A5017255882- MicroRNA in disease regulation
- TGF-β signaling in diseases
- Bone Metabolism and Diseases
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Wnt/β-catenin signaling in development and cancer
- Liver Disease Diagnosis and Treatment
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Cardiac Valve Diseases and Treatments
- RNA Research and Splicing
- S100 Proteins and Annexins
- Connective Tissue Growth Factor Research
- Renal and related cancers
- Cytokine Signaling Pathways and Interactions
- Cancer Cells and Metastasis
- Infective Endocarditis Diagnosis and Management
- RNA Interference and Gene Delivery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Biomarkers in Disease Mechanisms
- Bone health and treatments
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Liver physiology and pathology
- Bone and Dental Protein Studies
Chongqing Medical University
2014-2023
Children's Hospital of Chongqing Medical University
2008-2023
Neijiang Normal University
2021
Southwest Medical University
2021
Ministry of Education of the People's Republic of China
2013-2015
Liver plays an essential role in regulating lipid metabolism, and chronically disturbed hepatic metabolism may cause obesity metabolic syndrome, which lead to non-alcoholic fatty liver disease (NAFLD). Increasing evidence indicates long non-coding RNAs (lncRNAs) play important energy metabolism. Here, we investigated the of lncRNA H19 its potential association with NAFLD. We found that was up-regulated oleic acid-induced steatosis during development high-fat diet (HFD)-induced Exogenous...
Background and Objective S100A8 S100A9, two members of the S100 protein family, have been reported in association with tumor cell differentiation progression. Previous study has showed that their expression stromal cells colorectal carcinoma (CRC) is associated size. Here, we investigated clinical significances S100A9 CRC underlying molecular mechanisms. Methods Expression matching distal normal tissues were measured by reverse transcriptase polymerase chain reaction (RT-PCR),...
Mutation of the tumor suppressor TP53 gene occurs in greater than half all human cancers. In addition to loss function wild-type TP53, gain-of-function mutations endow cancer cells with more malignant properties. R273 is a mutation hotspot p.R273H, p.R273C, and p.R273G variants occurring most commonly patient samples. To better understand consequences these mutations, we constructed cell lines expressing or explored their characteristics. We found that p.R273H but not p.R273G, enhanced...
Abstract Wnt/β‐catenin pathway plays an important role in regulating embryonic development. Hepatocytes differentiate from endoderm during Hepatic progenitor cells (HPCs) have been isolated fetal liver and extrahepatic tissues. Most current studies development hepatic differentiation focused on Wnts, β‐catenin, their receptors. Here, we sought to determine the of Wnt antagonists liver‐derived HPCs. Using mouse tissues derived day E12.5 postnatal (PD) 28, found that 13 19 genes almost all...
Bone morphogenetic protein 9 (BMP9), a member of TGF-β superfamily, is reported to inhibit the growth and migration prostate cancer, osteosarcoma triple-negative MDA-MB-231 breast cancer cells. However, little known about effect on biological behaviors HER2-positive SK-BR-3 cells underlying mechanisms. This study aimed investigate effects BMP9 proliferation metastasis with over-expression or down-regulated expression. Results indicated that exogenously expressed inhibited while decreased...
Periodontal ligament stem cells (PDLSCs) with bone morphogenic ability are used to treat diseases such as periodontitis. Their treatment potential is increased when in combination proteins that induce osteogenic differentiation. For example, morphogenetic protein-9 (BMP9) has been found have potent activity. In the present study, PDLSCs were isolated from human periodontal membrane and infected recombinant adenoviruses expressing BMP9 (Ad-BMP9). Levels of markers runt-related transcription...
BMP9 induces both osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs). Nell1 is a secretory glycoprotein with osteoinductive anti-adipogenic activities. We investigated the role in BMP9-induced osteogenesis adipogenesis MSCs.Previously characterized MSCs iMEFs were used. Overexpression NELL1 or silencing mouse was mediated by adenoviral vectors. Early late markers assessed staining techniques qPCR analysis. In vivo activity an ectopic bone formation model athymic...
Previous studies have indicated that bone morphogenetic protein 9 (BMP9) can promote the osteogenic differentiation of mesenchymal stem cells (MSCs) and increase formation in diseases. However, mechanisms involved remained poorly understood. It is necessary to investigate specific regulatory were induced by BMP9. During process BMP9, expression microRNA-155 (miR-155) exhibited a tendency increasing at first then decreasing, which made us consider miR-155 may modulatory role this process, but...
Osteosarcoma (OS) is the most common primary malignant bone tumor in young patients. However, treatment paradigms and survival rates have not improved decades. MicroRNAs been shown to be critical regulators of physiological homeostasis pathological process, including disease. Nearly half microRNA (miRNA) genes are located at genomic regions fragile sites known frequently deleted or amplified various kinds cancers. In this study, we investigated role miR-30a OS. A negative correlation between...
Mesenchymal stem cells (MSCs) are multipotent progenitors that can undergo osteogenic differentiation under proper stimuli. We demonstrated BMP9 is one of the most BMPs. However, molecular mechanism underlying BMP9-initiated signaling in MSCs remains unclear. Through gene expression profiling analysis we identified several candidate mediators signaling. Here, focus on such mediator and investigate functional role cysteine-rich with EGF-like domains 2 (Creld2) Creld2 was originally as an ER...
The S100A9 protein, a member of the S100 protein family, is often upregulated in various types cancer, including hepatocellular carcinoma (HCC). acts as danger signal when secreted to extracellular space and thought play an important role during tumorigenesis. Despite this fact, little known about effects tumor microenvironment on HCC. Therefore, study, we investigated exogenous proliferation invasion HepG2 HCC cells, well molecular mechanisms underlying these effects. Our results...
Mesenchymal progenitor stem cells (MPCs) are a group of bone marrow stromal processing osteogenic, chondrogenic, adipogenic and myogenic lineages differentiations.Previous studies have demonstrated that morphogeneic protein 9(BMP9) is one the most osteogenic BMPs both in vitro vivo, however, underlying molecular mechanism osteogenesis induced by BMP9 needed to be deep explored.Here, we used recombinant adenoviruses assay introduce into C3H10T1/2 mesenchymal elucidate role CXCL12/CXCR4 signal...
MicroRNAs (miRNAs) are small non-coding RNAs, which important in the development of multidrug resistance cancer by regulating gene expression at post‑transcriptional level. The present study investigated functional effects miR‑522 chemoresistant colon cells. results demonstrated that was significantly downregulated doxorubicin (DOX) resistant cell line, HT29/DOX, compared with parental HT29 line. Overexpression HT29/DOX cells partially restored DOX sensitivity. miRNA target prediction...
Mesenchymal stem cells (MSCs) are multipotent and capable of differentiating into multiple cell types including osteoblastic, chondrogenic adipogenic lineages. We previously identified BMP9 as one the most potent BMPs that induce osteoblastic differentiation MSCs although exact molecular mechanism through which regulates osteogenic remains to be fully understood. Here, we seek develop a recombinant adenovirus system optimally silence mouse then characterize important role in MSCs. Using two...
Bone morphogenetic proteins (BMPs), particularly BMP9, have been shown to promote the osteogenic differentiation of murine multilineage cells (MMCs) and bone formation in diseases; however, mechanisms involved remain poorly understood. MicroRNAs (miRNAs or miRs) proven regulate mesenchymal stem cell (MSC) differentiation. In this study, we identified a novel mechanism that unravels functional axis key miRNA (miR-21) which contributes BMP9‑induced We screened differentially expressed miRNAs...
Cervical cancer is the second most common gynecological worldwide and remains one of leading causes cancer-associated mortality among women. S100A6 has been reported to be associated with development many types cancer. The aim present study was investigate effect on proliferation, apoptosis migration cervical cells its underlying molecular mechanisms. Quantative polymerase chain reaction (qPCR) used detect basic mRNA level in HeLa, SiHa CaSki cells. Western blot analysis protein S100A6,...
Liver has numerous critical metabolic functions including lipid metabolism, which is usually dysregulated in obesity, the syndrome, and non-alcoholic fatty liver disease (NAFLD). Increasing evidence indicates bone morphogenetic proteins (BMPs) play an important role adipogenesis thermogenic balance adipogenic progenitors adipose tissue. However, direct impact of BMPs on hepatic steatosis possible association with NAFLD are poorly understood. Here, we found that BMP4 was up-regulated oleic...
Effective and safe liver-directed gene therapy has great promise in treating a broad range of liver diseases. While adenoviral (Ad) vectors have been widely used for efficacious vivo delivery, their translational utilities are severely limited due to the short duration transgene expression solicitation host immune response. Used as promising polymeric vehicle drug release nucleic acid carboxymethyl chitosan (CMC) is biocompatible, biodegradable, anti-microbial, inexpensive, easy accessible....
Transforming growth factor-β (TGF-β) is known to promote tumor migration and invasion. Bone morphogenetic proteins (BMPs) are members of the TGF-β family expressed in a variety human carcinoma cell lines. The role bone protein 9 (BMP9), most powerful osteogenic factor, osteosarcoma (OS) progression has not been fully clarified. expression BMP9 its receptors OS lines was analyzed by RT-PCR. We found that were further investigated influence on biological behaviors cells. overexpression 143B...