A5040614063- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Pathogenic Bacteria Studies
- Neurobiology and Insect Physiology Research
- Echinoderm biology and ecology
- Advanced Proteomics Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Resistance
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- Aquatic life and conservation
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Genomics, phytochemicals, and oxidative stress
- Marine Sponges and Natural Products
- Neuroendocrine regulation and behavior
- Studies on Chitinases and Chitosanases
- Mass Spectrometry Techniques and Applications
- Crustacean biology and ecology
- Cellular transport and secretion
- Neuropeptides and Animal Physiology
RMIT University
2025
University of Warwick
2014-2023
McMaster University
1990-2023
Impact
2023
Sainsbury Laboratory
2008-2021
University of East Anglia
2020-2021
Norwich Research Park
2008-2021
Massachusetts General Hospital
2016-2019
Memorial Sloan Kettering Cancer Center
1991-2018
Cardiff University
2018
The genome of Phytophthora infestans, the pathogen that triggered Irish potato famine in nineteenth century, has been sequenced. It remains a devastating pathogen, with late blight destroying crops worth billions dollars each year. Blight is difficult to control, part because it adapts so quickly genetically resistant strains. Comparison two other genomes shows rapid turnover and extensive expansion specific families secreted disease effector proteins, including many genes induced during...
In pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), plant cell surface receptors sense potential microbial pathogens by recognizing elicitors called PAMPs. Although diverse PAMPs trigger PTI through distinct receptors, the resulting intracellular responses overlap extensively. Despite this, a common component(s) linking signal perception with transduction remains unknown. this study, we identify SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK)3/brassinosteroid-associated...
Recognition of pathogen-associated molecular patterns (PAMPs) by surface-localized pattern recognition receptors (PRRs) constitutes an important layer innate immunity in plants. The leucine-rich repeat (LRR) receptor kinases EF-TU RECEPTOR (EFR) and FLAGELLIN SENSING2 (FLS2) are the PRRs for peptide PAMPs elf18 flg22, which derived from bacterial EF-Tu flagellin, respectively. Using coimmunoprecipitation mass spectrometry analyses, we demonstrated that EFR FLS2 undergo ligand-induced...
Plants rely heavily on receptor-like kinases (RLKs) for perception and integration of external internal stimuli. The Arabidopsis regulatory leucine-rich repeat RLK (LRR-RLK) BAK1 is involved in steroid hormone responses, innate immunity, cell death control. Here, we describe the differential regulation three different BAK1-dependent signaling pathways by a novel allele BAK1, bak1-5. Innate immune mediated RKs FLS2 EFR severely compromised bak1-5 mutant plants. However, mutants are not...
Summary Advances in proteomic techniques have allowed the large‐scale identification of phosphorylation sites complex protein samples, but new biological insight requires an understanding their vivo dynamics. Here, we demonstrate use a stable isotope‐based quantitative approach for pathway discovery and structure–function studies Arabidopsis cells treated with bacterial elicitor flagellin. The comparison identifies individual on plasma membrane (PM) proteins that undergo rapid or...
Vernalization, the acceleration of flowering by winter, involves cold-induced epigenetic silencing Arabidopsis FLC. This process has been shown to require conserved Polycomb Repressive Complex 2 (PRC2) components including Su(z)12 homologue, VRN2, and two plant homeodomain (PHD) finger proteins, VRN5 VIN3. However, sequence events leading FLC repression was unclear. Here we show that, contrary expectations, VRN2 associates throughout locus independently cold. The vernalization-induced is...
Plants detect potential pathogens by sensing microbe-associated molecular patterns via pattern recognition receptors. In the dicot model plant Arabidopsis, lysin motif (LysM)-containing chitin elicitor receptor kinase 1 (CERK1) has been shown to be essential for perception of fungal cell wall component and resistance pathogens. Recent in vitro studies with CERK1 protein expressed heterologously yeast suggested direct binding activity. Here we show an affinity purification approach that is a...
The PhosPhAt database of Arabidopsis phosphorylation sites was initially launched in August 2007. Since then, along with 10-fold increase entries, functionality (phosphat.mpimp-golm.mpg.de) has been considerably upgraded and re-designed. is now more a web application the inclusion advanced search functions allowing combinatorial searches by Boolean terms. results output includes interactive visualization annotated fragmentation spectra ability to export peptide sequences as text files for...
Abstract Nitric oxide (NO) is a free radical product of cell metabolism that plays diverse and important roles in the regulation cellular function. S-Nitrosylation emerging as specific fundamental posttranslational protein modification for transduction NO bioactivity, but very little known about its physiological functions plants. We investigated molecular mechanism S-nitrosylation peroxiredoxin II E (PrxII E) from Arabidopsis thaliana found this inhibits hydroperoxide-reducing peroxidase...
The plant immune system is activated by microbial patterns that are detected as nonself molecules. Such recognized receptors cytoplasmic or localized at the plasma membrane. Cell surface represented receptor-like kinases (RLKs) frequently contain extracellular leucine-rich repeats and an intracellular kinase domain for activation of downstream signaling, well proteins (RLPs) lack this signaling domain. It therefore hypothesized RLKs required RLPs to activate signaling. Cf-4 Ve1 tomato (...
In response to pathogen attack, plant cells secrete antimicrobial molecules at the site of infection. However, how pathogens interfere with defense-related focal secretion remains poorly known. Here we show that host-translocated RXLR-type effector protein AVRblb2 Irish potato famine Phytophthora infestans focally accumulates around haustoria, specialized infection structures form inside cells, and promotes virulence by interfering execution host defenses. significantly enhances...
Accelerated gene evolution is a hallmark of pathogen adaptation following host jump. Here, we describe the biochemical basis and specialization plant effector after its colonization new host. Orthologous protease inhibitor effectors from Irish potato famine pathogen, Phytophthora infestans, sister species, mirabilis, which responsible for infection Mirabilis jalapa, are adapted to targets unique their respective plants. Amino acid polymorphisms in both inhibitors target proteases underpin...
Many eukaryotic cells use RNA-directed silencing mechanisms to protect against viruses and transposons suppress endogenous gene expression at the posttranscriptional level. RNA also is implicated in epigenetic affecting chromosome structure transcriptional silencing. Here, we describe enhanced phenotype (esp) mutants Arabidopsis thaliana that reveal how proteins associated with processing 3' end formation can influence These were a putative DEAH helicase homologue of yeast PRP2 splicing...
Abstract Alterations in the proteome of Arabidopsis (Arabidopsis thaliana) leaves during responses to challenge by Pseudomonas syringae pv tomato DC3000 were analyzed using two-dimensional gel electrophoresis. Protein changes characteristic establishment disease, basal resistance, and resistance-gene-mediated resistance examined comparing DC3000, a hrp mutant, expressing avrRpm1, respectively. The abundance each protein identified was compared with that selected transcripts obtained from...
The innate immune system allows plants to respond potential pathogens in an appropriate manner while minimizing damage and energy costs. Photosynthesis provides a sustained supply and, therefore, has be integrated into the defense against pathogens. Although changes photosynthetic activity during infection have been described, detailed conclusive characterization is lacking. Here, we addressed whether activation of early responses by pathogen-associated molecular patterns (PAMPs) triggers...
Protein conjugation with ubiquitin, known as ubiquitination, is a key regulatory mechanism to control protein abundance, localization, and activity in eukaryotic cells. To identify ubiquitin-dependent steps plants, we developed robust affinity purification/identification system for ubiquitinated proteins. Using GST-tagged ubiquitin binding domains, performed large scale purification of proteins from Arabidopsis cell suspension culture. High molecular weight were separated by SDS-PAGE, the...
BackgroundEpidemiological studies suggest that people who consume more than one portion of cruciferous vegetables per week are at lower risk both the incidence prostate cancer and developing aggressive but there is little understanding underlying mechanisms. In this study, we quantify interpret changes in global gene expression patterns human gland before, during after a 12 month broccoli-rich diet.Methods FindingsVolunteers were randomly assigned to either or pea-rich diet. After six months...
The downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa) is a filamentous oomycete that invades plant cells via sophisticated but poorly understood structures called haustoria. Haustoria are separated from the host cell cytoplasm and surrounded by an extrahaustorial membrane (EHM) of unknown origin. In some interactions, including Hpa-Arabidopsis, haustoria progressively encased host-derived, callose-rich materials molecular mechanisms which callose accumulates around remain unclear....
Plant pathogenic bacteria secrete effector proteins that attack the host signaling machinery to suppress immunity. Effectors can be recognized by hosts leading One such is AvrPtoB of Pseudomonas syringae, which degrades protein kinases, as tomato Fen, through an E3 ligase domain. Pto kinase, highly related recognizes in conjunction with resistance Prf. Here we show resistant AvrPtoB-mediated degradation because it inactivates ubiquitinated Fen within catalytic cleft, its breakdown and loss...
Abstract Bacteroidetes are abundant pathogen-suppressing members of the plant microbiome that contribute prominently to rhizosphere phosphorus mobilisation, a frequent growth-limiting nutrient in this niche. However, genetic traits underpinning their success niche remain largely unknown, particularly regarding acquisition strategies. By combining cultivation, multi-layered omics and biochemical analyses we first discovered all plant-associated express constitutive phosphatase activity,...
The cell's endomembranes comprise an intricate, highly dynamic and well-organized system. In plants, the proteins that regulate function of various endomembrane compartments their cargo remain largely unknown. Our aim was to dissect subcellular trafficking routes by enriching for partially overlapping subpopulations endosomal proteomes associated with markers. We selected RABD2a/ARA5, RABF2b/ARA7, RABF1/ARA6, RABG3f as markers combinations Golgi, trans-Golgi network (TGN), early endosomes...
Recent evidence suggests that the ubiquitin-proteasome system is involved in several aspects of plant immunity and a range pathogens subvert to enhance their virulence. Here, we show proteasome activity strongly induced during basal defense Arabidopsis (Arabidopsis thaliana). Mutant lines subunits RPT2a RPN12a support increased bacterial growth virulent Pseudomonas syringae pv tomato DC3000 (Pst) maculicola ES4326. Both are required for pathogen-associated molecular pattern-triggered...
Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically associating with molecules modulating their activities. This study aimed to identify the processes targeted RXLR class of host-translocated potato blight pathogen Phytophthora infestans. To this end, we performed an in planta protein-protein interaction screen transiently expressing P. infestans Nicotiana benthamiana leaves followed coimmunoprecipitation liquid...
Secondary metabolites are important in plant defence against pests and diseases. Similarly, insects can use secondary and, some cases, synthesize their own products. The paper describes how two specialist brassica feeders, Brevicoryne brassicae (cabbage aphid) Lipaphis erysimi (turnip sequester glucosinolates (thioglucosides) from host plants, yet avoid the generation of toxic degradation products by compartmentalizing myrosinase (thioglucosidase) into crystalline microbodies. We propose...