A5100379364- Reproductive Biology and Fertility
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Sperm and Testicular Function
- Reproductive System and Pregnancy
- Acupuncture Treatment Research Studies
- Prenatal Screening and Diagnostics
- Phagocytosis and Immune Regulation
- Heat shock proteins research
- RNA Research and Splicing
- Cancer Research and Treatments
- HIV Research and Treatment
- Cancer Mechanisms and Therapy
- Traditional Chinese Medicine Analysis
- Birth, Development, and Health
- Cancer-related molecular mechanisms research
- Healthcare and Venom Research
- RNA modifications and cancer
- Traditional Chinese Medicine Studies
- MicroRNA in disease regulation
- Diet, Metabolism, and Disease
- Immune Cell Function and Interaction
- Aquatic Ecosystems and Phytoplankton Dynamics
- Immunotherapy and Immune Responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Southern Medical University
2020-2025
Nanjing General Hospital of Nanjing Military Command
2016-2025
Nanjing Medical University
2016-2025
Nanjing University
2015-2025
Guangdong Provincial Hospital of Traditional Chinese Medicine
2022-2025
Fujian Medical University
2022-2025
Xiamen University of Technology
2023-2025
Huazhong University of Science and Technology
2025
Nanchang University
2015-2025
First Affiliated Hospital of Nanchang University
2025
Significance In in vitro fertilization (IVF), current methods of diagnosing chromosome abnormality and screening for viability transfer require biopsy embryos, which affects embryo quality, awaits long-term biosafety test, requires specialized skills. We demonstrate the principle noninvasive (NICS), is based on sequencing genomic DNA secreted into culture medium from embryo, avoiding need substantially increasing safety. By characterizing its precision demonstrating successful live births,...
As the chemical environment of semen can have a profound effect on sperm quality, we examined pH motility, viability and capacitation human sperm. The in this study was collected from healthy males to avoid interference other factors. spermatozoa cultured nutrition solution at 5.2, 6.2, 7.2 8.2 were analyzed for total progressive motility (PR), hypo-osmotic swelling (HOS) rate, penetration. Our results showed that these parameters similar solutions, but decreased 5.2 6.2 solutions. HOS rate...
Oxidative stress is known as a major contributing factor involved in oocyte aging, which negatively affects quality and development after fertilization. Melatonin an effective free radical scavenger its metabolites AFMK AMK are powerful detoxifiers that eliminate radicals. In this study, we used porcine oocytes to test the hypothesis melatonin could scavenge radicals produced during thereby maintaining quality. We compared reactive oxygen species levels, apoptosis mitochondrial membrane...
To successfully prepare the diclofenac sodium (DS)-loaded solid lipid nanoparticles (SLNs), phospholipid complexes (PCs) technology was applied here to improve liposolubility of DS. Solid (SLNs) loaded with were prepared by modified emulsion/solvent evaporation method. DS could be solubilized effectively in organic solvents existence and apparent partition coefficient PCs increased significantly. X-ray diffraction analysis suggested that either molecularly dispersed or an amorphous form....
Many factors may lead to sperm DNA damage. However, it is little known that the correlations of damage with obesity-associated markers, and reproductive hormones lipids levels in serum seminal plasma. In our prospective study, a total 1 010 subfertile men, aged from 18 50 years old, were enrolled August 2012 through June 2015. Their semen parameters, acrosomal enzyme activity, plasma biochemical detected. Sperm fragmentation index (DFI) was determined by chromatin structure assay. The...
Vitamin D deficiency is a common health issue around the world. We therefore evaluated associations of semen quality with both serum and seminal plasma vitamin levels studied mechanisms underlying these by incubating spermatozoa 1,25(OH)2D in vitro. Two hundred twenty-two men were included our study. was detected using an electrochemiluminescence method. Spermatozoa used for vitro experiments isolated density gradient centrifugation. Positive relationships 25(OH)D volume fructose identified....
To investigate the effects of maternal age on quality oocytes, we used single-cell RNA sequencing to detect global gene transcriptome and identify key genes affected by advanced in human mature oocytes.We isolated mRNA from oocytes obtained IVF or ICSI patients (three younger (≤30 years) three older (≥40 for scRNA-seq.We identified 357 differentially expressed between matured women's.The up-regulated were significantly enriched with annotations related transcriptional activation, oxidative...
Previous studies from this as well other research groups suggested that non-invasive chromosome screening (NICS) with embryo culture medium can be used to identify chromosomal ploidy and abnormalities. We here report a series of clinical cases utilizing the technology.A total 45 couples underwent in vitro fertilisation during period between February 2016 2017. Karyotyping revealed normal chromosomes both partners 23 couples, rearrangements at least one partner 22 couples. Intracytoplasmic...
Blood-testis barrier (BTB) damage promotes spermatogenesis dysfunction, which is a critical cause of male infertility. Dyslipidemia has been correlated with infertility, but the major hazardous lipid and underlying mechanism remains unclear. In this study, we firstly discovered an elevation palmitic acid (PA) decrease inhibin B in patients severe dyszoospermia, leaded us to explore effects PA on Sertoli cells. We observed BTB by PA. penetration endoplasmic reticulum (ER) its ER structures...
Abstract Glycosylation is currently considered to be an important hallmark of cancer. However, the characterization glycosylation-related gene sets has not been comprehensively analyzed in glioma, and relationship between genes glioma prognosis elucidated. Here, we firstly found that differentially expressed patients were engaged biological functions related progression revealed by enrichment analysis. Then seven glycosylation ( BGN , C1GALT1C1L GALNT13 SDC1 SERPINA1 SPTBN5 TUBA1C )...
There is increasing evidence that mesenchymal stem cells (MSCs) derived from different tissues could act as an alternative source of mature hepatocytes for treatment acute liver failure (ALF). Human umbilical cord matrix (hUCMSCs) represent a novel MSCs. We examined the therapeutic potential and mechanisms hUCMSCs by their transplantation into nonobese diabetic severe combined-immunodeficient (NOD-SCID) mice with carbon tetrachloride (CCl(4))-induced ALF in comparison to adult human (AHHs)....
Abstract DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5) is an ATP-dependent RNA that overexpressed in various malignancies. Increasing evidence suggests DDX5 participates carcinogenesis and cancer progression via promoting cell proliferation metastasis. However, the functional role of gastric largely unknown. In this study, we observed was significantly up-regulated tissues compared with paired adjacent normal tissues. The expression correlated strongly Ki67 index pathological stage cancer....
Neutrophil extracellular traps (NETs) play an important role in the pathological process of atherosclerosis (AS). This study aims to evaluate whether exosomes from oxidized low-density lipoprotein (ox-LDL)-treated vascular endothelial cells (VECs) aggravate AS by inducing NET formation. Exosomes peripheral blood healthy donors and patients (namely NC-EXO AS-EXO, respectively) human umbilical vein (HUVECs) treated without or with ox-LDL normal EXO ox-LDL-EXO, were isolated, identified,...
Ginkgo biloba L., well known as living fossil, have various pharmacological activities. Eighteen compounds were isolated from male flowers including a novel matsutake alcohol glycoside, Ginkgoside A ( 1 ), and 17 compounds—calaliukiuenoside 2 benzylalcohol O ‐α‐ l ‐arabinopyranosyl‐(1 → 6)‐β‐ d ‐glucopyranoside 3 amentoflavone 4 sciadopitysin 5 bilobetin 6 isoginkgetin 7 olivil 4‐ ‐β‐ 8 dihydrodehydrodiconiferyl alcohol‐4‐ ‐glucoside 9 (+)‐cyclo‐olivil‐6‐ 10 (−)‐isolariciresinol 11 coniferin...
Objective This prospective study was designed to investigate the relationship between lipids levels in both serum and seminal plasma semen parameters. Methods 631 subfertile men were enrolled. Their obesity-associated markers measured, parameters analyzed. Also, TC, TG, HDL LDL FFA, FSH, LH, total testosterone (TT), estradiol (E2) SHBG detected. Results Seminal TC positively related age. Serum TG (P < 0.001), while only them 0.05). For plasma, level had slightly positive correlation (r =...
Background: Triple-negative breast cancer (TNBC) is an aggressive malignancy with high heterogeneity. However, the alternative polyadenylation (APA) profiles of TNBC remain unknown. Here, we aimed to define characteristics APA events at post-transcription level among TNBCs. Methods: Using transcriptome microarray data, analyzed 165 samples and 33 paired normal tissues. A pooled short hairpin RNA screen targeting 23 core cleavage (C/P) genes was used identify key C/P factors. Results: We...
Abstract It has been widely acknowledged that anti-Müllerian hormone (AMH) is a golden marker of ovarian reserve. Declined reserve (DOR), based on experience from reproductive-aged women, refers to both the quantitative and qualitative reduction in oocytes. This view challenged by recent study clearly showing quality oocytes similar young women undergoing IVF cycles irrespective level AMH. However, it remains elusive whether AMH indicates oocyte with advanced age (WAA). The aim this was...
Heat shock transcription factors (HSFs) play important roles in plant growth, development, and stress responses. However, the function of these abiotic responses maize (Zea mays) remains largely unknown. In this study, we characterized a novel HSF factor gene, ZmHsf08, from maize. ZmHsf08 was highly homologous to SbHsfB1, BdHsfB1, OsHsfB1, has no transcriptional activation activity. The expression profiles demonstrated that differentially expressed various organs induced by salt, drought,...