A5043306934- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- T-cell and B-cell Immunology
- Chromosomal and Genetic Variations
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- Genetic diversity and population structure
- Advanced Glycation End Products research
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Light effects on plants
- Plant Reproductive Biology
- RNA Research and Splicing
- Microbial Community Ecology and Physiology
- Inflammatory mediators and NSAID effects
- Pain Mechanisms and Treatments
- Genomics and Chromatin Dynamics
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Prenatal Screening and Diagnostics
- Eicosanoids and Hypertension Pharmacology
- Studies on Chitinases and Chitosanases
- Identification and Quantification in Food
National Institute on Aging
2020-2025
Peking University
2004-2024
Stomatology Hospital
2023
Guangxi Medical University
2023
Nanjing Normal University
2022
Ganzhou People's Hospital
2017-2020
University of Nebraska–Lincoln
2017
University of Kentucky
2016
Marymount University
2016
Changshu Institute of Technology
2015
Significance One missing or wrong nucleotide out of six billion in a human genome can cause genetic disease. Detecting such point mutation single germ cell has been daunting challenge vitro fertilization, yet one cannot afford to make any mistakes selecting viable embryo for transfer. Mutated allele revealed by sequencing with aneuploidy and linkage analyses (MARSALA) combines next-generation single-cell whole-genome amplification methodologies, allowing diagnosis single-molecule precision,...
The contribution of three-dimensional genome organization to physiological ageing is not well known. Here we show that large-scale chromatin reorganization distinguishes young and old bone marrow progenitor (pro-) B cells. These changes result in increased interactions at the compartment level reduced within topologically associated domains (TADs). gene encoding Ebf1, a key cell regulator, switches from A with age. Genetically reducing Ebf1 recapitulates some features pro-B TADs are most age...
Background Pyropia haitanensis and P. yezoensis are two economically important marine crops that also considered to be research models study the physiological ecology of intertidal seaweed communities, evolutionary biology plastids, origins sexual reproduction. This plastid genome information will facilitate breeding, population genetics phylogenetics. Principal Findings We have fully sequenced using next-generation sequencing circular genomes hatanensis (195,597 bp) (191,975 bp), largest...
The molecular mechanism of pollen germination and tube growth has been revealed in detail during the last decade, while that suspends grains a dormant state is largely unclear. Here, we identified JINGUBANG (JGB) gene by screening pollen-specific genes for those are unnecessary germination. We showed jgb loss-of-function mutant exhibited hyperactive sucrose-only medium inside anther, this phenotype was rescued transgenic expression JGB plants. contains seven WD40 repeats highly conserved...
Three-dimensional genome organization orchestrates recombination and transcription of immunoglobulin heavy chain (Igh) genes. The structure wild-type (WT) alleles includes a prominent architectural stripe that extends from cluster CTCF binding elements at the 3' end locus (3'CBE), suggesting interactions this with sequences throughout 2 Mb Igh TAD. Here we elucidate interplay between regulatory located in 3'Igh domain (260 kb) impact stripe. CTCF-lacking intronic enhancer, Eµ, promotes...
The chloroplast is essential for plant photosynthesis and production, but the regulatory mechanism of development still elusive. Here, a novel gene, WHITE TO GREEN1 (WTG1), was identified to have function in plastid gene expression by screening Arabidopsis leaf coloration mutants. WTG1 encodes chloroplast-localized tetratricopeptide repeat protein that expressed widely cells. Disruption suppresses growth, retards greening development, represses photosynthetic expression, complemented...
Different mechanisms govern the first and second steps of IgH gene recombination.
Abstract While pollen dormancy has been proposed to play a necessary role in sexual reproduction, it remains poorly understood. Here, we used traditional germination assays characterize dormancy. Our results underscore variation the degree of between individual grains. In addition, provide evidence that JINGUBANG (JGB), previously defined as negative regulator Arabidopsis (Arabidopsis thaliana), is responsible for uneven degrees dormancy, asynchronous vitro reflected varied expression levels...
The study aim was to determine how peripheral trigeminal nerve injury affects mitochondrial respiration and test efficacy of combined treatment with 2 Federal Drug Administration approved drugs potential for improving bioenergetics, pain anxiety-related behaviors in a chronic orofacial neuropathic mouse model.Efficacy (R)-(+)-4-amino-3-isoxazolidinone (D-cycloserine, DCS), an N-Methyl-D-aspartate antagonist/agonist, Pioglitazone (PIO), selective agonist nuclear receptor peroxisome...
Aims-To determine whether herpes simplex virus-based vectors can efficiently transduce mouse trigeminal ganglion (TG) neurons and attenuate preexisting nerve injury-induced whisker pad mechanical hypersensitivity in a inflammatory compression (TIC) neuropathic pain model.Methods-Tissue transduction efficiencies of replication-conditional replication-defective to pads after topical administration subcutaneous injection were assessed using quantitative real-time PCR (qPCR).Tissue tropism...
The rearrangement and expression of the immunoglobulin μ heavy chain (Igh) gene require communication intragenic Eμ 3′ regulatory region (RR) enhancers with variable (VH) promoter. binding transcription factor YY1 has been implicated in enhancer-promoter communication, but protein network remains obscure. By analyzing comprehensive proteome 1-kb wild-type enhancer that lacking site, we identified male-specific lethal (MSL)/MOF complex as a component network. We found MSL2 recruitment depends...
Mechanical compression is important in disc degeneration. N-cadherin (N-CDH)-mediated signaling contributes to the maintenance of normal nucleus pulposus (NP) cell phenotype and NP matrix biosynthesis. Our preliminary study demonstrated that a high‑magnitude (20% deformation) promotes senescence three‑dimensional scaffold culture system. The aim present was investigate whether N‑CDH‑mediated alleviates under above‑mentioned compression. cells were transfected with recombinant lentiviral...
Maintaining the appropriate number of mitochondrial DNA (mtDNA) molecules is crucial for supporting metabolism and function in both plant animal cells. For example, a substantial decrease mtDNA levels occurs as key part pollen development. The molecular mechanisms regulating copy are largely unclear, particularly with regard to those that reduce levels. Here, we identified purified 20-kD endonuclease, M20, from maize (Zea mays) mitochondria. We found M20 be an His-Asn-His/Asn (H-N-H/N)...
Plant cells contain only small amounts of mitochondrial DNA (mtDNA), with the genomic information shared among multiple mitochondria. The biological relevance and molecular mechanism underlying this hallmark plant has been unclear. Here, we report that Arabidopsis thaliana plants exhibited significantly reduced growth dysfunction when mtDNA copy number was increased to degree each mitochondrion possessed DNA. mitochondrion-encoded transcripts several fold in presence elevated levels....
Background Despite poor oral hygiene, the Baiku Yao (BKY) ethnic group in China presents a low prevalence of dental caries, which may be related to genetic susceptibility. Due strict intra-ethnic marriage rule, this has an advantage studying interaction between factors and other regulatory caries.