A5054500913- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Mycorrhizal Fungi and Plant Interactions
- Traditional Chinese Medicine Analysis
- Genetic diversity and population structure
- Fungal Biology and Applications
- Nephrotoxicity and Medicinal Plants
- Plant and Fungal Species Descriptions
- Medicinal Plant Pharmacodynamics Research
- Natural product bioactivities and synthesis
- Ginseng Biological Effects and Applications
- Phytochemistry and Biological Activities
- Traditional Chinese Medicine Studies
- Plant and animal studies
- Photosynthetic Processes and Mechanisms
- Ethnobotanical and Medicinal Plants Studies
- Plant-based Medicinal Research
- Ginger and Zingiberaceae research
- Gut microbiota and health
- Cancer-related molecular mechanisms research
- Glycosylation and Glycoproteins Research
- MicroRNA in disease regulation
- Environmental DNA in Biodiversity Studies
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
South China University of Technology
2024
Ministry of Education of the People's Republic of China
2020-2022
State Administration of Traditional Chinese Medicine of the People's Republic of China
2018-2021
Zhengzhou University
2017
Henan Provincial People's Hospital
2017
Sichuan University
2012-2014
State Key Laboratory of Biotherapy
2013
West China Hospital of Sichuan University
2013
Shanghai Jiao Tong University
2010
The plant working group of the Consortium for Barcode Life recommended two-locus combination rbcL+matK as barcode, yet was shown to successfully discriminate among 907 samples from 550 species at level with a probability 72%. admits that barcode is far perfect due low identification rate, and search not over.Here, we compared seven candidate DNA barcodes (psbA-trnH, matK, rbcL, rpoC1, ycf5, ITS2, ITS) medicinal species. Our ranking criteria included PCR amplification efficiency, differential...
Background The internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA is regarded as one the candidate barcodes because it possesses a number valuable characteristics, such availability conserved regions for designing universal primers, ease its amplification, and sufficient variability to distinguish even closely related species. However, general analysis ability discriminate species in comprehensive sample set lacking. Methodology/Principal Findings In current study, 50,790...
The presence of duplicates introduced by PCR amplification is a major issue in paired short reads from next-generation sequencing platforms. These might have serious impact on research applications, such as scaffolding whole-genome and discovering large-scale genome variations, are usually removed. We present FastUniq fast de novo tool for removal reads. identifies comparing sequences between read pairs does not require complete prerequisites. capable simultaneously handling with different...
Salvia miltiorrhiza is an important medicinal plant with great economic and value. The complete chloroplast (cp) genome sequence of miltiorrhiza, the first sequenced member Lamiaceae family, reported here. 151,328 bp in length exhibits a typical quadripartite structure large (LSC, 82,695 bp) small (SSC, 17,555 single-copy regions, separated by pair inverted repeats (IRs, 25,539 bp). It contains 114 unique genes, including 80 protein-coding 30 tRNAs four rRNAs. structure, gene order, GC...
Abstract Versatile strategies are currently being discovered for the fabrication of synthetic polypeptide‐based hybrid hydrogels, which have potential applications in polymer therapeutics and regenerative medicine. Herein, a new concept—the reverse micellar hydrogel—is introduced, versatile strategy is provided fabricating supramolecular normal hydrogel hydrogels from same copolymer via cooperation host–guest chemistry hydrogen‐bonding interactions. The thoroughly characterized, mechanism...
Abstract Adulterant herbal materials are a threat to consumer safety. In this study, we used DNA barcoding investigate the proportions and varieties of adulterant species in traditional Chinese medicine (TCM) markets. We barcode database TCM (TCMD) that was established by our group 1436 samples representing 295 medicinal from 7 primary markets China. The results indicate ITS2 barcodes could be generated for most (87.7%) using standard protocol. Of 1260 samples, approximately 4.2% were...
Background Internal transcribed spacer of nuclear ribosomal DNA (nrDNA) is already one the most popular phylogenetic and barcoding markers. However, existence its multiple copies has complicated such usage a detailed characterization intra-genomic variations critical to address concerns. Methodology/Principal Findings In this study, we used sequence-tagged pyrosequencing genome-wide analyses characterize internal 2 (ITS2) regions from 178 plant species. We discovered that mutation ITS2...
In this study, we tested the applicability of four DNA regions (rbcL, matK, ITS, and ITS2) as barcodes for identifying species within Euphorbiaceae. Based on assessments specific genetic divergence, barcoding gap, ability discrimination, present results affirmed that ITS/ITS2 is a potential barcode Euphorbiaceae species. This study also provided large-scale test to evaluate effectiveness differentiating Of 1183 plant samples collected from 871 in 66 diverse genera, successfully identified >...
In Dunaliella tertiolecta, a microalga renowned for its extraordinary tolerance to high salinity levels up 4.5 M NaCl, the mechanisms underlying stress response have largely remained mystery. groundbreaking discovery, this study identifies choline dehydrogenase enzyme, termed DtCHDH, capable of converting betaine aldehyde. Remarkably, is first identification such an enzyme not just in D. tertiolecta but across entire Chlorophyta. A 3D model DtCHDH was constructed, and molecular docking with...
Excessive utilization of chemical fertilizers degrades the quality medicinal plants and soil. Bio-organic (BOFs) including microbial inoculants microalgae have garnered considerable attention as potential substitutes for fertilizer to enhance yield. In this study, a field experiment was conducted investigate effects BOF partially substituting on growth plant Polygala tenuifolia. The parameters, bioactive component contents, soil properties composition rhizosphere microorganisms were...
American ginseng (derived from Panax quinquefolius) is one of the most widely used medicinal herbs in world. Because its high price and increasing demand, there are many adulterants on market. The proposed internal transcribed spacer 2 (ITS2) has been to identify raw materials, but it not suitable for identification Chinese patent medicine ingredients. Therefore, a short barcode processed corresponding medicines would be profitable. In this study, 94 samples Asian were collected all over...
Acanthopanacis cortex has been used in clinical applications for a long time. Considering some historical and geographical factors, is easily confused with other herbs medicine markets, thereby causing potential safety issues. In this study, we the internal transcribed spacer 2 (ITS2) barcode to identify 69 samples belonging six species, including its adulterants. The nearest distance, single-nucleotide polymorphisms (SNPs), neighbor-joining (NJ) tree methods were evaluate identification...
Epimedium L., the largest herbaceous genus of Berberidaceae, is one most taxonomically difficult representatives. The classification and phylogenetic relationships within are controversial unresolved.For first time, we systematically studied phylogeny evolution based on plastid genome (plastome) data for better understanding this enigmatic genus.We explored molecular phylogeny, assessed infrageneric classification, estimated divergence times, inferred ancestral states flower traits 45...
The DNA barcoding technology uses a standard region of sequence for species identification and discovery. At present, "DNA barcode" actually refers to sequences, which are not amenable information storage, recognition, retrieval. Our aim is identify the best symbology that can represent barcode sequences in practical applications. A comprehensive set five markers ITS2, rbcL, matK, psbA-trnH, CO1 was used as test data. Fifty-three different types one-dimensional ten two-dimensional...
DNA barcoding technology, which uses a short piece of sequence to identify species, has wide ranges applications. Until today, universal barcode marker for plants remains elusive. The rbc L and mat K regions have been proposed as the "core barcode" ITS2 psbA-trnH intergenic spacer (PTIGS) were later added supplemental barcodes. use PTIGS region limited by lack computational tools that can handle significant insertions deletions in sequences. Here, we compared most commonly used...