Avian species exhibit a notable asymmetry in their reproductive systems, with the left ovary maturing into functional organ and right degenerating. This is widespread across bird species, yet molecular underpinnings of ovarian degeneration are not fully understood. In this study, we constructed cellular atlas degenerating geese through single-cell transcriptome sequencing (scRNA-seq), revealing distinct compositions gene expression profiles. Meanwhile, identified variations rate among...

Age at first egg (AFE) has consistently garnered interest as a crucial reproductive indicator within poultry production. Previous studies have elucidated the involvement of hypothalamic-pituitary-ovarian (HPO) and hypothalamic-pituitary-thyroid (HPT) axes in regulating sexual maturity. Concurrently, there was evidence suggesting potential co-regulatory relationship between these 2 axes. However, now, no comprehensive exploration key pathways genes responsible for crosstalk HPO HPT regulation...

miRNAs are critical for steroidogenesis in granulosa cells (GCs) during ovarian follicular development. We have previously shown that miR-202-5p displays a stage-dependent expression pattern GCs from goose follicles of different sizes, suggesting this miRNA could be involved the regulation functions GCs; therefore, study, effects on lipid metabolism and hierarchical (hGCs), as well its mechanisms action, were evaluated. Oil Red O staining analyses intracellular cholesterol triglyceride...

Follicle development is characterized by the recruitment, growth, selection, and dominance of follicles, follicle selection determines lifetime reproductive performance. However, in birds, molecular mechanisms underlying still remain elusive. This study analyzed genome-wide changes mRNA miRNA expression profiles both granulosa theca layers geese ovarian follicles before (4-6- 8-10-mm follicles) after (F5). The sequencing results showed that a higher number differentially expressed (DE) mRNAs...

To date, research on poultry egg production performance has only been conducted within inter or intra-breed groups, while those combining both inter- and groups are lacking. Egg is known to differ markedly between Sichuan white goose (Anser cygnoides) Landes anser). In order understand the mechanism of in geese, we undertook this study. Here, 18 ovarian stromal samples from at age 145 days (3 individuals before initiation for each breed) 730 high- low during non-laying periods were collected...

Abstract Previous studies have shown that lipid metabolism in granulosa cells (GCs) plays a vital role during mammalian ovarian follicular development. However, little research has been done on avian GCs. The goal of the present study was to investigate dynamic characteristics GCs from geese pre-hierarchical (6–10 mm) and hierarchical (F4-F2 F1) follicles 6-day period vitro culture. Oil red O staining showed with increasing incubation time, amount lipids accumulated three cohorts increased...

Abstract Background All birds reproduce via internal fertilization, but only ~3% of male possess the external genitalia that allows for intromission. Waterfowl (e.g., duck and goose) are representatives them, development geese is directly related to mating ability. Notably, some show abnormal during ontogenesis. However, until now little known about molecular mechanisms in goose. In present study, comparative transcriptomic analyses were performed on hypothalamus, pituitary gland, testis,...

Eggs are essential food sources as they provide low cost and high nutritional content of animal protein. The preservation period is one the apparent factors affecting egg quality. Previous studies based on traditional detection techniques demonstrated that storage would significantly influence weight, eggshell albumen height, haugh unit (HU) viscosity. Herein, we employed non-targeted metabolome technology to reveal comprehensive changes in metabolite composition duck eggs under impacts...

PLIN1, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate regulatory mechanism PLIN1 goose follicular GCs. Gene Ontology (GO) analysis revealed that four groups (phGC: over_vs_over-NC; hGC: phGC: si_vs_si-NC; si_vs_si-NC), most differentially genes (DEGs) were significantly enriched (p < 0.05) pathways related biological processes (BPs), particularly...

Semen quality has an important impact on the reproductive performance of ganders, and quantity spermatozoa in semen are determinants quality. In our practical work, a small number azoospermic ganders were observed adult goose breeding populations, but underlying regulatory mechanisms remain unknown. present study, we firstly compared morphological histological differences testes from normozoospermic group (NG) (AG), then analyzed testicular expression patterns circRNAs, miRNAs, mRNAs between...

Sperm motility is a critical indicator of semen quality and determines the reproduction performance in male poultry. However, compared to chickens ducks, very little known about physiological basis varying levels sperm as well underlying regulatory mechanisms geese. To address this, present study, systematic comparison parameters histomorphological characteristics genome-wide transcriptomic profiles testes epididymis were performed ganders with high low motility. Our results showed that...

Avian hierarchical follicles are formed by selection and dominance of pre-hierarchical follicles, lipid metabolism plays a pivotal role in this process. The amount goose follicular granulosa cells increases with the increase culture time, neutral is stored form droplets (LDs). LD-associated proteins (LDAPs) collaborate LDs to regulate intracellular homeostasis, which subsequently influences avian follicle development. mechanism LDAPs at different developmental stages unclear. Therefore,...

Meat color is the most intuitive measure of meat quality and has a significant impact on consumer preference. To explore molecular mechanisms affecting duck pectoralis color, phenotypic traits Cherry Valley (CV, eight males females) Huai Fu (HF, were compared; three females each variety later selected for transcriptomic metabolomic analyses to reveal key processes. This study found that expression level CA3 (carbonic anhydride enzyme 3) positively correlated with phenotype, suggesting it may...

De novo lipogenesis (DNL) is an important physiological mechanism, but it poorly understood in avian follicles. The protein distribution patterns of three key genes related to DNL (i.e., FAS, ACC, and PPARγ) were firstly determined geese developing follicles using immunohistochemistry, our results showed that all proteins present both prehierarchical hierarchical Furthermore, was revealed by qPCR transcripts these widely expressed theca granulosa layers staged follicles; however, the...

In contrast to the situation in mammals, very little is known about molecular mechanisms regulating early avian ovarian development. This study aimed investigate dynamic changes histomorphology as well genome-wide transcriptome and chromatin accessibility landscapes of goose ovary during late embryonic post-hatching stages. Results from hematoxylin-eosin, periodic acid-Schiff, anti-CVH immunohistochemical stainings demonstrated that programmed oocyte loss, nest breakdown primordial follicle...

Due to the demand for modern goose production and pressure of environmental protection, rearing systems geese are changing from traditional waterside intensive such as floor (FR) cage (CR) systems. However, little is known about effects different on intestinal functions cecal microbial composition. Therefore, this study aimed compare histomorphology composition differences in reared under CR FR at 270 d age. Histomorphological analysis showed that ileal villus height (VH) crypt depth (CD)...

As the largest peripheral lymphoid organ in poultry, spleen plays an essential role regulating body’s immune capacity. However, compared with chickens and ducks, information about age- breed-related changes goose remains scarce. In this study, we systematically analyzed age-dependent morphological, histological, transcriptomic characteristics between Landes (LG; Anser anser ) Sichuan White (SWG; cygnoides ). The results showed a gradual increase splenic weights for both LG SWG until week 10,...

Early research in our lab indicated that the effect of glucose, fructose and sucrose on levels triacylglycerol, inflammatory factor was significantly different, it is speculated regulatory mechanism lipid deposition by different type sugar liver different. In order to explore difference mediated types (glucose, fructose, sucrose) goose fatty formation, this experiment performed from cell culture, overfeeding experiment, transcriptome analysis at 3 levels. Cell culture results intracellular...

Abstract Background Egg production is one of the most important economic traits in poultry industry. The hypothalamic-pituitary–gonadal (HPG) axis plays an essential role regulating reproductive activities. However, key genes and regulatory pathways within HPG dominating egg performance remain largely unknown ducks. Results In this study, we compared transcriptomic profiles HPG-related tissues between ducks with high (HEP) low (LEP) to reveal candidate production. We identified 543, 759,...

Evidences have found important effects of breeds/strains on the content amino acids (AAs) which is an substrate for protein synthesis and contributes greatly to meat quality. Therefore, objective present study was compare AAs synthesis-related genes expression levels in breast muscle native breed (Jianchang duck (J)), hybrid strains (BH1, BH2, MC♂ × (BGF2♂ GF2♀)♀ (MC)), commercial (Cherry Verry duck). Results showed that a total 17 (TAAs) detected from among 5 including 11 essential (EAAs)....

The regulation of granulosa cells (GCs) proliferation and apoptosis is the key step in follicular selection which determines egg production performance poultry. miR-202-5p has been reported to be involved regulating mammalian ovarian GCs. However, its role goose GCs still unknown. In present study, pre-hierarchical follicles (phGCs, 8-10 mm) those hierarchical (hGCs, F2-F4) were used investigate cell during follicle selection. phGCs hGCs cultured vitro, was found negatively regulate...

Granulosa cells (GCs) are essential components of follicles and involved in regulating the process development. However, comparative studies on GCs isolated from different staged have not been conducted goose. The aim present study was to identify growth characteristics goose pre-hierarchical (6–10 mm) hierarchical (F4–F2, F1) follicles. Our results showed that three cohorts had tolerance collagenase noticeable morphological differences. F1 granulosa layers were fully digested by 0.1%...