate into multi-lineages. Bone marrow stromal (BMSCs) represent one of the most commonly-used MSCs. In order to overcome technical challenge maintaining primary BMSCs in long-term culture, here we seek establish reversibly immortalized mouse (imBMSCs). By exploiting CRISPR/Cas9-based homology-directed-repair (HDR) mechanism, target SV40T Rosa26 locus efficiently immortalize...
Effective bone tissue engineering is important to overcome the unmet clinical challenges as more than 1.6 million grafts are done annually in United States. Successful needs minimally three critical constituents: osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds. Osteogenic progenitors derived from multipotent mesenchymal stem cells (MSCs), which can be prepared numerous sources, including adipose tissue. We previously showed that BMP9 most BMP induces...
Abstract The anthocyanin content in apple skin determines its red coloration, as seen a Fuji mutant. Comparative RNA-seq analysis was performed to determine differentially expressed genes at different fruit development stages between the wild-type and color A novel R2R3-MYB transcription factor, MdMYB90-like, uncovered key regulatory gene for enhanced coloration expression of MdMYB90-like 21.3 times higher regulates biosynthesis directly through activation indirectly other factors that...
Head and neck squamous cell carcinoma (HNSCC) is one of the most common aggressive types human cancers worldwide. Nearly a half HNSCC patients experience recurrence within five years treatment develop resistance to chemotherapy. Thus, there an urgent clinical need safe novel anticancer therapies for HNSCC. Here, we investigate possibility repurposing anthelmintic drug mebendazole (MBZ) as anti-HNSCC agent. Using two commonly-used lines CAL27 SCC15, demonstrate MBZ exerts more potent...
While recombinant adenoviruses are among the most widely-used gene delivery vectors and usually propagated in HEK-293 cells, generating remains time-consuming labor-intense. We sought to develop a rapid adenovirus production amplification (RAPA) line by assessing human Ad5 genes (E1A, E1B19K/55K, pTP, DBP, DNA Pol) OCT1 for their contributions production.Stable transgene expression 293T cells was accomplished using piggyBac system. Transgene determined qPCR. Adenoviral assessed with...
Tooth is a complex hard tissue organ and consists of multiple cell types that are regulated by important signaling pathways such as Wnt BMP signaling. Serious injuries and/or loss tooth or periodontal tissues may significantly impact aesthetic appearance, essential oral functions the quality life. Regenerative dentistry holds great promise in treating oral/dental disorders. The past decade has witnessed rapid expansion our understanding biological features dental stem cells, along with...
Abstract The Alternaria alternata apple pathotype adversely affects ( Malus domestica Borkh.) cultivation. However, the molecular mechanisms underlying enhanced resistance to this pathogen in remain poorly understood. We have previously reported that MdWRKY75 expression is upregulated by A. infection ‘Sushuai’ apples. In study, we discovered overexpression of MdWRKY75e increased transgenic lines infection, whereas silencing gene susceptibility infection. Furthermore, found directly binds...
In this study, the crystal structures of 1:1 theophylline–ferulic acid cocrystal polymorphs are reported for first time. Interestingly, both forms crystallize in same space group but with a different number molecules asymmetric unit (form I = 2; form II 4). The two show distinct morphology, demonstrating prism and rod single crystals, respectively. Theoretical calculations performed to predict their morphology thermodynamic stability > II). prediction result agrees well measured...
Abstract Human mesenchymal stem cells (MSCs) are a heterogeneous subset of nonhematopoietic multipotent stromal and can differentiate into mesodermal lineage, such as adipocytes, osteocytes, chondrocytes, well ectodermal endodermal lineages. umbilical cord (UC) is one the most promising sources MSCs. However, molecular cellular characteristics UC‐derived MSCs (UC‐MSCs) require extensive investigations, which hampered by limited lifespan diminished potency over passages. Here, we used...
Abstract The cranial suture complex is a heterogeneous tissue consisting of osteogenic progenitor cells and mesenchymal stem ( MSC s) from bone marrow mesenchyme. fusion sutures highly coordinated tightly regulated process during development. Craniosynostosis congenital malformation caused by premature sutures. While the derived should prove valuable for studying molecular mechanisms underlying development pathogenic fusion, primary human progenitors (SuPs) have limited life span gradually...
Ovarian cancer is the most lethal gynecologic malignancy, and there an unmet clinical need to develop new therapies. Although showing promising anticancer activity, Niclosamide may not be used as a monotherapy. We seek investigate whether inhibiting IGF signaling potentiates Niclosamide's efficacy in human ovarian cells.Cell proliferation migration are assessed. Cell cycle progression apoptosis analyzed by flow cytometry. Inhibition of accomplished adenovirus-mediated expression siRNAs...
Abstract During extreme meteorological events, the output of wind farms may undergo noticeable fluctuations within short timeframes, leading to power ramp events (WPREs), which can result in severe disruptions system, potentially causing widespread outages. However, limited studies have dedicated addressing adverse impacts WPREs on system. Moreover, few articles consider influence correlations among multiple WPREs. This paper proposes an improved multi‐parameter segmentation algorithm for...
Abstract Severe fever with thrombocytopenia syndrome is a life-threatening condition that has been the focus of attention in recent years. It primarily caused by uncontrolled replication novel Bunyavirus and an intense pro-inflammatory response. NETosis form cell death initiated neutrophils, involving formation neutrophil extracellular traps. These NETs are composed DNA fibers or nuclear chromatin trap cytoplasmic granule proteins histones meshwork to capture eliminate pathogens.Our...
MicroRNA (miRNA)-mediated post-transcriptional regulation plays a fundamental role in various plant physiological processes, including responses to pathogens. MicroRNA168 has been implicated as an essential factor of miRNA pathways by targeting ARGONAUTE1 (AGO1), the core component RNA-induced silencing complex (RISC). A fluctuation AGO1 expression influences plant–pathogen interactions, and homeostasis miR168 accumulation is maintained complicated feedback regulatory loop. In this study,...