PLIN1, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate regulatory mechanism PLIN1 goose follicular GCs. Gene Ontology (GO) analysis revealed that four groups (phGC: over_vs_over-NC; hGC: phGC: si_vs_si-NC; si_vs_si-NC), most differentially genes (DEGs) were significantly enriched (p < 0.05) pathways related biological processes (BPs), particularly...

Avian hierarchical follicles are formed by selection and dominance of pre-hierarchical follicles, lipid metabolism plays a pivotal role in this process. The amount goose follicular granulosa cells increases with the increase culture time, neutral is stored form droplets (LDs). LD-associated proteins (LDAPs) collaborate LDs to regulate intracellular homeostasis, which subsequently influences avian follicle development. mechanism LDAPs at different developmental stages unclear. Therefore,...

Spontaneous preterm labor (PTL) accounts for approximately 70% of births, posing significant risks to both maternal and neonatal health. Current predictive biomarkers lack sufficient reliability, underscoring the need non-invasive dependable indicators. Emerging research indicates that tRNA-derived small RNAs (tsRNAs) are involved in various diseases; however, their potential association with PTL remains underexplored. Bioinformatics analyses public GEO datasets (PRJNA415953 PRJNA428989)...

The regulation of granulosa cells (GCs) proliferation and apoptosis is the key step in follicular selection which determines egg production performance poultry. miR-202-5p has been reported to be involved regulating mammalian ovarian GCs. However, its role goose GCs still unknown. In present study, pre-hierarchical follicles (phGCs, 8-10 mm) those hierarchical (hGCs, F2-F4) were used investigate cell during follicle selection. phGCs hGCs cultured vitro, was found negatively regulate...

Although miR-27b-3p has been evidenced to regulate the proliferation, apoptosis, and differentiation of a variety mammalian cell types, its actions mechanisms on ovarian steroidogenesis remains largely unknown in both avian species. In this study, we aimed determine expression profiles granulosa layers during goose follicle development reveal estrogen (E2) secretion cells as well underlying regulatory mechanisms. It was observed that ubiquitously expressed throughout but exhibited much...

Due to the demands for both environmental protection and modernization of goose industry in China, traditional waterside rearing systems have been gradually transitioning modern intensive dryland ones, such as net-floor mixed system (MRS) cage (CRS). However, immune responses different remain poorly understood. This study aimed investigate compare age-dependent effects MRS CRS on splenic histomorphological characteristics immune-related genes expression profiles among three economically...

Recurrent implantation failure (RIF) refers to conceive after three or more in vitro fertilisation (IVF) embryo transfer cycles. Implantation may be due uterine factors. There are many controversies surrounding the investigation and management of this condition. The aim our study was describe clinical characteristics outcome investigations a group women with recurrent failure. A total 111 couples RIF were managed dedicated clinic investigated according protocol. frequency abnormal as...

Abstract Background Despite their important functions and nearly ubiquitous presence in cells, an understanding of the biology intracellular lipid droplets (LDs) goose follicle development remains limited. An integrated study lipidomic transcriptomic analyses was performed a cellular model stearoyl-CoA desaturase (SCD) function, to determine effects LDs on geese. Results Numerous internalized LDs, which were generally spherical shape, dispersed throughout cytoplasm granulosa cells (GCs), as...

The development of the follicular wall and apoptosis corresponding cells are dependent upon stage follicle growth levels endogenous hormones. However, prehierarchical follicles in geese is insufficiently known. In order to obtain an understanding about microstructure, geese, firstly, a histological method was used investigate morphological structure follicles. Results showed that thickness granulosa cell layers increased first, then decreased lowest when grew 9-10 mm diameter, theca also...

Stearoyl-CoA desaturase (SCD) is known to be an important rate-limiting enzyme in the production of monounsaturated fatty acids (MUFAs). However, role this goose follicular development poorly understood. To investigate metabolic mechanism SCD during development, we observed its expression patterns vivo and vitro using quantitative reverse-transcription (qRT)-PCR. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used determine a cellular model function granulosa cells (GCs) via...

Stearoyl-CoA desaturase (SCD) is a key enzyme catalyzing the rate-limiting step in monounsaturated fatty acids (MUFAs) production. There may be mechanism by which SCD involved lipid metabolism, assumed to essential for goose follicular development. For this reason, cellular model of function granulosa cells (GCs) via overexpression and knockdown was used determine role GC proliferation using flow cytometry. We found that induced inhibited GCs proliferation. Furthermore, ELISA analysis showed...

The transcription factors GATA-4 and GATA-6, members of the GATA family, play an important role in ovarian cell proliferation, differentiation apoptosis. In this study, full-length coding sequences goose GATA-6 were cloned characterized. consist 1236 1104 nucleotides encoding proteins with 411 367 amino acids, respectively. deduced acid both include two adjacent zinc finger domains distinctive form (CVNC-X17-CNAC)-X29-(CANC-X17-CNAC) share 84.76% identity within domain. silico prediction...

This study explores the molecular interplay between systemic lupus erythematosus (SLE) and osteoporosis (OP), aiming to uncover shared gene signatures pathways for better treatment approaches. Leveraging microarray data from Gene Expression Omnibus (GEO) database, we employed weighted coexpression network analysis identify modules in SLE OP, with subsequent protein-protein interaction clarifying connections among genes. Key genes were pinpointed using CytoHubba random forest algorithms,...

Recurrent miscarriage is often unexplained. In our study, we test the hypothesis that unexplained recurrent associated with premature ovarian ageing by comparing basal follicle-stimulating hormone (FSH) level between women and a control group of subjects in two different ethnic centres: one Guangzhou, China other Sheffield, UK. The were stratified into three age groups, all them under 40 years. We found no significant difference FSH proportion high (> 10 IU/l) was higher Guangzhou cohort but...

In poultry, prolactin (PRL) plays a key role in the regulation of incubation behavior, hormone secretion, and reproductive activities. However, previous vitro studies have focused on actions PRL ovarian follicles relying use exogenous or recombinant PRL, true regulating granulosa cell (GC) functions poultry awaits further investigation using endogenous native PRL. Therefore, this study, we first isolated purified goose protein (rPRL) (nPRL) Ni-affinity chromatography rabbit anti-rPRL...

Abstract Background Stearoyl-CoA desaturase (SCD) is known to be an important rate-limiting enzyme in the production of MUFA. The role this goose follicular development poorly understood. To investigate metabolic mechanism SCD during development, we observed expression patterns vivo and vitro using quantitative reverse-transcription (qRT)-PCR. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used determine a cellular model function granulosa cells (GCs) via overexpression...

Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence Peking duck FAS and investigate its expression during adipocyte differentiation. We have isolated 7654 bp fragment adipocytes that corresponds gene. contains an open reading frame 7545 bp, encodes 2515 amino protein, displays high...

1. Although PRL-PRLR signalling plays important roles in regulating avian reproduction, there is a paucity of information regarding the functional significance PRLR goose ovarian follicle development.2. The full-length 2,496 bp coding sequence was obtained from Sichuan White (Anser cygnoides) for first time and seen to encode polypeptide containing 831 amino acids. Goose shares similar characteristics conserved domains other species phylogenetically clustered into clade.3. qPCR results...

1. The present study was conducted to obtain a better understanding of the molecular mechanisms underlying broodiness in commercial breed, Tianfu geese, as little is known about role pineal gland this period. aim identify genes which are differentially expressed between laying and periods by performing transcriptome screen.2. After sequencing cDNA derived from annotation results, depth 14.82 18.17 million mapped tags obtained during periods, respectively, total 120 were identified. Of these,...

Abstract Despite their important functions and nearly ubiquitous presence in cells, an understanding of the biology intracellular lipid droplets (LDs) goose follicle development remains limited. An integrated study lipidomic transcriptomic analyses was performed a cellular model stearoyl-CoA desaturase (SCD) function, to determine effects LDs on geese. Numerous internalized LDs, which were generally spherical shape, dispersed throughout cytoplasm granulosa cells (GCs) by confocal microscopy...