Familial Parkinson disease is associated with mutations in α-synuclein (α-syn), a presynaptic protein that has been localized not only to the cytosol, but also mitochondria. We report here wild-type α-syn from cell lines, and brain tissue humans mice, present mitochondria rather mitochondria-associated endoplasmic reticulum (ER) membranes (MAM), structurally functionally distinct subdomain of ER. Remarkably, we found pathogenic point human result its reduced association MAM, coincident lower...

In potato (Solanum tuberosum L.), R2R3 MYBs are involved in the regulation of anthocyanin biosynthesis. We examined sequences these cultivated potatoes, which more complex than diploid due to ploidy and heterozygosity. found amino acid variants C-terminus MYB StAN1, termed R0, R1, R3, presence a repeated 10-amino motif. These variant showed some expression both white pigmented tubers. several new alleles or gene family members MYBs, StMYBA1 StMYB113, were also expressed From functional...

The woodland strawberry, Fragaria vesca is a model fruit for number of rosaceous crops. We have engineered altered concentrations anthocyanin in F. vesca, to determine the impact on plant growth and quality. Anthocyanin were significantly increased by over-expression or decreased knock-down R2R3 MYB activator, MYB10. In contrast, potential bHLH partner MYB10 (bHLH33) did not affect pathway when knocked down using RNAi constructs. Metabolic analysis fruits revealed that, all polyphenolics...

High temperatures are known to reduce anthocyanin accumulation in a number of diverse plant species. In potato (Solanum tuberosum L.), high temperature significantly reduces tuber pigment content. However, the mechanism biosynthesis under heat stress remains unknown. Here we show that causes reduction both skin and flesh, with white areas forming between vasculature periderm. Heat reduced expression R2R3 MYB transcription factors (TFs) StAN1 StbHLH1, members transcriptional complex...

Introduction The potato (Solanum tuberosum) cultivar 'Xin Daping' is tetraploid with white skin and flesh, while the 'Hei Meiren' also purple flesh. Comparative transcriptome analysis of cultivars was carried out using high-throughput RNA sequencing in order to further understand mechanism anthocyanin biosynthesis potato. Methods Results By aligning transcript reads recently published diploid genome de novo assembly, 209 million paired-end Illumina RNA-seq from these were assembled on 60,930...

Knowledge about fungi diversity following different planting patterns could improve our understanding of soil processes and thus help us to develop sustainable management strategies. The objective this study was determine the impact furrow-ridge mulching techniques on fungal in rhizosphere under continuous cropping system. investigated treatments were: flat plot without mulch (CK); with (T1); on-ridge full (T2); on-furrow (T3); half (T4); (T5). NGS (Illumina) methods ITS1 sequences were used...

This study was conducted to explore the changes in soil microbial populations, enzyme activity, and tuber yield under rotation sequences of Potato–Common vetch (P–C), Potato–Black medic (P–B) Potato–Longdong alfalfa (P–L) a semi–arid area China. The also determined effects continuous potato cropping (without legumes) on above mentioned properties yield. number bacteria increased significantly (p < 0.05) P–B by 78%, 85% 83% 2, 4 7–year soils, respectively compared P–C rotation. highest...

Maintaining a pool of functional mitochondria requires degradation damaged ones within the cell. PINK1 is critical in this quality-control process: loss mitochondrial membrane potential causes to accumulate on surface, triggering mitophagy. However, little known about how regulated. Recently, we showed that content kept low healthy by continuous ubiquitination and proteasomal its mature form via mechanism inconsistent with proposed N-end rule process. Using both human female monkey cell...

Abstract Background Cadmium (Cd) pollution has brought harm to the growth and development of potato. Glutathione (GSH) is an important antioxidant that may play active role in response a potato Cd stress. However, how GSH influences effect on potatoes unknown. In this study, we investigated effects exogenous phenylpropanoid biosynthesis pathway plant hormone signal transduction under stress explore new ideas for respond We cultured 21-day-old 'Atlantic’ plantlets Murashige Skoog (MS) medium...

Along with Parkin, PINK1 plays a critical role in maintaining mitochondrial quality control. Although is expressed constitutively, its level kept low healthy mitochondria by polyubiquitination and ensuing proteasomal degradation of mature, 52 kDa, form. We show here that the target mature form mediated ubiquitination conserved lysine at position 137. Notably, full-length protein also contains Lys-137 but not ubiquitinated. On basis our data, we propose cleavage Phe-104 disrupts major...

Survival of plants in response to salinity stress is typically related Na+ toxicity, but little known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages potato (Solanum tuberosum L.). In this study, we used the Arabidopsis thaliana gene (AtHKT1) enhance capacity tolerate by decreasing content and improving K+/Na+ ratio plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed lines (namely T1, T2, T3, T5, T11, T13 T15)...

Abstract Background Continuous cropping can reduce soil quality and affect rhizosphere metabolism, ultimately reducing crop yield. Crop rotation mitigate the damage caused by continuous cropping, but different patterns respond differently to metabolism. We investigated effects of on physicochemical properties, enzyme activities, microbial quantity, metabolism potato based a long-term field study from 2018 2022. The experiment was set up with following three treatments: ( Solanum tuberosum...

Abstract Background Plant U-box (PUB) E3 ubiquitin ligases have vital effects on various biological processes. Therefore, a comprehensive and systematic identification of the members gene family in potato will help to understand evolution function plants. Results This work identified altogether 74 PUBs (StPUBs) examined their structures, chromosomal distributions, conserved motifs. There were seventy-four StPUB genes ten chromosomes with diverse densities. As revealed by phylogenetic...

The synthesis or derivation of a series structural analogues based on natural products is common strategy for discovering antitumor drugs. As unique diterpene derived from Trigonostemon howii, 3,4-seco-sonderianol (1) exhibited moderate cytotoxic effects. To improve the activity, new derivative (1b) with mitochondrial targeting function was synthesized by coupling triphenylphosphine to compound 1. Compared parent molecule, activity 1b increased greatly. A mechanistic experiments revealed...

Mutations in the MERTK gene are responsible for retinal degeneration Royal College of Surgeons (RCS) rat and a cause human autosomal recessive retinitis pigmentosa (RP). This study reports identification functional analysis novel mutations to provide information regarding whether they causative severe rod-cone young patient.MERTK missense variants identified by single-strand conformational polymorphism (SSCP) sequence were introduced into expression constructs used transfect HEK293T cells....

Survival and mortality of plants in response to severe drought may be related carbon starvation, but little is known about how plasma membrane intrinsic proteins help alleviate the drought-induced consequence. Here, we determined roles plasmalemma aquaporin gene improving plant water status, maintaining accumulation, thereby enhancing tolerance. Seven StPIP1 transformed potato (Solanum tuberosum L.) lines (namely T1, T2…T7) were compared with non-transgenic control at molecule whole-plant...

Potatoes consist of flavonoids that provide health benefits for human consumers. To learn more about how potato tuber flavonoid accumulation and flesh pigmentation are controlled, we analyzed the transcriptomic metabolomic profile tubers from three colored clones at developmental phases using an integrated approach. From 72 identified in pigmented flesh, differential abundance was noted anthocyanins, flavonols, flavones. Weighted gene co-expression network analysis further allowed modules...

Hepatocellular carcinoma (HCC) seriously threatens human health, mostly developed from liver fibrosis or cirrhosis. Since diethylnitrosamine (DEN) and carbon tetrachloride (CCl4)-induced HCC mouse model almost recapitulates the characteristic of with inflammation, it is taken as an essential tool to investigate pathogenesis HCC. However, a comprehensive understanding protein expression profile this little. In study, we performed proteomic analysis elucidate its characteristics. Compared...

ABSTRACT Alkaline salt stress, as a more diverse severely affects the growth and development of potato ( Solanum tuberosum L.) leads to yield reduction. Brassinosteroids have been shown regulate plant play an essential role under environmental stress. However, physiological responses by which brassinosteroids confer alkaline stress tolerance in remain unclear. We used ‘ Atlantic ’ experimental material. The effects 0.01, 0.1, 1 10 μmol·L −1 24‐epibrassinolide (EBR) on photosynthetic...