A5101819666- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Ginseng Biological Effects and Applications
- Gut microbiota and health
- Medicinal Plant Pharmacodynamics Research
- Bone health and osteoporosis research
- Liver Disease Diagnosis and Treatment
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Alzheimer's disease research and treatments
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Heavy Metal Exposure and Toxicity
- Orthopaedic implants and arthroplasty
- Chromatography in Natural Products
- Heavy metals in environment
- Phytochemistry and Biological Activities
- Medical Imaging Techniques and Applications
- Hepatitis Viruses Studies and Epidemiology
- Advanced X-ray and CT Imaging
- Virus-based gene therapy research
- Genetic Associations and Epidemiology
- Phytochemical compounds biological activities
- Cancer-related molecular mechanisms research
- Thyroid Cancer Diagnosis and Treatment
Hubei University of Chinese Medicine
2019-2025
Hubei University of Science and Technology
2023-2025
Fudan University
2020-2025
China Medical University
2025
First Hospital of China Medical University
2025
Second Affiliated Hospital of Nanjing Medical University
2022-2025
Minzu University of China
2025
Capital Medical University
1990-2024
Chang Gung University
2024
Yichun University
2024
Background/Aims: Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into several lineages including bone. Successful bone formation requires osteogenesis and angiogenesis coupling of MSCs. Here, we investigate if simultaneous activation BMP9 Notch signaling yields effective osteogenesis-angiogenesis in Methods: Recently-characterized immortalized mouse adipose-derived (iMADs) were used as MSC source. Transgenes BMP9, NICD dnNotch1 expressed by adenoviral...
With rapid advances in understanding molecular pathogenesis of human diseases the era genome sciences and systems biology, it is anticipated that increasing numbers therapeutic genes or targets will become available for targeted therapies. Despite numerous setbacks, efficacious gene and/or cell-based therapies still hold great promise to revolutionize clinical management diseases. It wildly recognized poor delivery limiting factor most vivo There has been a long-lasting interest using viral...
Hepatotropic viruses naturally have narrow host and tissue tropisms, challenging the development of robust experimental models. The advent organoid technology provides a unique opportunity for moving field forward. Here, we demonstrate that three-dimensional cultured organoids from fetal adult human liver with cholangiocyte or hepatocyte phenotype support hepatitis E virus (HEV) replication. Inoculation infectious HEV particles demonstrates liver–derived full life cycle infection. By...
Sepsis-associated encephalopathy (SAE) is a frequent and nasty complication of sepsis, associated with patients increased risk death long-term brain dysfunctions.This study aimed to explore the effect dexmedetomidine (Dex), an anesthetic adjuvant, on development SAE.Lipopolysaccharide (LPS, 10 mg/kg) was intraperitoneally injected male BALB/c mice induce sepsis. Dex (25 μg/kg) given immediately after LPS injection. Levels TNF-α, IL-1β, malondialdehyde (MDA) reactive oxygen species (ROS) were...
Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic progenitor that can undergo self-renewal and differentiate into multi-lineages. Bone marrow stromal (BMSCs) represent one of the most commonly-used MSCs. In order to overcome technical challenge maintaining primary BMSCs in long-term culture, here we seek establish reversibly immortalized mouse (imBMSCs). By exploiting CRISPR/Cas9-based homology-directed-repair (HDR) mechanism, target SV40T Rosa26 locus efficiently immortalize...
The intestinal microbiome is essential in humans to maintain physiological balance and nutrition metabolism. Laparoscopic cholecystectomy due gallstone disease cholecystitis can cause microbial dysbiosis, following bile acid metabolism dysfunction, positions the patient at high risk of colorectal cancer. However, little known regarding microbiota characteristics post-cholecystectomy patients. Here, we compared composition patients with that a healthy population. We determined eliminated...
The division of the territorial space functional area is primary method to study rational exploitation and use land space. research on Production–Living–Ecological Space (PLES) change its motivating factors has major implications for managing optimizing spatial planning may open up a new direction inquiries into environmental global scale. In this study, transfer matrix landscape pattern index methods were used analyze temporal changes as well evolution features PLES in Chaohu Lake Basin...
Wounds not only harm the physical and mental health of patients, but also introduce huge medical costs. Meanwhile, there is a shortage physicians in some areas, clinical examinations are sometimes unreliable wound diagnosis. Reliable analysis great importance its diagnosis, treatment, care. Currently, deep learning has developed rapidly field computer vision imaging become most commonly used technique image analysis. This paper studies current research on analysis, including classification,...
Abstract Background The hadal sediment, found at an ocean depth of more than 6000 m, is geographically isolated and under extremely high hydrostatic pressure, resulting in a unique ecosystem. Thaumarchaeota are ubiquitous marine microorganisms predominantly present environments. While there have been several studies on there, most them primarily focused ammonia-oxidizing archaea (AOA). However, systematic metagenomic research specifically targeting heterotrophic non-AOA lacking. Results In...
While recombinant adenoviruses are among the most widely-used gene delivery vectors and usually propagated in HEK-293 cells, generating remains time-consuming labor-intense. We sought to develop a rapid adenovirus production amplification (RAPA) line by assessing human Ad5 genes (E1A, E1B19K/55K, pTP, DBP, DNA Pol) OCT1 for their contributions production.Stable transgene expression 293T cells was accomplished using piggyBac system. Transgene determined qPCR. Adenoviral assessed with...
Bioactive casein peptides have attracted considerable attention for their applications in industry. However, there is little clarity regarding mass spectrometric profiles enzymatic hydrolysates of produced under varying conditions. In this study, the compositions from were compared different enzyme/substrate ratio (E/S) and hydrolysis times. The toxicity, allergenicity bioactivity identified assessed silico.A total 70 unique identified, 28, 21, 13 8 αs1 -casein, αs2 β-casein κ-casein...
Mesenchymal progenitor stem cells (MPCs) are a group of bone marrow stromal processing osteogenic, chondrogenic, adipogenic and myogenic lineages differentiations.Previous studies have demonstrated that morphogeneic protein 9(BMP9) is one the most osteogenic BMPs both in vitro vivo, however, underlying molecular mechanism osteogenesis induced by BMP9 needed to be deep explored.Here, we used recombinant adenoviruses assay introduce into C3H10T1/2 mesenchymal elucidate role CXCL12/CXCR4 signal...
Bone mineral density (BMD) alterations in response to multivitamin exposure were rarely studied. Our study assessed the association of coexposure six types vitamins (i.e., B12, B9, C, D, A and E) with BMD measurements adults US.Data collected from participants aged ≥ 20 years (n = 2757) U.S. National Health Nutrition Examination Surveys (NHANES) 2005 2006. Multiple linear regression, restricted cubic splines, principal component analysis (PCA) weighted quantile sum (WQS) regression performed...
Coumarin skeletons are significant structural units frequently used in the fields of synthetic chemistry, medicinal and materials science. Herein, we report a hemilabile P,N-ligand-assisted gold-catalyzed difunctionalization activated alkynes with organohalides. The reaction occurs effectively under mild conditions without requiring an external oxidant, producing variety 3-arylated alkenylated coumarin derivatives high to excellent yields. This method demonstrates wide substrate tolerance...