A5046142132- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Insect and Pesticide Research
- Mycorrhizal Fungi and Plant Interactions
- Pesticide Residue Analysis and Safety
- Medicinal Plant Pharmacodynamics Research
- Fungal Biology and Applications
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Plant and animal studies
- Natural product bioactivities and synthesis
- Clay minerals and soil interactions
- CRISPR and Genetic Engineering
- Insect and Arachnid Ecology and Behavior
- Antibiotics Pharmacokinetics and Efficacy
- Pharmacological Effects and Toxicity Studies
- Insect Pest Control Strategies
- Gut microbiota and health
- Plant and fungal interactions
- Food Quality and Safety Studies
- Healthcare and Venom Research
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- Botanical Studies and Applications
- Heavy Metals in Plants
Chinese Academy of Medical Sciences & Peking Union Medical College
2017-2025
Chinese Academy of Agricultural Sciences
2022-2024
Institute of Plant Protection
2022-2024
Peking Union Medical College Hospital
2024
Academy of Medical Sciences
2024
Ningbo University of Technology
2024
Fudan University
2024
Ministry of Education of the People's Republic of China
2020-2022
Huazhong Agricultural University
2022
State Administration of Traditional Chinese Medicine of the People's Republic of China
2018-2020
In this work, a green analytical method was established for the simultaneous extraction and detection of 20 analytes-10 neonicotinoid insecticides their 10 major toxic metabolites in edible herbs. QuEChERS LC-MS/MS were used to analyze analytes five The residues 109 herbal samples detected, which 90 positive, residue total ranged from 0.26 139.28 μg/kg. Acetamiprid (77.06 %, ≤85.95 μg/kg), imidacloprid (67.89 ≤32.49 μg/kg) (N-desmethyl-acetamiprid (44.04 ≤18.42 desnitro (48.62 ≤16.55 most...
Cytosine base editors (CBEs) show promise for multiplex gene knockout applications, but impure edits, indels and off-targets still frequently occur. We describe here QBEmax, which exhibits high efficiency, low indel product purity with up to 99.8% of edits comprised C-to-T. Through molecular dynamic modeling, QBEmax presents as a compact stable editor that shields protected bases from undesired repair processes.
Epimedium L., the largest herbaceous genus of Berberidaceae, is one most taxonomically difficult representatives. The classification and phylogenetic relationships within are controversial unresolved.For first time, we systematically studied phylogeny evolution based on plastid genome (plastome) data for better understanding this enigmatic genus.We explored molecular phylogeny, assessed infrageneric classification, estimated divergence times, inferred ancestral states flower traits 45...
Aspergillus flavus produces highly toxic and carcinogenic aflatoxins that cause severe economic losses pose a serious threat to public health. Cinnamon essential oils (CEO) have recently attracted attention as promising alternative antifungal agents. In this study, the inhibition mechanism of CEO on A. was investigated, its application in prevention coix seeds explored for first time. The results showed mycelial growth, spore germination, aflatoxin synthesis were completely inhibited after...
Cynanchum is a large genus with some important medicinal species in China. The are easily confused, leading to potential safety risks. In this study, the internal transcribed spacer 2 (ITS2) barcode was used discriminate plants Cynanchum. identifying capability of ITS2 assessed using specific genetic divergence, BLAST1, neighbor-joining (NJ) tree, maximum-likelihood (ML) and single-nucleotide polymorphism (SNP) methods. Results indicated that intra-specific divergences were lower than their...
Ziziphi Spinosae Semen (ZSS) has been widely used in traditional Chinese medicine system for decades. Under proper humidity and temperature, ZSS is easily contaminated by fungi mycotoxins during harvest, storage, transport, thereby posing a considerable threat to consumer health. In this study, we first the Illumina MiSeq PE250 platform targeted internal transcribed spacer 2 sequences investigate presence of moldy normal samples collected from five producing areas China. Results showed that...
Platycladi Semen, which is derived from the dried ripe seed of Platycladus orientalis, has been used for treatment insomnia and constipation in China 2000 years. However, it susceptible to fungal aflatoxin contamination under proper humidity temperature during storage. Although Semen reported preliminarily, few studies have conducted on infection simultaneously. Thus, this work aims provide an in-depth understanding information contamination. We focused a comparison difference diversity...
Genus Epimedium consists of approximately 50 species in China, and more than half them possess medicinal properties. The high similarity species' morphological characteristics complicates the identification accuracy, leading to potential risks herbal efficacy medical safety. In this study, we tested applicability four single loci, namely, rbcL, psbA-trnH, internal transcribed spacer (ITS), ITS2, their combinations as DNA barcodes identify 37 on basis analyses, including success rates PCR...
Maize yellow mosaic virus (MaYMV), a new species in the genus Polerovirus (family Solemoviridae), was reported maize for first time China 2016 (Chen et al., 2016). Later, MaYMV found other gramineous including sugarcane (Saccharum spp.), itch grass (Rottboellia cochinchinensis), millet (Panicum miliaceum) and sorghum (Sorghum bicolor) several countries Asia, Africa, South America (Yahaya al. 2017; Lim 2018; Sun 2019; Nithya 2021). Here, we report its presence cultivated wheat (Triticum...
Morindae Officinalis Radix (MOR) and Alpiniae Oxyphyllae Fructus (AOF) have been widely used as dietary supplements traditional herbal medicines for centuries. Fungal mycotoxin contamination in MOR AOF has reported recently. In this study, fungi are first investigated using DNA metabarcoding, the differences fungal microbiome between moldy non-moldy samples analyzed. The results show that Ascomycota is most prevailing fungus at phylum level with relative abundances of 49.53-94.32%...
Schisandrae Chinensis Fructus (Wuweizi) is often adulterated with Sphenantherae (Nanwuweizi) in the herbal market. This adulteration a threat to clinical treatment and safety. In this study, we aimed develop nucleotide signature for identification of Wuweizi its Chinese patent medicines based on mini-DNA barcoding technique. We collected 49 samples obtain internal transcribed spacer 2 (ITS2) sequences developed 26-bp (5′-CGCTTTGCGACGCTCCCCTCCCTCCC-3′) basis single polymorphism (SNP) site...