A5036858705- Esophageal Cancer Research and Treatment
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- Virus-based gene therapy research
- Cancer-related gene regulation
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- RNA Research and Splicing
- MicroRNA in disease regulation
- S100 Proteins and Annexins
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Screening and Detection
- Biomarkers in Disease Mechanisms
- Cancer Research and Treatments
- Gastrointestinal Tumor Research and Treatment
- Cytokine Signaling Pathways and Interactions
- Lung Cancer Diagnosis and Treatment
- Cancer Mechanisms and Therapy
First Affiliated Hospital of Zhengzhou University
2016-2025
Zhengzhou University
2005-2024
Henan Cancer Hospital
2018-2024
Zero to Three
2023
Queen Mary University of London
2011
Interleukin-12 (IL-12) has emerged as one of the most potent agents for anti-tumor immunotherapy. However, potentially lethal toxicity associated with systemic administration IL-12 precludes its clinical application. Here we redesign molecule in such a way that efficacy is not compromised, but toxic effects are eliminated. Deletion N-terminal signal peptide can effect change by preventing secretion from cells. We use newly designed tumor-targeted oncolytic adenovirus (Ad-TD) to deliver...
Evidences have shown that lncRNAs involve in the initiation and progression of various cancers including esophageal squamous cell carcinoma (ESCC). The aberrant expression lncRNA MALAT1 was investigated 106 paired ESCC tissues adjacent non-cancerous by qRT-PCR. Down-regulated Ezh2 over-expression plasmid were constructed respectively to analyze β-catenin, Lin28 genes. We found level higher human (P=0.0011), which closely correlated with WHO grade (P=0.0395, P=0.0331), lymph node metastasis...
Current diagnosis of glioma types requires combining both histological features and molecular characteristics, which is an expensive time-consuming procedure. Determining the tumor directly from whole-slide images (WSIs) great value for diagnosis. This study presents integrated model automatic classification diffuse gliomas annotation-free standard WSIs. Our developed on a training cohort (n = 1362) validation 340), tested internal testing 289) two external cohorts 305 328, respectively)....
Pancreatic cancer (PC) is a highly lethal human cancer. We previously found that Serine protease 3 (PRSS3), as an oncogene, significantly upregulated in PC. In this study, we aimed to investigate the potential mechanism of PRSS3 dysregulation research, low miR-217 and high circ-ADAM9 expression were PC tissues cell lines, which was closely associated with advanced clinical stage lymph node metastasis. Patients or had shorter survival time than those expression. Functionally, manipulation...
ROS1 gene rearrangements have been reported in diverse cancer types including non-small-cell lung (NSCLC), and with a notably higher prevalence adenocarcinoma. The tyrosine kinase inhibitors, crizotinib, lorlatinib, entrectinib, demonstrated favorable efficacy treating ROS1-rearranged NSCLCs. Herein, we retrospectively reviewed 17 158 NSCLC patients whose tumor specimen and/or circulating cell-free DNA underwent comprehensive genomic profiling. A total of 258 unique were identified...
Abstract Background Complex kinase rearrangement, a mutational process involving one or two chromosomes with clustered rearrangement breakpoints, interferes the accurate detection of fusions by DNA-based next-generation sequencing (NGS). We investigated characteristics complex ALK rearrangements in non-small cell lung cancers using multiple molecular tests. Methods Samples cancer patients were analyzed targeted-capture NGS probes tilling selected intronic regions fusion partner genes,...
Patients with biliary tract cancer (BTC) were associated poor prognosis and limited therapeutic options after first-line therapy currently. In this study, we sought to evaluate the feasibility tolerability of sintilimab plus anlotinib as second-line treatment for patients advanced BTC. Eligible had histologically confirmed locally unresectable or metastatic BTC failed recruited. The primary endpoint was overall survival (OS). Simultaneously, association between clinical outcomes genomic...
Abstract Aims Although radiotherapy is a core treatment modality for various human cancers, including glioblastoma multiforme (GBM), its clinical effects are often limited by radioresistance. The specific molecular mechanisms underlying radioresistance largely unknown, and the reduction of an unresolved challenge in GBM research. Methods We analyzed verified expression nuclear autoantigenic sperm protein (NASP) gliomas relationship with patient prognosis. also explored function NASP cell...
Background/Aims: This study aims to examine the effect of long noncoding RNA HOST2 (LncRNA HOST2) on epithelial-mesenchymal transition (EMT), proliferation, invasion and migration hepatocellular carcinoma (HCC) cells via activation JAK2-STAT3 signaling pathway. Methods: HCC para-cancerous tissues were collected from 136 patients. Immunohistochemistry was used detect expression JAK2 STAT3. SMMC7721 grouped into blank, negative control (NC), mimic inhibitor groups. The mRNA protein levels...
Pancreatic cancer is a lethal malignancy with poor prognosis. This study aims to identify pancreatic cancer-related genes and develop robust diagnostic model detect this disease. Weighted gene co-expression network analysis (WGCNA) was used determine potential hub for cancer. Their mRNA protein expression levels were validated through reverse transcription PCR (RT-PCR) immunohistochemical (IHC). Diagnostic models developed by eight machine learning algorithms ten-fold cross-validation. Four...
Background Immune checkpoint blockade (ICB)-based immunotherapy has significantly improved survival in advanced melanoma. However, many patients exhibit resistance to these therapies. This study examines the impact of BTLA promoter methylation on its expression, immune cell infiltration, and clinical outcomes, evaluating potential as a prognostic predictive biomarker for response. Methods We analyzed gene expression data from public datasets (The Cancer Genome Atlas (TCGA), Gene Expression...
Human mutT homolog 1(MTH1), the oxidized dNTP pool sanitizer enzyme, has been reported to be highly expressed in various malignant tumors. However, oncogenic role of MTH1 gastric cancer remains determined. In current study, we found that was overexpressed human tissues and cells. Using an vitro inhibitor screening system, compounds available our laboratory were screened small molecules containing 5-cyano-6-phenylpyrimidine structure firstly show potently specifically inhibitory effect on...
The changes in cancer cell surface molecules and intracellular signaling pathways during tumorigenesis make delivery of adenovirus-based therapies inefficient. Here we have identified carcinoembryonic antigen- related adhesion molecule 6 (CEACAM6) as a cellular protein that restricts the ability adenoviral vectors to infect cells. We demonstrated CEACAM6 can antagonize Src pathway, downregulate cytoskeleton proteins, block adenovirus trafficking nucleus human pancreatic Similar...
Oncolytic adenoviruses based on serotype 5 (Ad5) have several shortcomings, including the downregulation of its receptor in cancer cells, high prevalence neutralizing antibodies and hepatotoxicity. Another adenoviral serotype, Ad11, could overcome these obstacles. Here, we show that human cell lines express higher levels Ad11 CD46, resulting much better infectivity than Ad5. Surprisingly, only 36% (9/25) were more sensitive to Ad11- Ad5-mediated cytotoxicity. Investigations revealed it was...
Esophageal cancer (EC) is an aggressive malignancy and the most common solid tumor of gastrointestinal tract all over world, with high incidence in Asia. The current study was designed to investigate anticancer efficacy mechanism that involved action a natural ent-kaurene diterpenoid, JDA-202, targeting EC.We found antioxidant protein peroxiredoxin I (Prx I) upregulated human EC tissues as well cell lines. novel compound isolated from Isodon rubescens (Labiatae), proved possess strong...
Aberrant activation of the canonical WNT or WNT/β-catenin signaling pathway plays a pivotal role in multiple types cancers. WNT5A, nontransforming protein suppressing Wnt/β-catenin pathway, is frequently detected to be hypermethylated colorectal cancer (CRC). In this study, we investigated prognostic value WNT5A methylation human patients and its potential underlying molecular mechanisms cultured CRC cells.We measured mRNA level using qRT-PCR DNA methylation-specific PCR (MSP) HCT116, HT29,...
Aberrantly upregulated expression of selected members annexin, a group calcium- and membrane-binding proteins, have been found to be associated with metastasis, poor prognosis, other clinical characteristics in colorectal cancer (CRC), the third most diagnosed cancer. However, ANXA13 (encoding protein annexin A13), original founder gene A family, has not studied carefully as potential prognostic biomarker CRC.The level A13 was determined by western blot panel CRC cell lines. Tumor invasion...
Tumor metastasis is a significant obstacle to curing colorectal cancer (CRC). C/EBPβ thought play an important role in CRC invasion and metastasis. In this study, we assessed whether C/EBPβ-mediated tumor was dependent on MMP3, the expression of which upregulated by C/EBPβ. We then determined upregulation associated with MMP3 levels metastatic status human patients.A total 102 patients were recruited for study. mRNA protein cell lines patient specimens reverse transcription-polymerase chain...
Abstract Background CircRNAs with tissue-specific expression and stable structure may be good tumor prognostic markers. However, the of circRNAs in esophageal squamous cell carcinoma (ESCC) remain unknown. We aim to identify construct a circRNA-related signature ESCC. Methods RNA sequencing was used test circRNA profiles 73 paired ESCC normal tissues after RNase R enrichment. Bioinformatics methods, such as principal component analysis (PCA), t-distributed Stochastic Neighbor Embedding...
Aim: In gliomas, mutations in the core promoter region of telomerase reverse transcriptase (TERT) gene have been associated with specific subtypes and are inversely correlated IDH1 mutation status, predicting poor prognosis. Thus, TERT status might be a candidate for development as prognostic biomarker. However, current detection methods using conventional polymerase chain reaction (PCR), followed by Sanger sequencing, low sensitivity time-consuming. To improve test efficacy, we developed...
Gastric plexiform fibromyxoma (PF) is a rare mesenchymal tumor with histologically distinctive multinodular pattern, dissimilar to conventional gastrointestinal stromal (GIST). The current study presents one case of gastric PF, and GIST platelet-derived growth factor receptor α (PDGFRA) mutation mimicking discusses their differential diagnoses. two patients were 51-year-old male PF 47-year-old female GIST, each whom presented an occupying lesion in the antrum. Histologically, cases shared...