A5010360090- Polysaccharides and Plant Cell Walls
- Biofuel production and bioconversion
- Food composition and properties
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Plant nutrient uptake and metabolism
- Fermentation and Sensory Analysis
- Marine and coastal plant biology
- Seaweed-derived Bioactive Compounds
- Polysaccharides Composition and Applications
- Algal biology and biofuel production
- Phytase and its Applications
- Wheat and Barley Genetics and Pathology
- Microbial Metabolism and Applications
- Insect Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Genetic and Environmental Crop Studies
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- Malaria Research and Control
- Receptor Mechanisms and Signaling
- Plant tissue culture and regeneration
- Ocean Acidification Effects and Responses
- Infant Nutrition and Health
- Amino Acid Enzymes and Metabolism
The University of Adelaide
2015-2024
Australian Wine Research Institute
2004-2024
ARC Centre of Excellence in Plant Energy Biology
2013-2022
Australian Research Council
2015-2019
Australian Centre for Plant Functional Genomics
2005-2010
Wine Australia
2008
Molecular interactions between wall polysaccharides, which include cellulose and a range of noncellulosic polysaccharides such as xyloglucans (1,3;1,4)-beta-D-glucans, are fundamental to cell properties. These have been assumed be noncovalent in nature most cases. Here we show that highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), member the GH16 group glycoside hydrolases, catalyzes vitro formation covalent linkages cellulosic substrates...
Cell walls are crucial for the integrity and function of all land plants central importance in human health, livestock production, as a source renewable bioenergy. Many enzymes that mediate biosynthesis cell wall polysaccharides encoded by members large cellulose synthase (CesA) gene superfamily. Here, we analyzed 29 sequenced genomes 17 transcriptomes to revise phylogeny CesA superfamily angiosperms. Our results identify ancestral clusters predate monocot-eudicot divergence reveal several...
Plant biomass from different species is heterogeneous, and this diversity in composition can be mined to identify materials of value fuel chemical industries. Agave produces high yields energy-rich biomass, the sugar-rich stem tissue has traditionally been used make alcoholic beverages. Here, compositions americana tequilana leaves are determined, particularly context bioethanol production. leaf cell wall polysaccharide content was characterized by linkage analysis, non-cellulosic...
An α-l-arabinofuranosidase and a β-d-xylosidase, designated ARA-I XYL, respectively, have been purified about 1,000-fold from extracts of 5-day-old barley (Hordeum vulgare L.) seedlings using ammonium sulfate fractional precipitation, ion exchange chromatography, chromatofocusing, size-exclusion chromatography. The has an apparent molecular mass 67 kDa isoelectric point 5.5, its catalytic efficiency during hydrolysis 4′-nitrophenyl α-l-arabinofuranoside is only slightly higher than...
The nutritional values associated with the cell walls of cereal endosperm flours are due to a combination solubilized arabinoxylan and (1-3,1-4)-β-d-glucan as well residual nonsolubilized wall material. In order investigate structure-nutrition relationships, an appropriate method for complete functional structural characterization polysaccharides in various is described. This involves separation soluble polymers fraction without using organic solvents, fractionation into arabinoxylan-...
Arabinoxylans are one group of dietary fiber components in cereal grains, and specific health benefits have been linked with their molecular fine structures hence physicochemical properties such as solubility aqueous media. To characterize the quality for functional foods, starchy endosperm bran fractions from 11 durum wheat lines were analyzed total water-soluble arabinoxylans, (1,3;1,4)-β-glucan, bound ferulic acid. The arabinoxylan contents ranged to 16.4% (w/w) 1.5 1.8% endosperm. Of 37%...
The C‐type natriuretic peptide from the platypus venom (OvCNP) exists in two forms, OvCNPa and OvCNPb, whose amino acid sequences are identical. Through use of nuclear magnetic resonance, mass spectrometry, peptidase digestion studies, we discovered that OvCNPb incorporates a D ‐amino at position 2 primary structure. Peptides containing have been found lower forms organism, but this report is first for biologically active mammal. result implies existence specific isomerase converts an L...
In barley endosperm arabinoxylan (AX) is the second most abundant cell wall polysaccharide and in wheat it starchy walls of grain. AX one main contributors to grain dietary fibre content providing several health benefits including cholesterol glucose lowering effects, antioxidant activities. Due its complex structural features, might also affect downstream applications malting brewing. Using a high pressure liquid chromatography (HPLC) method we quantified amounts mature 128 spring 2-row...
Xylans are the most abundant non-cellulosic polysaccharide found in plant cell walls. A diverse range of xylan structures influence tissue function during growth and development. Despite abundance xylans nature, details genes biochemical pathways controlling their biosynthesis lacking. In this study we have utilized natural variation within Plantago genus to examine heteroxylan composition structure seed coat mucilage. Compositional assays were combined with analysis glycosyltransferase...
Abstract In cereals, the presence of soluble polysaccharides including (1,3;1,4)‐β‐glucan has downstream implications for human health, animal feed and biofuel applications. Sorghum bicolor (L.) Moench is a versatile crop, but there are limited reports regarding content such polysaccharides. Here, amount present in sorghum tissues was measured using Megazyme assay. Very low amounts were grain, ranging from 0.16%–0.27% (w/w), while greater quantity vegetative at 0.12–1.71% (w/w). The fine...
Cellulose synthase-like F6 (CslF6) genes encode polysaccharide synthases responsible for (1,3;1,4)-β-glucan biosynthesis in cereal grains. However, it is not clear how both (1,3)- and (1,4)-linkages are incorporated into a single chain the frequency arrangement of two linkage types that define fine structure controlled. Through transient expression Nicotiana benthamiana leaves, CSLF6 orthologs from different species were shown to mediate synthesis (1,3;1,4)-β-glucans with very structures....
As a significant component of monocot cell walls, (1,3;1,4)-β-glucan has conclusively been shown to be synthesized by the cellulose synthase-like F6 protein. In this study, we investigated synthetic activity other members barley (Hordeum vulgare) CslF gene family using heterologous expression. expected, majority genes encode proteins that are capable synthesizing detectable levels (1,3;1,4)-β-glucan. However, overexpression HvCslF3 and HvCslF10 resulted in synthesis novel linear glucoxylan...
UGE (UDP-Glc 4-epimerase or UDP-Gal 4-epimerase; EC 5.1.3.2) catalyses the interconversion of and UDP-Glc. Both nucleotide sugars act as activated sugar donors for biosynthesis cell wall polysaccharides such cellulose, xyloglucans, (1,3;1,4)-beta-D-glucan pectins, together with other biologically significant compounds including glycoproteins glycolipids. Three members HvUGE (barley UGE) gene family, designated HvUGE1, HvUGE2 HvUGE3, have been characterized. Q-PCR (quantitative real-time PCR)...
A family GH5 (family 5 glycoside hydrolase) (1,4)-β-D-mannan endohydrolase or β-D-mannanase (EC 3.2.1.78), designated HvMAN1, has been purified 300-fold from extracts of 10-day-old barley (Hordeum vulgare L.) seedlings using ammonium sulfate fractional precipitation, followed by ion exchange, hydrophobic interaction and size-exclusion chromatography. The HvMAN1 is a relatively unstable enzyme with an apparent molecular mass 43 kDa, pI 7.8 pH optimum 4.75. releases Man (mannose...
A family 16 glycoside hydrolase, xyloglucan xyloglucosyl transferase (EC 2.4.1.207), also known as endotransglycosylase (XET), and designated isoenzyme HvXET6, was purified approximately 400‐fold from extracts of young barley seedlings. The complete amino acid sequence HvXET6 deduced the nucleotide a near full‐length cDNA, in combination with tryptic peptide mapping. An additional five to six isoforms or post‐translationally modified XET enzymes were detected crude seedling barley. expressed...