A5088210859- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Advanced Cellulose Research Studies
- Microbial Metabolites in Food Biotechnology
- Plant-Microbe Interactions and Immunity
- Studies on Chitinases and Chitosanases
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Food composition and properties
- Plant Molecular Biology Research
- Polysaccharides Composition and Applications
- Plant biochemistry and biosynthesis
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Aquaculture disease management and microbiota
- Plant Gene Expression Analysis
- Phytochemicals and Antioxidant Activities
- Legume Nitrogen Fixing Symbiosis
- Seaweed-derived Bioactive Compounds
- Plant Disease Resistance and Genetics
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- Microbial Community Ecology and Physiology
KTH Royal Institute of Technology
2016-2025
AlbaNova
2016-2025
The University of Adelaide
2016-2025
Flinders University
2022-2025
Agriculture and Food
2025
Australian Wine Research Institute
2024
Australian Research Council
2015-2023
ARC Centre of Excellence in Plant Energy Biology
2015-2022
Genetech (Sri Lanka)
2020
Technologies pour la Santé
2018
The genome of Phytophthora infestans, the pathogen that triggered Irish potato famine in nineteenth century, has been sequenced. It remains a devastating pathogen, with late blight destroying crops worth billions dollars each year. Blight is difficult to control, part because it adapts so quickly genetically resistant strains. Comparison two other genomes shows rapid turnover and extensive expansion specific families secreted disease effector proteins, including many genes induced during...
The authors have informed us that, in the above paper, several polypeptide GalNAc transferases were listed as part of family 2, i.e. a inverting enzymes.However, these enzymes transfer from UDP-GalNAc to Ser or Thr residues forming an α linkage.They are therefore retaining and, on this basis, they should be removed 2 and grouped new (family 27) nucleotide-diphosphosugar glycosyltransferases.It is important point out that sequences 27 members display limited similarities with those 2. It...
Ray Ming, Robert Paull, Qingyi Yu and colleagues report the genome sequences of two cultivated pineapple varieties one wild relative. Their analysis supports use as a reference for monocot comparative genomics provides insight into evolution crassulacean acid metabolism photosynthesis. Pineapple (Ananas comosus (L.) Merr.) is most economically valuable crop possessing (CAM), photosynthetic carbon assimilation pathway with high water-use efficiency, second important tropical fruit. We...
The fabrication of responsive photonic structures from cellulose nanocrystals (CNCs) that can operate in the entire visible spectrum is challenging due to requirements precise periodic modulation pitch size self‐assembled multilayer at length scale within wavelength light. surface charge density CNCs an important factor controlling chiral nematic structure dried solid CNC films. assembly poly(ethylene glycol) (PEG) together with into smaller domains results films uniform helical upon slow...
Significance Cellulose is the most abundant biopolymer on Earth, primarily formed by vascular plants, but also some bacteria. Bacterial extracellular polysaccharides, such as cellulose and alginate, are an important component of biofilms, which multicellular, usually sessile, aggregates Biofilms exhibit a greater resistance to antimicrobial treatments compared with isolated bacteria thus particular concern human health. synthases synthesize polymerizing UDP-activated glucose transport...
Oomycetes in the class Saprolegniomycetidae of Eukaryotic kingdom Stramenopila have evolved as severe pathogens amphibians, crustaceans, fish and insects, resulting major losses aquaculture damage to aquatic ecosystems. We sequenced 63 Mb genome fresh water pathogen, Saprolegnia parasitica. Approximately 1/3 assembled exhibits loss heterozygosity, indicating an efficient mechanism for revealing new variation. Comparison S. parasitica with plant pathogenic oomycetes suggests that during...
Cellulose, the important structural compound of cell walls, provides strength and rigidity to cells numerous organisms. Here, we functionally characterize four cellulose synthase genes (CesA) in oomycete plant pathogen Phytophthora infestans, causal agent potato (Solanum tuberosum) late blight. Three members this new protein family contain Pleckstrin homology domains form a distinct phylogenetic group most closely related synthases cyanobacteria. Expression all is coordinately upregulated...
Some of the most devastating plant and animal pathogens belong to oomycete class. The cell walls these microorganisms represent an excellent target for disease control, but their carbohydrate composition is elusive. We have undertaken a detailed wall analysis in 10 species from 2 major orders, Peronosporales Saprolegniales, thereby unveiling existence 3 clearly different types: type I devoid N-acetylglucosamine (GlcNAc) contains glucuronic acid mannose; II up 5% GlcNAc residues indicative...
An environmentally friendly approach was implemented for the production of nanocomposites with bactericidal activity, using bacterial cellulose (BC) nanofibers and chitin nanocrystals (ChNCs). The antibacterial activity ChNCs prepared by acid hydrolysis, TEMPO-mediated oxidation or partial deacetylation α-chitin powder assessed structure ChNC nanoparticles characterized X-ray diffraction, atomic force microscopy, solid-state 13C-NMR. partially deacetylated (D-ChNC) showed strongest 99 ± 1%...
Abstract Extracellular vesicles (EVs) are membranous that released by cells. In this study, the role of Endosomal Sorting Complex Required for Transport (ESCRT) machinery in biogenesis yeast EVs was examined. Knockout components ESCRT altered morphology and size as well decreased abundance EVs. contrast, strains with deletions cell wall biosynthesis genes, produced more than wildtype. Proteomic analysis highlighted depletion enrichment remodelling enzymes, glucan synthase subunit Fks1 chitin...
Significance Cellulose is an abundant natural polymer synthesized primarily by vascular plants in which it forms the load-bearing component of cell wall. It a linear glucose molecules membrane-embedded cellulose synthases that couple synthesis with its secretion across plasma membrane. Plants express multiple synthase isoforms are organized into large macromolecular assemblies varying composition likely responsible for aligning strands microfibrils. Here we show recombinantly expressed and...
An environmentally friendly, energy-efficient method for cellulose nanofibre (CNF) production from softwood holocellulose utilising oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs).
Detergent extracts of microsomal fractions from suspension cultured cells Rubus fruticosus (blackberry) were tested for their ability to synthesize in vitro sizable quantities cellulose UDP-glucose. Both Brij 58 and taurocholate effective yielded a substantial percentage microfibrils together with (1-->3)-beta-d-glucan (callose). The extracts, which did not require the addition Mg(2+), most efficient, yielding roughly 20% cellulose. This was characterized after callose removal by methylation...
Ultraviolet radiations have many detrimental effects in living organisms that challenge the stability and function of cellular structures. UV exposure also alters properties durability materials affects their lifetime. It is becoming increasingly important to develop new biocompatible environmentally friendly address these issues. Inspired by strategy developed fish, algae, microorganisms exposed confined ecosystems, we constructed novel UV-protective exclusively consist natural compounds....
Lignocellulosic biomass from softwood represents a valuable resource for the production of biofuels and bio-based materials as alternatives to traditional pulp paper products. Hemicelluloses constitute an extremely heterogeneous fraction plant cell wall, their molecular structures involve multiple monosaccharide components, glycosidic linkages, decoration patterns. The complete enzymatic hydrolysis wood hemicelluloses into monosaccharides is therefore complex biochemical process that...
Sequential combination of subcritical water and xylanolytic enzymes release bioactive hemicelluloses oligosaccharides from wheat bran with antioxidant activity.
The structure of fucoidan isolated from Laminaria hyperborea was elucidated and chemically tailored in order to obtain a clear structure-function relationship on bioactive properties with minimal amount variations among the tested molecules. Analysis revealed sugar composition 97.8% fucose 2.2% galactose. glycosidic linkages showed (1→3)-α-l-fuco-pyranose (31.9%) be dominant residue, followed by 1→2-linked (13.2%) 1→4-linked (7.7%) fuco-pyranose as well high degree branching (22.4%)....
The long-standing Acid Growth Theory of plant cell elongation posits that auxin promotes by stimulating wall acidification and thus expansin action. To date, the paucity pertinent genetic materials has precluded thorough analysis importance this concept in roots. recent isolation mutants model grass species Brachypodium distachyon with dramatically enhanced root due to increased cellular levels allowed us address question. We found primary transcriptomic effect associated elevated steady...