A5108103201- Muscle Physiology and Disorders
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Genetic and Kidney Cyst Diseases
- Hedgehog Signaling Pathway Studies
- Tissue Engineering and Regenerative Medicine
- Ubiquitin and proteasome pathways
- Nutrition, Genetics, and Disease
- Nutrition and Health in Aging
- Exercise and Physiological Responses
- Renal and related cancers
- High Altitude and Hypoxia
- Cardiomyopathy and Myosin Studies
- Genetic Syndromes and Imprinting
- Vitamin D Research Studies
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Adipose Tissue and Metabolism
- Pluripotent Stem Cells Research
- Heavy Metal Exposure and Toxicity
- Epigenetics and DNA Methylation
- Transgenic Plants and Applications
- Biomedical Research and Pathophysiology
Nanjing University
2021-2024
Soochow University
2008-2024
First Affiliated Hospital of Soochow University
2008-2024
Southwest University
2020-2023
Chinese Academy of Sciences
2012-2023
Chongqing Institute of Green and Intelligent Technology
2023
Central South University
2022-2023
Second Xiangya Hospital of Central South University
2023
Soil and Fertilizer Institute of Hunan Province
2023
Sichuan Agricultural University
2010-2022
Mutations inPKD2cause autosomal dominant kidney disease (ADPKD). Polycystin-2, thePKD2gene product, is an integral membrane glycoprotein of unknown function. We have identifiedPKD2L, another member family.PKD2Lis expressed in adult heart and skeletal muscle, brain, spleen, testis, retina, alternative transcripts 2.4, 2.7, 3.0 kb are seen. PKD2L shows 56% identity 76% similarity with polycystin-2 over a 581-amino-acid span; however, the COOH-terminal 65 residues unrelated to PKD2.PKD2Lis...
Differentiation of skeletal muscle cells is inhibited by the cyclic AMP (cAMP) signal transduction pathway. Here we report that catalytic subunit cAMP-dependent protein kinase (PKA) can substitute for cAMP and suppress muscle-specific transcription silencing activity MyoD family regulatory factors, which includes MyoD, myogenin, myf5, MRF4. Repression PKA (C) directed at consensus sequence CANNTG, target DNA binding transcriptional activation these myogenic regulators. Phosphopeptide mapping...
While recombinant adenoviruses are among the most widely-used gene delivery vectors and usually propagated in HEK-293 cells, generating remains time-consuming labor-intense. We sought to develop a rapid adenovirus production amplification (RAPA) line by assessing human Ad5 genes (E1A, E1B19K/55K, pTP, DBP, DNA Pol) OCT1 for their contributions production.Stable transgene expression 293T cells was accomplished using piggyBac system. Transgene determined qPCR. Adenoviral assessed with...
PKD2 encodes a protein of unknown function that is mutated in 15% autosomal dominant polycystic kidney disease (ADPKD) families. We used polyclonal antisera against to examine the pattern Pkd2 expression staged mouse embryos. Staining for was documented as early 6th embryonic day (day E6) ectoderm and endoderm. Low-intensity staining seen metanephric ureteric bud at E12.5. By E15.5, adult established with low level proximal tubules high level, basolateral distal tubules. first detected...
Abstract Previous studies have shown that lipid metabolism in granulosa cells (GCs) plays a vital role during mammalian ovarian follicular development. However, little research has been done on avian GCs. The goal of the present study was to investigate dynamic characteristics GCs from geese pre-hierarchical (6–10 mm) and hierarchical (F4-F2 F1) follicles 6-day period vitro culture. Oil red O staining showed with increasing incubation time, amount lipids accumulated three cohorts increased...
Abstract Phenotypic screening of cell-cell and cell-matrix interactions is critical yet challenging for drug discovery disease modeling. In this study, a scalable 3D tissue platform was developed by co-laying extracted natural insoluble collagen fibers, mesenchymal stem cells, endothelial neural progenitor cells phenotypic screening. Cell growth were enhanced in the co-laid platform, evident through increased cell proliferation, viability, vascularization. Dense vascular networks rapidly...
Background and Purpose Cancer cachexia is a common cause of death among cancer patients with no currently effective treatment available. In animal models, aberrant activation STAT3 in skeletal muscle contributes to wasting. However, clinically the factors regulating molecular mechanisms involved remain incompletely understood. Experimental Approach The expression HSP90 were detected from or tumour‐bearing cachectic mice. inhibitors, including 17DMAG (alvespimycin) PU‐H71, administered...
Abstract Cancer cachexia is a multifactorial metabolic syndrome that causes up to 20% of cancer-related deaths. Muscle atrophy, the hallmark cancer cachexia, strongly impairs quality life patients; however, underlying pathological process still poorly understood. Investigation disease pathogenesis largely relies on cachectic mouse models. In our study, transcriptome gastrocnemius muscle in C26 xenograft model was integrated and compared with 5 more different datasets. The bioinformatic...
Sugar, the most popular sweetener, is essential in daily food. However, excessive sugar intake has been associated with several lifestyle-related diseases. Finding healthier and more economical alternatives to sugars artificial sweeteners received increasing attention fulfill growing demand. Brazzein, which comes from pulp of edible fruit African plant Pentadiplandra brazzeana Baill, a protein that 2,000 times sweeter than sucrose by weight. Here we report production transgenic mice carry...
Cancer cachexia is a lethal metabolic syndrome featuring muscle wasting with preferential loss of fast-twitching mass through an undefined mechanism. Here, we show that cancer induces by selectively degrading myosin heavy chain (MHC) subtypes IIb and IIx E3 ligase UBR2-mediated ubiquitylation. Induction MHC atrophy in C2C12 myotubes mouse tibialis anterior (TA) murine cells required UBR2 up-regulation cancer. Genetic gain or function inversely altered level TA tumor-free mice. interacted...
Sharp-1 is a basic helix-loop-helix (bHLH) transcriptional repressor which involved in number of cellular processes. Our previous studies have demonstrated that negative regulator skeletal myogenesis and blocks differentiation muscle precursor cells by modulating MyoD activity. In order to understand its role pre- post-natal myogenesis, we assessed development, freeze-injury induced regeneration deficient mice. We show embryonic development not impaired the absence Sharp-1, but post-natally,...