A5002876293- Genetically Modified Organisms Research
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Cocoa and Sweet Potato Agronomy
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Botany and Plant Ecology Studies
- Genetic diversity and population structure
- Plant Disease Resistance and Genetics
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Atmospheric chemistry and aerosols
- Plant responses to elevated CO2
- Transgenic Plants and Applications
- Identification and Quantification in Food
- Plant and animal studies
- Plant Taxonomy and Phylogenetics
- Plant responses to water stress
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Food Chemistry and Fat Analysis
- Potato Plant Research
Aberystwyth University
2011-2025
Institute of Biological, Environmental and Rural Sciences
2009-2022
Lancaster University
2006-2021
University of California, San Diego
2021
University of Reading
2001-2017
Google (United States)
2016
The University of Adelaide
2012-2015
Wine Australia
2015
Edward Hospital
2013
University of Colorado Boulder
2007-2012
DNA barcoding involves sequencing a standard region of as tool for species identification. However, there has been no agreement on which region(s) should be used land plants. To provide community recommendation plant barcode, we have compared the performance 7 leading candidate plastid regions (atpF-atpH spacer, matK gene, rbcL rpoB rpoC1 psbK-psbI and trnH-psbA spacer). Based assessments recoverability, sequence quality, levels discrimination, recommend 2-locus combination rbcL+matK...
Abstract We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. review the other markers that have been proposed and discuss their advantages disadvantages. The low levels variation make necessary; there are no regions, coding or non‐coding, evolve rapidly mitochondrial generally does animals. outline two, three‐region options, (1) rpoC1, rpoB 1matK (2) matK psbA‐trnH viable plant barcoding.
An in silico screen of 41 the 81 coding regions Nicotiana plastid genome generated a shortlist 12 candidates as DNA barcoding loci for land plants. These were evaluated amplification and sequence variation against reference set 98 plant taxa. The deployment multiple primers modified multiplexed tandem polymerase chain reaction yielded 85–94% across taxa, mean differences between sister taxa 6.1 from 156 bases accD to 22 493 matK. We conclude that should be combined effective diagnosis,...
We present the first national DNA barcode resource that covers native flowering plants and conifers for nation of Wales (1143 species). Using plant markers rbcL matK, we have assembled 97.7% coverage rbcL, 90.2% a dual-locus 89.7% Welsh flora. sampled multiple individuals each species, resulting in 3304 2419 matK sequences. The majority our samples (85%) are from extracted herbarium specimens. Recoverability barcodes is lower using specimens, compared to freshly collected material, mostly...
Abstract We explore the potential role of atmospheric carbon dioxide (CO 2 ) on isoprene emissions using a global coupled land–atmosphere model [Community Atmospheric Model–Community Land Model (CAM–CLM)] for recent (year 2000, 365 ppm CO and future 2100, 717 conditions. incorporate an empirical observed response to both ambient concentrations in long‐term growth environment short‐term changes intercellular into MEGAN biogenic emission embedded within CLM. Accounting inhibition has little...
Several fluorescent probes were evaluated as indicators of bacterial viability by flow cytometry. The monitor a number biological factors that are altered during loss viability. include alterations in membrane permeability, monitored using fluorogenic substrates and intercalating dyes such propidium iodide, changes potential, cationic anionic potential-sensitive probes. Of the reagents examined, potential probe bis-(1,3-dibutylbarbituric acid)trimethine oxonol [DiBAC(inf4)(3)] proved best...
Abstract There is considerable interest in modeling isoprene emissions from terrestrial vegetation, because these exert a principal control over the oxidative capacity of troposphere. We used unique field experiment that employs continuous gradient CO 2 concentration 240 to 520 ppmv demonstrate Eucalyptus globulus were enhanced at lowest concentration, which was similar estimated concentrations during last Glacial Maximum, compared with 380 ppmv, current concentration. Leaves Liquidambar...
Background Internal transcribed spacer of nuclear ribosomal DNA (nrDNA) is already one the most popular phylogenetic and barcoding markers. However, existence its multiple copies has complicated such usage a detailed characterization intra-genomic variations critical to address concerns. Methodology/Principal Findings In this study, we used sequence-tagged pyrosequencing genome-wide analyses characterize internal 2 (ITS2) regions from 178 plant species. We discovered that mutation ITS2...
Environmental cues influence the development of stomata on leaf epidermis, and allow plants to exert plasticity in stomatal abundance response prevailing growing conditions. It is reported that Arabidopsis thaliana 'Landsberg erecta' grown under low relative humidity have a reduced index two genes pathway, SPEECHLESS FAMA, become de novo cytosine methylated transcriptionally repressed. These environmentally-induced epigenetic responses were abolished mutants lacking capacity for DNA...
Similar to other metazoan pathogens, Schistosoma mansoni undergoes transcriptional and developmental regulation during its complex lifecycle host interactions. DNA methylation as a mechanism control these processes has, date, been discounted in this parasite. Here we show the first evidence for cytosine S. genome. Transcriptional coregulation of novel methyltransferase (SmDnmt2) methyl-CpG-binding domain proteins mirrors detection abundance implicates presence functional machinery. Genome...
Measures blocking hybridization would prevent or reduce biotic environmental change caused by gene flow from genetically modified (GM) crops to wild relatives. The efficacy of any such measure depends on hybrid numbers within the legislative region over life-span GM cultivar. We present a national assessment between rapeseed ( Brassica napus ) and B. rapa combination sources, including population surveys, remote sensing, pollen dispersal profiles, herbarium data, local Floras, other...
Coupled surface–atmosphere models are being used with increased frequency to make predictions of tropospheric chemistry on a ‘future’ earth characterized by warmer climate and elevated atmospheric CO 2 concentration. One the key inputs these is emission isoprene from forest ecosystems. Most in current use rely scheme which global change coupled changes terrestrial net primary productivity (NPP) which, turn, magnitude emissions. In this study, we conducted measurements emissions at three...
• Relatively little is known about the timing of genetic and epigenetic forms somaclonal variation arising from callus growth. We surveyed for both types change in cocoa (Theobroma cacao) plants regenerated calli various ages, also between tissues source trees. For change, we used 15 single sequence repeat (SSR) markers four trees 233 plants. 386 methylation-sensitive amplified polymorphism (MSAP) on leaf explant (staminode) DNA two 114 regenerants. Genetic within was limited to one slippage...
Abstract One of the major environmental concerns over genetically modified (GM) crops relates to transgene movement into wild relatives. The pattern hybridization ultimately affects scale and rapidity ecological change feasibility containment. A new procedure for quantifying hybrid formation large areas is described. Remote sensing was used identify possible sites sympatry between Brassica napus its progenitor species across 15 000 km 2 south‐east England in 1998. Two sympatric populations...