A5055653838- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Angiogenesis and VEGF in Cancer
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related Molecular Pathways
- Cancer, Lipids, and Metabolism
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Eicosanoids and Hypertension Pharmacology
- Chromium effects and bioremediation
- High Altitude and Hypoxia
- Cancer Mechanisms and Therapy
- Autophagy in Disease and Therapy
- Nitric Oxide and Endothelin Effects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced biosensing and bioanalysis techniques
- Ubiquitin and proteasome pathways
- Adipose Tissue and Metabolism
- Nanoplatforms for cancer theranostics
- Immune cells in cancer
- Cancer Treatment and Pharmacology
Zhengzhou University
2019-2025
Lanzhou University of Technology
2024-2025
Henan Cancer Hospital
2022-2025
Third Affiliated Hospital of Zhengzhou University
2024-2025
Henan Academy of Sciences
2020-2025
Utah Tech University
2025
Thomas Jefferson University
2015-2024
First Affiliated Hospital of Zhengzhou University
2019-2024
First Affiliated Hospital of Wenzhou Medical University
2024
Nanjing Drum Tower Hospital
2018-2024
Hypoxia-inducible factor 1 (HIF-1) is found in mammalian cells cultured under reduced O2 tension and necessary for transcriptional activation mediated by the erythropoietin gene enhancer hypoxic cells. We show that both HIF-1 subunits are basic-helix-loop-helix proteins containing a PAS domain, defined its presence Drosophila Per Sim ARNT AHR proteins. alpha most closely related to Sim. beta series of products, which can thus heterodimerize with either or AHR. (ARNT) RNA protein levels were...
Expression of vascular endothelial growth factor (VEGF) is induced in cells exposed to hypoxia or ischemia.Neovascularization stimulated by VEGF occurs several important clinical contexts, including myocardial ischemia, retinal disease, and tumor growth.Hypoxia-inducible 1 (HIF-1) a heterodimeric basic helix-loop-helix protein that activates transcription the human erythropoietin gene hypoxic cells.Here we demonstrate involvement HIF-1 activation transcription.VEGF 5-flanking sequences...
Hypoxia-inducible factor 1 (HIF-1) is a basic helix-loop-helix transcription which expressed when mammalian cells are subjected to hypoxia and activates of genes encoding erythropoietin, vascular endothelial growth factor, other proteins that important for maintaining oxygen homeostasis. Previous studies have provided indirect evidence HIF-1 also regulates glycolytic enzymes. In this paper we characterize response elements in the promoters ALDA, ENO1, Ldha genes. We demonstrate plays an...
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic helix-loop-helix protein implicated in the transcriptional activation of genes encoding erythropoietin, glycolytic enzymes, and vascular endothelial growth hypoxic mammalian cells. In this study, we have quantitated HIF-1 DNA-binding activity levels alpha beta subunits human HeLa cells exposed to O2 concentrations ranging from 0 20% absence or presence mM KCN inhibit oxidative phosphorylation cellular consumption. activity, each...
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic helix-loop-helix transcription that regulates hypoxia-inducible genes including the human erythropoietin (EPO) gene. In this study, we report structural features of HIF-1α subunit are required for heterodimerization, DNA binding, and transactivation. The HIF-1β (ARNT; aryl hydrocarbon receptor nuclear translocator) subunits were coimmunoprecipitated from extracts, indicating these proteins heterodimerize in absence DNA....
Exposure of rats to hypoxia (7% O2) markedly increased the level heme oxygenase-1 (HO-1) mRNA in several tissues. Accumulation HO-1 transcripts was also observed after exposure rat aortic vascular smooth muscle (VSM) cells 1% O2, and this induction dependent on gene transcription. Activation mouse by all agents thus far tested is mediated two 5′-enhancer sequences, SX2 AB1, but neither fragment responsive VSM cells. Hypoxia-dependent chloramphenicol acetyltransferase (CAT) reporter a 163-bp...
Hypoxia-inducible factor 1 (HIF-1) binds to cis-acting hypoxia-response elements within the erythropoietin, vascular endothelial growth factor, and other genes activate transcription in hypoxic cells. HIF-1 is a basic helix-loop-helix composed of HIF-1alpha HIF-1beta subunits. Here, we demonstrate that contains two transactivation domains located between amino acids 531 826. When expressed as GAL4 fusion proteins, transcriptional activity these increased response hypoxia. Fusion protein...
Phosphatidylinositol 3-kinase (PI 3-kinase) is a signaling molecule that controls numerous cellular properties and activities. The oncogene v- p3k homolog of the gene coding for catalytic subunit PI 3-kinase, p110α. P3k induces transformation cells in culture, formation hemangiosarcomas young chickens, myogenic differentiation myoblasts. Here, we describe role angiogenesis. Overexpression v-P3k protein or equipped with myristylation signal, Myr-P3k, can induce angiogenesis chorioallantoic...
Abstract Reactive oxygen species (ROS) are associated with multiple cellular functions such as cell proliferation, differentiation, and apoptosis. However, the direct roles of endogenous ROS production still remain to be elucidated. In this study, we found that high levels were spontaneously produced by ovarian prostate cancer cells. This elevated was inhibited NADPH oxidase inhibitor diphenylene iodonium (DPI) mitochondria electron chain rotenone in To further analyze source production,...
MicroRNAs (miRNAs) are endogenous, small noncoding RNAs that play important roles in various cellular functions and tumor development. Recent studies have indicated miR-21 is one of the miRNAs associated with growth metastasis, but role molecular mechanism regulating angiogenesis remain to be elucidated. In this study, was overexpressed by transfecting pre-miR-21 into human prostate cancer cells assayed using chicken chorioallantoic membrane (CAM). We found overexpression DU145 increased...
Epidemiological data suggest that consumption of fruits and vegetables has been associated with a lower incidence cancer. Cyanidin-3-glucoside (C3G), compound found in blackberry other food products, was shown to possess chemopreventive chemotherapeutic activity the present study. In cultured JB6 cells, C3G able scavenge ultraviolet B-induced ·OH O2_˙ radicals. vivo studies indicated treatment decreased number non-malignant malignant skin tumors per mouse induced by...
Abstract Purpose: To investigate expression, regulation, potential role and targets of miR-195 miR-497 in breast cancer. Experimental Design: The expression patterns were initially examined cancer tissues cell lines by Northern blotting quantitative real-time PCR. Combined bisulfite restriction analysis sequencing carried out to study the DNA methylation status genes. Breast cells stably expressing established their targets. Finally, normal, fibroadenoma employed analyze correlation between...
MiR-145 can regulate cell apoptosis, proliferation, neural development and stem differentiation. Previous studies indicate that miR-145 is downregulated in human colon cancer cells. However, the molecular mechanisms of used to carcinogenesis angiogenesis remain be clarified. Here, we show expression ovarian tissues lines. inhibits p70S6K1 post-transcriptional by binding its 3'-UTR. The angiogenic factors hypoxia-inducible factor 1 (HIF-1) vascular endothelial growth (VEGF), which are...
Dysregulation of microRNAs is a common feature in human cancers, including breast cancer (BC). Here we describe the epigenetic regulation miR-148a and miR-152 their impact on BC cells. Due to hypermethylation CpG island, expression levels both (miR-148a/152) are decreased tissues DNMT1, DNA methyltransferase 1 for maintenance methylation, aberrantly up-regulated its overexpression responsible promoters. Intriguingly, found that DNMT1 expression, which one targets miR-148a/152, inversely...
Cancer cells have an imbalance in oxidation-reduction (redox) homeostasis. Understanding the precise mechanisms and impact of altered redox microenvironment on immunologic reaction to tumors is limited.We isolated exosomes from ovarian cancer through ultracentrifuge characterized by Western-blots Nanoparticle Tracking Analysis. 2D, 3D-coculture tumor model, 3D live cell imaging were used study interactions between cells, macrophages CD3 T vitro. The role exosomal miR-155-5p growth was...