A5103032171- Prenatal Screening and Diagnostics
- Genomic variations and chromosomal abnormalities
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Genetic diversity and population structure
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Crustacean biology and ecology
- RNA and protein synthesis mechanisms
- Immune cells in cancer
- Advanced biosensing and bioanalysis techniques
- Cancer Immunotherapy and Biomarkers
- Fungal and yeast genetics research
- Mesenchymal stem cell research
- Plant and Fungal Species Descriptions
- Liver Disease Diagnosis and Treatment
- Cancer-related gene regulation
- Circular RNAs in diseases
- Pharmacological Receptor Mechanisms and Effects
- Gene Regulatory Network Analysis
- Sesquiterpenes and Asteraceae Studies
Shanghai Pulmonary Hospital
2020-2025
Tongji University
2018-2025
China Pharmaceutical University
2025
Peking University
2015-2024
University of Shanghai for Science and Technology
2023-2024
Northwest A&F University
2021-2023
Jinan University
2023
Wuhan University
2023
Sichuan Cancer Hospital
2022
University of Chicago
2022
Making an unbiased library Sequencing the genome of single cells gives insight into issues such as cell-to-cell heterogeneity and instability. Key to single-cell sequencing techniques are whole-genome amplification (WGA) methods that provide sufficient DNA for next-generation sequencing. Current WGA have been hampered by low accuracy spatial resolution gene copy numbers fidelity. Chen et al. report improved method, Linear Amplification via Transposon Insertion (LIANTI). The is randomly...
Significance In in vitro fertilization (IVF), current methods of diagnosing chromosome abnormality and screening for viability transfer require biopsy embryos, which affects embryo quality, awaits long-term biosafety test, requires specialized skills. We demonstrate the principle noninvasive (NICS), is based on sequencing genomic DNA secreted into culture medium from embryo, avoiding need substantially increasing safety. By characterizing its precision demonstrating successful live births,...
Preimplantation genetic testing for aneuploidy (PGT-A) with trophectoderm (TE) biopsy is widely applied in vitro fertilization (IVF) to identify aneuploid embryos. However, potential safety concerns regarding and restrictions only those embryos suitable pose limitations. In addition, embryo mosaicism gives rise false positives negatives PGT-A because the inner cell mass (ICM) cells, which give fetus, are not tested. Here, we report a critical examination of efficacy noninvasive...
Significance One missing or wrong nucleotide out of six billion in a human genome can cause genetic disease. Detecting such point mutation single germ cell has been daunting challenge vitro fertilization, yet one cannot afford to make any mistakes selecting viable embryo for transfer. Mutated allele revealed by sequencing with aneuploidy and linkage analyses (MARSALA) combines next-generation single-cell whole-genome amplification methodologies, allowing diagnosis single-molecule precision,...
In recent years, immune-based therapies have become an increasingly attractive treatment option for patients with cancer. Cancer immunotherapy is often used in combination conventional chemotherapy synergistic effects. The alkylating agent cyclophosphamide (CTX) has been included various chemoimmunotherapy regimens because of its well-known immunostimulatory Paradoxically, can also induce suppressor cells that inhibit immune responses. However, the identity and biologic relevance these are...
Reciprocal translocations (RecT) and Robertsonian (RobT) are among the most common chromosomal abnormalities that cause infertility birth defects. Preimplantation genetic testing for aneuploidy using comprehensive chromosome screening in vitro fertilization enables embryo selection with balanced ploidy; however, it is normally unable to determine whether an a translocation carrier. Here we report method named "Mapping Allele Resolved Carrier Status" (MaReCs), which ploidy resolution of...
Immune checkpoint blockade has shown remarkable efficacy, but in only a minority of patients with cancer, suggesting the need to develop additional treatment strategies. Aberrant glycosylation tumors, resulting from dysregulated expression key enzymes glycan biosynthesis, modulates immune response. However, role biosynthesis antitumor immunity is poorly understood. We aimed study immunomodulatory effects these enzymes.We integrated transcriptional profiles treatment-naïve human tumors and...
Mesenchymal stem cells (MSCs) can migrate to tissue injury sites where they induce multipotential differentiation and anti-inflammation effects treat injury. When traditional therapeutic methods do not work, MSCs are considered be one of the best candidates for cell therapy. have been used treating several injury- inflammation-associated diseases, including liver cirrhosis. However, effect is limited. In some cases, anti-inflammatory function naïve enough rescue Carbon tetrachloride (CCl4)...
Indoleamine 2,3-dioxygenase-1 (IDO1) plays a crucial role in tumor immune escape. However, the limited clinical efficacy of traditional IDO1 inhibitors has impeded their further development. Recently, apo-IDO1 that displace heme to target have been discovered, which exhibits slow dissociation rate reminiscent irreversible inhibitors. This characteristic suggests sustained engagement, offering pharmacodynamic advantage. Therefore, development emerges as promising strategy field IDO1-related...
Soybean continuous cropping (SC) leads to obstacles, and soil-borne fungal diseases occur frequently. Rotation can alleviate obstacles. However, the long-term effects of rotation on structure function community in soil are not clear. In this study, five systems, SC, fallow (CK), fallow-soybean (FS), corn-soybean (CS), wheat-soybean (WS), were implemented area soybean. After 13 years planting, high-throughput sequencing was used evaluate diversity communities study relationship between...
Taxonomic classification of three mitten crabs (Eriocheir sinensis, E. hepuensis, and japonica) has long been controversial. In this study, the complete mitogenomes were reported. The conserved in organization genes with 13 protein-coding genes, 2 rRNA 22 tRNA 1 control region. Nucleotide variations among identified both coding non-coding regions. addition, variable numbers tandem repeats region crabs. mitogenome sequences provide a valuable resource to elucidate taxonomic relationship...
The methylation of m6A (N6-position adenosine) has been found to be associated with inflammatory response. We hypothesize that modification plays a role in the inflammation airway epithelial cells during lung inflammation. However, precise changes and functions acute injury (ALI) are not well understood. Here we report METTL3 (methyltransferase-like 3)-mediated GATA6 (GATA-binding factor 6) mRNA inhibits ALI secretion proinflammatory cytokines cells. expression levels decrease tissues mice...
This study is aimed at increasing the accuracy of preimplantation genetic test for monogenic defects (PGT-M). We applied Bayesian statistics to optimize data analyses mutated allele revealed by sequencing with aneuploidy and linkage (MARSALA) method PGT-M. In doing so, we developed a algorithm incorporating PCR SNV detection genome around known mutation sites in order determine quantitatively probabilities having disease-carrying alleles from parents diseases. Both recombination events...
A high-fat diet (HFD) is known to lead obesity, and contributes the progression of non-alcoholic fatty liver disease. The objective this study was evaluate effects sleeve gastrectomy (SG) on HFD-induced hepatic steatosis.Fifteen 4-week-old, male Wistar rats were randomly assigned into three groups: NC, HFD + SHAM SG. Their body weight, glucose-lipid metabolism, inflammation indices, steatosis fibroblast growth factor 21 (FGF21) levels measured.Postoperatively, weights in SG group decreased...
Small nucleolar RNAs (snoRNAs) function in guiding 2′-O-methylation and pseudouridylation of ribosomal (rRNAs) small nuclear (snRNAs). In recent years, more snoRNAs have been found to play novel roles mRNA regulation, such as pre-mRNA splicing or RNA editing. our previous study, we a silkworm C/D box snoRNA Bm-15 can interact with Notch receptor gene vitro. To further study the Bm-15, cloned its homolog Sf-15 from Spodoptera frugiperda investigate Sf9 cells. We showed that knocking down...
Abstract Background Bone marrow (BM) and umbilical cord (UC) are the main sources of mesenchymal stem cells (MSCs). These two MSCs display significant differences in many biological characteristics, yet underlying regulation mechanisms these remain largely unknown. Results BMMSCs UCMSCs were isolated from inbred Wuzhishan miniature pigs first global DNA methylation gene expression profiles porcine generated. The osteogenic adipogenic differentiation ability is greater than that UCMSCs. A...