A5012157159- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Natural product bioactivities and synthesis
- Microbial Natural Products and Biosynthesis
- Ginkgo biloba and Cashew Applications
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Neurological Disease Mechanisms and Treatments
- Photosynthetic Processes and Mechanisms
- Pharmacological Effects of Natural Compounds
- Malaria Research and Control
- Plant Pathogens and Fungal Diseases
- Traditional Chinese Medicine Analysis
- Phytochemicals and Antioxidant Activities
- Plant and Fungal Species Descriptions
- Essential Oils and Antimicrobial Activity
- Phytochemistry and Biological Activities
- Insect Resistance and Genetics
- Metabolomics and Mass Spectrometry Studies
- Ginseng Biological Effects and Applications
- Plant-Microbe Interactions and Immunity
- Computational Drug Discovery Methods
- Genetic and Environmental Crop Studies
- Data Quality and Management
China Pharmaceutical University
2016-2025
State Key Laboratory of Natural Medicine
2025
Hainan University
2022-2024
Sanya University
2024
Minzu University of China
2021-2023
South China Normal University
2008-2023
First Affiliated Hospital of Zhengzhou University
2022
State Ethnic Affairs Commission
2021-2022
University of California, Berkeley
2022
Joint BioEnergy Institute
2022
Jianquan Liu and colleagues report the draft genome sequence of domestic yak, Bos grunniens. Their comparative analyses with low-altitude cattle provide insights into high-altitude adaptation in yak. Domestic yaks (Bos grunniens) meat other necessities for Tibetans living at high altitude on Qinghai-Tibetan Plateau adjacent regions. Comparison between yak closely related taurus) is informative studying animal to altitude. Here, we present a female generated using Illumina-based technology...
Summary Six transcription factors of APETALA2/ethylene‐response factor ( AP 2/ ERF ) family were cloned and analyzed in A rtemisia annua . Real‐time quantitative polymerase chain reaction RT ‐ Q PCR showed that Aa ORA exhibited similar expression patterns to those amorpha‐4,11‐diene synthase gene ADS ), cytochrome P450‐dependent hydroxylase CYP71AV1 double bond reductase 2 DBR2 different tissues a is trichome‐specific factor, which expressed both glandular secretory trichomes GST s)...
The plant Artemisia annua is well known due to the production of artemisinin, a sesquiterpene lactone that widely used in malaria treatment. Phytohormones play important roles secondary metabolism, such as jasmonic acid (JA), which can induce artemisinin biosynthesis A. annua. Nevertheless, JA-inducing mechanism remains poorly understood. expression gene AaMYC2 was rapidly induced by JA and binds G-box-like motifs within promoters CYP71AV1 DBR2, are key structural genes biosynthetic pathway....
Summary Artemisinin is a type of sesquiterpene lactone well known as an antimalarial drug, and specifically produced in glandular trichomes Artemisia annua . However, the regulatory network for artemisinin biosynthetic pathway remains poorly understood. Exploration trichome‐specific transcription factors would facilitate elucidation mechanism biosynthesis. The WRKY factor GLANDULAR TRICHOME ‐ SPECIFIC 1 ( Aa GSW ) was cloned analysed A. exhibited similar expression patterns to genes AP 2/...
Photosynthesis is an important plant metabolic mechanism that improves carbon absorption and crop yield. Photosynthetic efficiency greatly hampered by cold stress (CS). Melatonin (ME) a new growth regulator regulates wide range of abiotic responses. However, the molecular ME-mediated photosynthetic regulation in cold-stressed plants not well understood. Our findings suggest under low-temperature (15/5 °C for 7 days), spraying with ME (200 µM) enhanced gas exchange characteristics pigment...
Abstract Citrus reticulata cv. Chachiensis (CRC) is an important medicinal plant, its dried mature peels named “Guangchenpi”, has been used as a traditional Chinese medicine to treat cough, indigestion, and lung diseases for several hundred years. However, the biosynthesis of crucial natural products polymethoxylated flavonoids (PMFs) in CRC remains unclear. Here, we report chromosome-scale genome assembly with size 314.96 Mb contig N50 16.22 Mb. Using multi-omics resources, discover...
Ginkgolides are secondary metabolites unique to Ginkgo biloba with the potential prevent and treat cardiovascular cerebrovascular diseases. Although biosynthetic pathways of ginkgolides have been partly uncovered, mechanism regulating their biosynthesis is still largely unknown. Here, using multiomic genetic analyses, we report identification a transcription factor, named ETHYLENE RESPONSE FACTOR ASSOCIATED WITH GINKGOLIDE BIOSYNTHESIS (GbEAG), as critical regulator ginkgolide biosynthesis....
There are many biosynthetic pathways competing for the metabolic flux with artemisinin pathway in Artemisia annua L. To study relationship between genes encoding enzymes at branching points and pathway, β-caryophyllene, β-farnesene squalene were sprayed on young seedlings of A. . Transient expression assays indicated that transcription levels β-caryophyllene synthase ( CPS ), BFS ) SQS inhibited by squalene, respectively, while some increased. Thus, inhibition these may be helpful to improve...
Abstract A matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) assisted genome mining strategy was developed for the discovery of glycosyltransferase (GT) from root Platycodon grandiflorum . di‐ O ‐glycosyltransferase PgGT1 discovered and characterized that is capable catalyzing platycoside E (PE) synthesis through attachment two β‐1,6‐linked glucosyl residues sequentially to residue at C3 position platycodin D (PD). Although UDP‐glucose preferred sugar donor...
Abstract Tetraploid Artemisia annua plants were successfully inducted by using colchicine, and their ploidy was confirmed flow cytometry. Higher stomatal length but lower frequency in tetraploids revealed could be considered as indicators of polyploidy. The average level artemisinin increased from 39% to 56% than that the diploids during vegetation period, detected high‐performance liquid chromatography–evaporative light scattering detector. Gene expressions 10 key enzymes related...
Finding an efficient and affordable treatment against malaria is still a challenge for medicine. Artemisinin effective anti-malarial drug isolated from Artemisia annua. However, the artemisinin content of A. annua very low. We used transgenic technology to increase by overexpressing cytochrome P450 monooxygenase (cyp71av1) reductase (cpr) genes. CYP71AV1 key enzyme in biosynthesis pathway, while CPR redox partner CYP71AV1. Eight independent plants were obtained through Agrobacterium...
The phytohormone abscisic acid (ABA) plays an important role in plant development and environmental stress response. In this study, we cloned ABA receptor orthologue, AaPYL9, from Artemisia annua L. AaPYL9 is expressed highly leaf flower. protein can be localized both nucleus cytoplasm. Yeast two-hybrid assay shows specifically interact with AtABI1 but not AtABI2, AtHAB1 or AtHAB2. enhance the interaction between while AaPYL9-89 Pro→Ser AaPYL9-116 His→Ala point mutations abolishes...
As a medicinal plant, the resource of
Summary Lonicera macranthoides (LM) and L. japonica (LJ) are medicinal plants widely used in treating viral diseases, such as COVID‐19. Although the two species morphologically similar, their secondary metabolite profiles significantly different. Here, metabolomics analysis showed that LM contained ~86.01 mg/g hederagenin‐based saponins, 2000‐fold higher than LJ. To gain molecular insights into its production, a chromosome‐level genome of was constructed, comprising 9 pseudo‐chromosomes with...
Plants are sessile organisms, and they can not move away under abiotic or biotic stresses. Thus plants have evolved a set of genes that response to adverse environment modulate gene expression. In this study, we characterized functionally studied an ERF transcription factor from Artemisia annua, AaERF1, which plays important role in stress responses. The AaERF1 promoter had been cloned GUS staining results promoter-GUS transgenic A. annua showed is expressed ubiquitiously all organs. Several...
Patients with biliary tract cancer (BTC) were associated poor prognosis and limited therapeutic options after first-line therapy currently. In this study, we sought to evaluate the feasibility tolerability of sintilimab plus anlotinib as second-line treatment for patients advanced BTC. Eligible had histologically confirmed locally unresectable or metastatic BTC failed recruited. The primary endpoint was overall survival (OS). Simultaneously, association between clinical outcomes genomic...