A5084350382- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Plant Molecular Biology Research
- Postharvest Quality and Shelf Life Management
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- Horticultural and Viticultural Research
- Plant nutrient uptake and metabolism
- Biochemical and biochemical processes
- Ecology and Conservation Studies
- Agriculture, Soil, Plant Science
- Molecular Biology Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Greenhouse Technology and Climate Control
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
Gansu Agricultural University
2022-2024
Nanjing Agricultural University
2014-2022
Abstract The Alternaria alternata apple pathotype adversely affects ( Malus domestica Borkh.) cultivation. However, the molecular mechanisms underlying enhanced resistance to this pathogen in remain poorly understood. We have previously reported that MdWRKY75 expression is upregulated by A. infection ‘Sushuai’ apples. In study, we discovered overexpression of MdWRKY75e increased transgenic lines infection, whereas silencing gene susceptibility infection. Furthermore, found directly binds...
MicroRNA (miRNA)-mediated post-transcriptional regulation plays a fundamental role in various plant physiological processes, including responses to pathogens. MicroRNA168 has been implicated as an essential factor of miRNA pathways by targeting ARGONAUTE1 (AGO1), the core component RNA-induced silencing complex (RISC). A fluctuation AGO1 expression influences plant–pathogen interactions, and homeostasis miR168 accumulation is maintained complicated feedback regulatory loop. In this study,...
The apple (Malus × domestica Borkh.) cultivar 'Su Shuai' exhibits greater disease resistance, shorter internodes and lighter fruit flavor compared with its parents 'Golden Delicious' 'Indo'. To obtain a comprehensive overview of the sequence variation in these three horticultural traits, genomes 'Indo' were resequenced using next-generation sequencing to genome Delicious'. A wide range genetic variations detected, including 2 454 406 18 749 349 single nucleotide polymorphism (SNP) 59 547 50...
Abstract MicroRNA (miRNA)-mediated gene silencing is a master regulatory pathway in plant–pathogen interactions. The differential accumulation of miRNAs among plant varieties alters the expression target genes, affecting defense responses and causing resistance differences varieties. Botryosphaeria dothidea an important phytopathogenic fungus apple (Malus domestica). Malus hupehensis (Pamp.) Rehder, wild species, highly resistant, whereas cultivar “Fuji” susceptible. Here, we identified...
The success of quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) to quantify gene expression depends on the stability reference genes used for data normalization. To date, systematic screening in persimmon (Diospyros kaki Thunb) has never been reported. In this study, 13 candidate were cloned from 'Nantongxiaofangshi' using information available transcriptome database. Their was assessed by geNorm and NormFinder algorithms under abiotic stress hormone...
'Sushuai' is a disease-resistant apple variety. Transcriptome sequencing revealed differentially expressed genes mainly in the metabolism, biosynthesis of secondary metabolites, plant–pathogen interactions, and plant signaling transduction pathways. The jasmonate/ethylene pathway WRKY transcription factors (especially WRKY75 genes) played important roles improving Alternaria alternata resistance 'Sushuai'. An investigation effect biotic abiotic stresses on expression MdWRKY75s that MdWRKY75d...
Sucrose phosphate synthase (SPS) is an important link in the process of sugar metabolism. In addition, it also involved abiotic stresses plants. order to study SPS gene family and its role stress, we identified MdSPS members by bioinformatics methods such as correlation analysis, HMM method, Clustering analyzed transient expression genes quantitative real-time fluorescence analysis (qRT-PCR). The consists a total 19 divided into three subfamilies distributed on 14 chromosomes apples. has 12...