A5010037435- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Plant Pathogens and Fungal Diseases
- Horticultural and Viticultural Research
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Postharvest Quality and Shelf Life Management
- Phytochemicals and Antioxidant Activities
- Plant Pathogenic Bacteria Studies
- Molecular Biology Techniques and Applications
- Biochemical and biochemical processes
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Fermentation and Sensory Analysis
- Plant Disease Resistance and Genetics
- Banana Cultivation and Research
- Mycotoxins in Agriculture and Food
- Fungal Plant Pathogen Control
- Plant Genetic and Mutation Studies
- Plant Micronutrient Interactions and Effects
- Food Quality and Safety Studies
Nanjing Agricultural University
2016-2025
Calcium-dependent protein kinases (CDPKs) are important calcium receptors, which play a crucial part in the process of sensing and decoding intracellular signals during plant development adaptation to various environmental stresses. In this study, CDPK gene MdCPK1a , was isolated from apple ( Malus×domestica ) contains 1701bp nucleotide encodes 566 amino acid residues, conserved domain CDPKs. The transient expression western blot experiment showed that localized nucleus cell plasma membrane....
Abstract The anthocyanin content in apple skin determines its red coloration, as seen a Fuji mutant. Comparative RNA-seq analysis was performed to determine differentially expressed genes at different fruit development stages between the wild-type and color A novel R2R3-MYB transcription factor, MdMYB90-like, uncovered key regulatory gene for enhanced coloration expression of MdMYB90-like 21.3 times higher regulates biosynthesis directly through activation indirectly other factors that...
Abstract The Alternaria alternata apple pathotype adversely affects ( Malus domestica Borkh.) cultivation. However, the molecular mechanisms underlying enhanced resistance to this pathogen in remain poorly understood. We have previously reported that MdWRKY75 expression is upregulated by A. infection ‘Sushuai’ apples. In study, we discovered overexpression of MdWRKY75e increased transgenic lines infection, whereas silencing gene susceptibility infection. Furthermore, found directly binds...
Powdery mildew (PM), caused by the biotrophic fungus Podospharea leucotricha, poses a significant threat to apple production. Salicylic acid (SA) signaling plays crucial role in enhancing resistance pathogens. While PR1, defense protein induced SA, is essential for plant immunity, its excessive accumulation can be detrimental. However, mechanism of PR1-mediated immune balance remains unclear. This study identified key transcription factor, WRKY1, which enhances SA modulating biosynthesis...
Many studies have been done to find out the molecular mechanism of systemic acquired resistance (SAR) in plants past several decades. Numbers researches carried model such as arabidopsis, tobacco, rice and so on, however, with little work woody especially fruit trees apple. Components pathway SAR seem be extremely conserved variety species. Malus hupehensis, which is origin China, strong rootstock. In study, we attempted make expression pattern pathogenesis related (PR) genes were downstream...
Abstract Most commercially grown apple cultivars are susceptible to fungal diseases. Malus hupehensis has high resistance many diseases affecting cultivars. Understanding innate defence mechanisms would help develop disease‐resistant crops. Non‐expressor of pathogenesis‐related genes 1 ( NPR 1) plays a key role in regulating salicylic acid (SA)‐mediated systemic acquired (SAR). MhNPR1 cDNA, corresponding genomic DNA and its 5′ flanking sequences, was isolated from M. . Sequence analysis...
Early ripening in grape (Vitis vinifera L.) is a crucial agronomic trait. The fruits of the line 'Summer Black' (SBBM), which contains bud mutation, can be harvested approximately one week earlier than (SBC)control. To investigate molecular mechanism mutation related to early ripening, we detected genome-wide genetic variations based on re-sequencing. In total, 3,692,777 single nucleotide polymorphisms (SNPs) and 81,223 structure (SVs) SBC genome 3,823,464 SNPs 85,801 SVs SBBM were compared...
MicroRNA (miRNA)-mediated post-transcriptional regulation plays a fundamental role in various plant physiological processes, including responses to pathogens. MicroRNA168 has been implicated as an essential factor of miRNA pathways by targeting ARGONAUTE1 (AGO1), the core component RNA-induced silencing complex (RISC). A fluctuation AGO1 expression influences plant–pathogen interactions, and homeostasis miR168 accumulation is maintained complicated feedback regulatory loop. In this study,...
The apple (Malus × domestica Borkh.) cultivar 'Su Shuai' exhibits greater disease resistance, shorter internodes and lighter fruit flavor compared with its parents 'Golden Delicious' 'Indo'. To obtain a comprehensive overview of the sequence variation in these three horticultural traits, genomes 'Indo' were resequenced using next-generation sequencing to genome Delicious'. A wide range genetic variations detected, including 2 454 406 18 749 349 single nucleotide polymorphism (SNP) 59 547 50...
Growth monitoring indicated that the height of 'Kanshu' plants with 'Nantong-xiaofangshi' as an interstock was significantly shorter than no interstock. A transcriptome analysis two graft combinations ('Kanshu'/Diospyros lotus and 'Kanshu'/'Nantong-xiaofangshi'/Diospyros lotus) conducted to explore dwarfing genes related use Hormone levels water conductance were also measured in these combinations. The results both IAA GA lower had been grafted onto interstock; additionally, grafts...
Abstract The formation and regulation of loquat fruit quality have always been an important research field to improve quality, commodities, market value. Fruit size, soluble solids content, titratable acid content represent the most factors in loquat. Mineral nutrients abundance or deficiency are among key factor that affect quality. In present study, we use artificial neural network (ANN) explore effects mineral soil leaves on results show ANN model with structure 12–12–1 can predict single...
Powdery mildew is one of the most serious diseases in apple production. SAR has a broad-spectrum immunity plants against pathogen. Plants activate pathogen invasion and also prevent over-immunity. The relevant mechanism still unknown apple. In this study, we isolated identified powdery from field preserved them on tissue culture seedlings. We performed DAP-seq mildew-inducible WRKY40. WRKY40 positively regulates NPR3like by directly binding to W-box element its promoter. represses expression...
Soluble sugar and organic acids are key determinants of fruit organoleptic quality directly affect the commodity value economic returns crops. We performed whole-genome sequencing apple varieties Gala Xiahongrou, along with their F1 hybrids, to construct a high-density bin map. Our quantitative genetic analysis pinpointed 53 trait loci (QTLs) related 11 acid traits. identified candidate gene, MdNADP-ME, responsible for malate degradation, in stable QTL on linkage group 15. Sequence revealed...
Abstract Background Apple is one of the most popular fruit crops world-wide and its skin color an important quality consideration essential for commercial value. However, strategy on genetic breeding red apple basis differentiation very limited still largely unknown. Results Here, we reported a bud sport mutant Fuji with enhanced anthocyanins accumulation. Quantitative SWATH-MS (sequential window acquisition all theoretical spectra-mass spectrometry) proteomics investigations revealed...
Abstract MicroRNA (miRNA)-mediated gene silencing is a master regulatory pathway in plant–pathogen interactions. The differential accumulation of miRNAs among plant varieties alters the expression target genes, affecting defense responses and causing resistance differences varieties. Botryosphaeria dothidea an important phytopathogenic fungus apple (Malus domestica). Malus hupehensis (Pamp.) Rehder, wild species, highly resistant, whereas cultivar “Fuji” susceptible. Here, we identified...
The success of quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) to quantify gene expression depends on the stability reference genes used for data normalization. To date, systematic screening in persimmon (Diospyros kaki Thunb) has never been reported. In this study, 13 candidate were cloned from 'Nantongxiaofangshi' using information available transcriptome database. Their was assessed by geNorm and NormFinder algorithms under abiotic stress hormone...