A5083285188- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Cellular Mechanics and Interactions
- Bacteriophages and microbial interactions
- NF-κB Signaling Pathways
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- interferon and immune responses
- Fibroblast Growth Factor Research
- Microbial infections and disease research
- Immunotherapy and Immune Responses
- Kruppel-like factors research
- T-cell and B-cell Immunology
- Cell death mechanisms and regulation
- Soil and Unsaturated Flow
- Genomics and Phylogenetic Studies
- Pancreatic function and diabetes
- Axon Guidance and Neuronal Signaling
- Metabolism, Diabetes, and Cancer
- Herpesvirus Infections and Treatments
- Galectins and Cancer Biology
- NMR spectroscopy and applications
- Virus-based gene therapy research
- TGF-β signaling in diseases
La Trobe University
2012-2023
The Royal Melbourne Hospital
1994-2009
The University of Melbourne
2002-2008
University College London
2006-2007
Ludwig Cancer Research
1990-2002
European Molecular Biology Laboratory
1998
European Molecular Biology Laboratory
1996
Walter and Eliza Hall Institute of Medical Research
1993-1994
Montreal Clinical Research Institute
1994
Ludwig Cancer Research
1990-1991
Invadopodia are actin-based projections enriched with proteases, which invasive cancer cells use to degrade the extracellular matrix (ECM). The Phox homology (PX)-Src (SH)3 domain adaptor protein Tks5 (also known as SH3PXD2A) cooperates Src tyrosine kinase promote invadopodia formation but underlying pathway is not clear. Here we show that phosphorylates at Y557, inducing it associate directly SH3-SH2 proteins Nck1 and Nck2 in invadopodia. mutants unable bind Nck reduced...
Abstract Extracellular vesicles (EVs) are membranous that released by cells. In this study, the role of Endosomal Sorting Complex Required for Transport (ESCRT) machinery in biogenesis yeast EVs was examined. Knockout components ESCRT altered morphology and size as well decreased abundance EVs. contrast, strains with deletions cell wall biosynthesis genes, produced more than wildtype. Proteomic analysis highlighted depletion enrichment remodelling enzymes, glucan synthase subunit Fks1 chitin...
Eph receptor tyrosine kinases and ephrins regulate morphogenesis in the developing embryo where they effect adhesion motility of interacting cells. Although scarcely expressed adult tissues, receptors are overexpressed a range tumours. In malignant melanoma, increased ephrin expression levels correlate with metastatic progression. We have examined cellular biochemical responses EphA3-expressing melanoma cell lines human epithelial kidney 293T cells to stimulation polymeric ephrin-A5 solution...
Mammalian hck, a member of the src family tyrosine kinases, is expressed predominantly in cells myeloid and B-lymphoid lineages. Using mutational analysis, we have investigated molecular basis two immunoreactive forms murine hck 56 59 kDa found numerous hemopoietic cell types. Our results indicate that translation p59hck initiates from CTG codon located 21 codons 5' an ATG utilized to generate p56hck. We provide evidence human isoforms are generated by same mechanism. Subcellular...
During germination of barley grains, the cell walls starchy endosperm are degraded by (1-->3,1-->4)-beta-glucanases (EC 3.2.1.73) secreted from aleurone and scutellar tissues. The complete sequence (1-->3,1-->4)-beta-glucanase isoenzyme II comprises 306 amino acids was determined sequencing nine tryptic peptides (110 residues) aligning them with acid deduced a cDNA clone encoding 291 NH(2)-terminal residues. Although no homology bacterial (1-->3)(1-->4)-beta-glucanase is apparent, close to...
Metastatic progression is the major cause of breast cancer-related mortality. By examining multiple syngeneic preclinical cancer models in mice lacking a functional type-I interferon receptor (Ifnar1(-/-) mice), we show that host-derived (IFN) signaling critical determinant metastatic spread independent primary tumor growth. In particular, bone metastasis can be accelerated Balb/c Ifnar1(-/-) bearing either 4T1 or 66cl4 orthotopic tumors and, for first time, present data showing development...
Abstract Fusobacterium nucleatum is an important oral bacterium that has been linked to the development of chronic diseases such as periodontitis and colorectal cancer. In periodontal disease, F. forms backbone polymicrobial biofilm in cancer implicated aetiology, metastasis chemotherapy resistance. The control this bacteria may be assisting treatment these diseases. With increased rates antibiotic resistance globally, there need for alternatives bacteriophages, which complement existing...
The increase in global warming has favoured growth of a range opportunistic environmental bacteria and allowed some these to become more pathogenic humans. Aeromonas hydrophila is one such organism. Surviving moist conditions temperate climates, have been associated with diseases humans, systemic infections can cause mortality up 46% cases. Their capacity form biofilms, carry antibiotic resistance mechanisms, survive disinfection, meant that they are not easily treated traditional methods....
Two lyn proteins of 56 and 53 kDa have been observed in immunoprecipitates from a variety murine human cell lines tissues. We report the cloning nucleotide sequence two distinct cDNAs isolated an FDC-P1 cDNA library. One cDNAs, designated lyn11, encodes protein which shares 96% similarity with lyn. The other cDNA, lyn12, kDa. differ presence or absence 21-amino-acid located 24 amino acids C terminal translational initiation codon. Using RNase protection analysis, we identified mRNAs...
Tks5 is a scaffold protein and Src substrate involved in cell migration matrix degradation through its essential role invadosome formation function. We have previously described that fundamental for zebrafish neural crest vivo. In the present study, we sought to investigate function of mammalian development by analyzing mice mutant sh3pxd2a, gene encoding Tks5. Homozygous disruption sh3pxd2a gene-trapping mouse resulted neonatal death presence complete cleft secondary palate. Interestingly,...
Mitoxantrone was efficiently encapsulated within cucurbit[8]uril in a 2:1 complex where the two mitoxantrone molecules were symmetrically located through both portals of cage. The novel facilitates increased uptake mouse breast cancer cells and decreases toxicity drug healthy mice. In an orthotopic model metastatic still maintains vivo anticancer activity compared to free drug, yet provides statistically significant increase survival these mice conventional treatment. This new low...
Crocodilepox virus is a large dsDNA belonging to the genus Crocodylidpoxvirus, which infects wide range of host species in order Crocodylia worldwide. Here, we present genome sequences for novel saltwater crocodilepox virus, with two subtypes (SwCRV-1 and -2), isolated from Australian crocodile. Affected belly skins juvenile crocodiles were used sequence complete viral genomes, perform electron microscopic analysis that visualized immature mature virions. Analysis SwCRV genomes showed high...
Triple-negative breast cancer (TNBC) represents 10-20% of all human ductal adenocarcinomas and has a poor prognosis relative to other subtypes, due the high propensity develop distant metastases. Hence, new molecular targets for therapeutic intervention are needed TNBC. We recently conducted rigorous phenotypic genomic characterization four isogenic populations MDA-MB-231 triple-negative cells that possess range intrinsic spontaneous metastatic capacities in vivo, ranging from nonmetastatic...
ARAP3 is a GTPase activating protein (GAP) for Rho and Arf GTPases that implicated in phosphoinositide 3-kinase (PI 3-kinase) signalling pathways controlling lamellipodia formation actin stress fibre assembly. We have identified as phosphorylated target of tyrosine kinases. In cells, was when co-expressed with Src-family kinases (SFKs), upon stimulation growth factors during adhesion to the extracellular matrix (ECM) substrate fibronectin. Adhesion-induced phosphorylation suppressed by...
A murine embryonic cDNA library was screened for potential substrates of the Src family kinase, Lyn, using a phosphorylation-screening strategy. One that we identified encodes Dok-related protein (DokR), with homology to p62(dok) (Dok), and members insulin receptor substrate-1 proteins. Analysis tissue extracts DokR-specific antisera revealed DokR is expressed at highest levels in lymphoid tissues. Co-expression FLAG epitope-tagged form (FLAG-DokR) Lyn kidney 293T cells resulted constitutive...