A5016306243- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- RNA modifications and cancer
- Effects and risks of endocrine disrupting chemicals
- RNA Interference and Gene Delivery
- Carcinogens and Genotoxicity Assessment
- Mitochondrial Function and Pathology
- bioluminescence and chemiluminescence research
- Dermatological and COVID-19 studies
- Epigenetics and DNA Methylation
- Parvovirus B19 Infection Studies
- Tuberculosis Research and Epidemiology
- S100 Proteins and Annexins
- Nuclear Receptors and Signaling
- Tissue Engineering and Regenerative Medicine
- Ultrasound and Hyperthermia Applications
- Wnt/β-catenin signaling in development and cancer
- High voltage insulation and dielectric phenomena
- Immune Response and Inflammation
- MicroRNA in disease regulation
- Pediatric health and respiratory diseases
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Hedgehog Signaling Pathway Studies
Guizhou University
2024
Chongqing Medical University
2014-2023
Children's Hospital of Chongqing Medical University
2014-2023
China Three Gorges University
2020-2023
China International Science and Technology Cooperation
2016-2022
The Affiliated Yongchuan Hospital of Chongqing Medical University
2017-2022
China Energy Engineering Corporation (China)
2020
University of Chicago Medical Center
2015-2018
Molecular Oncology (United States)
2015-2018
Central China Normal University
2011-2012
Background/Aims: Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into several lineages including bone. Successful bone formation requires osteogenesis and angiogenesis coupling of MSCs. Here, we investigate if simultaneous activation BMP9 Notch signaling yields effective osteogenesis-angiogenesis in Methods: Recently-characterized immortalized mouse adipose-derived (iMADs) were used as MSC source. Transgenes BMP9, NICD dnNotch1 expressed by adenoviral...
With rapid advances in understanding molecular pathogenesis of human diseases the era genome sciences and systems biology, it is anticipated that increasing numbers therapeutic genes or targets will become available for targeted therapies. Despite numerous setbacks, efficacious gene and/or cell-based therapies still hold great promise to revolutionize clinical management diseases. It wildly recognized poor delivery limiting factor most vivo There has been a long-lasting interest using viral...
The canonical WNT/β-catenin signaling pathway governs a myriad of biological processes underlying the development and maintenance adult tissue homeostasis, including regulation stem cell self-renewal, proliferation, differentiation, apoptosis. WNTs are secreted lipid-modified glycoproteins that act as short-range ligands to activate receptor-mediated pathways. hallmark is activation β-catenin-mediated transcriptional activity. Canonical control β-catenin dynamics cytoplasmic level tightly...
Mesenchymal stem cells (MSCs) are multipotent progenitor that can undergo self-renewal and differentiate into multiple lineages. Osteogenic differentiation from MSCs is a well-orchestrated process regulated by signaling pathways. We previously demonstrated BMP9 one of the most potent osteogenic factors. However, molecular mechanism through which governs osteoblastic remains to be fully understood. Increasing evidence indicates noncoding RNAs (ncRNAs) may play important regulatory roles in...
The liver provides vital metabolic, exocrine and endocrine functions in the body as such pathological conditions of lead to high morbidity mortality. is highly regenerative contains facultative stem cells that become activated during injury replicate fully recover mass function. Canonical Wnt/β-catenin signaling plays an important role regulating proliferation differentiation progenitor regeneration. However, possible roles noncanonical Wnts development regeneration remain undefined. We...
This study investigated epigenetic (specifically, DNA methylation) changes and their impact on gene expression in testes induced by maternal exposure to Di-2-(ethylhexyl) phthalate (DEHP) mice. Testicular dysgenesis syndrome was fetuses pups DEHP at 500 mg/kg/d, were excised for analysis gestation day (GD) 19 postnatal days (PNDs) 3, 21, 56, 90. High-performance liquid chromatography (HPLC) performed analyze methylation status, levels of the methyltransferases examined quantitative real-time...
Although osteosarcoma (OS) is the most common primary malignancy of bone, its molecular pathogenesis remains to be fully understood. We previously found calcium-binding protein S100A6 was expressed in ∼80% analyzed OS and/or metastatic tumor samples. Here, we investigate role growth and progression.S100A6 expression assessed by qPCR Western blotting. Overexpression or knockdown carried out determine S100A6's effect on proliferation, cell cycle, apoptosis, growth, osteogenic...
Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic progenitor that can undergo self-renewal and differentiate into multi-lineages. Bone marrow stromal (BMSCs) represent one of the most commonly-used MSCs. In order to overcome technical challenge maintaining primary BMSCs in long-term culture, here we seek establish reversibly immortalized mouse (imBMSCs). By exploiting CRISPR/Cas9-based homology-directed-repair (HDR) mechanism, target SV40T Rosa26 locus efficiently immortalize...
Sepsis is a systemic inflammatory response to local severe infection that may lead multiple organ failure and death. Previous studies have shown 40-50% of patients with sepsis diverse myocardial injuries 70 90% mortality rates compared 20% in without injury. Therefore, uncovering the mechanism sepsis-induced injury finding target-based treatment are immensely important. The present study elucidated examined value human umbilical cord mesenchymal stem cells (huMSCs) for protecting cardiac...
Abstract Background Low-intensity pulsed ultrasound (LIPUS) has been reported to accelerate fracture healing, but the mechanism is unclear and its efficacy needs be further optimized. Ultrasound in combination with functionalized microbubbles shown induce local shear forces controllable mechanical stress cells, amplifying effects of LIPUS. Nanoscale lipid bubbles (nanobubbles) have high stability good biosafety. However, effect LIPUS combined nanobubbles on osteogenesis rarely studied....
Multipotent mesenchymal stem cells (MSCs) can undergo self-renewal and give rise to multi-lineages under given differentiation cues. It is frequently desirable achieve a stable high level of transgene expression in MSCs order elucidate possible molecular mechanisms through which MSC lineage commitment are regulated. Retroviral or lentiviral vector-mediated gene usually decreases over time. Here, we choose use the piggyBac transposon system conduct systematic comparison six commonly-used...
The aim of this study was to analyse epigenetic (specifically, DNA methylation) change in testes induced by maternal exposure di-2-(ethylhexyl) phthalate (DEHP) mice. Testicular dysgenesis syndrome foetuses DEHP. High-performance liquid chromatography performed methylation status, and expression levels the methyltransferases were examined quantitative real-time polymerase chain reaction western blotting. DEHP significantly had more than 10% relative increase global also increased...
Abstract Human mesenchymal stem cells (MSCs) are a heterogeneous subset of nonhematopoietic multipotent stromal and can differentiate into mesodermal lineage, such as adipocytes, osteocytes, chondrocytes, well ectodermal endodermal lineages. umbilical cord (UC) is one the most promising sources MSCs. However, molecular cellular characteristics UC‐derived MSCs (UC‐MSCs) require extensive investigations, which hampered by limited lifespan diminished potency over passages. Here, we used...
Abstract The cranial suture complex is a heterogeneous tissue consisting of osteogenic progenitor cells and mesenchymal stem ( MSC s) from bone marrow mesenchyme. fusion sutures highly coordinated tightly regulated process during development. Craniosynostosis congenital malformation caused by premature sutures. While the derived should prove valuable for studying molecular mechanisms underlying development pathogenic fusion, primary human progenitors (SuPs) have limited life span gradually...
Maternally expressed gene 3 (MEG3) is an imprinted with maternal expression, which may function as a tumor suppressor by inhibiting angiogenesis. To identify the prognostic value of MEG3 in breast cancer, systematic analysis was performed this study.To evaluate alteration during carcinogenesis, we explored expression using Serial Analysis Gene Expression Genie suite and Oncomine analysis. The roles cancer were investigated PrognoScan database. heat map methylation status determined UCSC...
Graphene-based materials are used in many fields but have found only limited applications biomedicine, including bone tissue engineering. Here, we demonstrate that novel hybrid consisting of gelatin-derived graphene and silicate nanosheets Laponite (GL) biocompatible promote osteogenic differentiation mesenchymal stem cells (MSCs). Homogeneous cell attachment, long-term proliferation, MSCs on a GL-scaffold were confirmed using optical microscopy scanning electron microscopy. GL-powders made...
Di‐(2‐ethylhexyl) Phthalate Upregulates ATF3 Expression and Suppresses Apoptosis in Mouse Genital Tubercle: Xing L iu , et al . Department of Pediatric Urology, Chongqing Children's Hospital, Medical University, China Objectives To investigate the effect di‐(2‐ethylhexyl) phthalate (DEHP) on expression activating transcription factor 3 (ATF3) apoptosis fetal mouse genital tubercle (GT). Methods In this developmental toxicity study, pregnant C57BL/6 mice were exposed to corn oil or DEHP (100...
It is well known that the immunity of patients with malignant tumors decreases significantly. An increased parvovirus B19 (B19) infection rate has been observed in immunocompromised hosts. However, only a small amount literature regarding risk human available. To evaluate correlation tumors, 288 serum samples from were screened for DNA by nested-PCR. The samples, 156 which clinicopathological cancer patients, subjected to analysis seropositive bocavirus (HBoV), hepatitis B virus (HBV) and...