A5066070550- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Ion Transport and Channel Regulation
- Biofuel production and bioconversion
- Membrane-based Ion Separation Techniques
- Food composition and properties
- Plant Molecular Biology Research
- Magnetic and Electromagnetic Effects
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Plant responses to water stress
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Bioenergy crop production and management
- Microbial Metabolites in Food Biotechnology
- Plant Surface Properties and Treatments
- Silicon Effects in Agriculture
- Plant Genetic and Mutation Studies
- Lignin and Wood Chemistry
- Diverse Educational Innovations Studies
- Climate change impacts on agriculture
- Lichen and fungal ecology
- Ginseng Biological Effects and Applications
- Island Studies and Pacific Affairs
Australian National University
2019-2025
The University of Adelaide
2013-2023
Australian Research Council
2013-2023
Australian Wine Research Institute
2012-2023
ARC Centre of Excellence in Plant Energy Biology
2013-2021
ARC Centre of Excellence for Transformative Meta-Optical Systems
2021
Gyeongsang National University
2019
Australian Centre for Plant Functional Genomics
2007-2015
Plant Industry
2007-2014
Commonwealth Scientific and Industrial Research Organisation
2008-2014
Two major genes for Na+ exclusion in durum wheat, Nax1 and Nax2, that were previously identified as the transporters TmHKT1;4-A2 TmHKT1;5-A, transferred into bread wheat order to increase its capacity restrict accumulation of leaves. The crossed from tetraploid (Triticum turgidum ssp. durum) hexaploid aestivum) by interspecific crossing marker-assisted selection plants containing one or both genes. decreased leaf blade concentration 50%, Nax2 it 30%, together 60%. signature phenotype Nax1,...
Bread wheat (Triticum aestivum) has a greater ability to exclude Na+ from its leaves and is more salt tolerant than durum turgidum L. subsp. [Desf.]). A novel wheat, Line 149, was found contain major gene for exclusion, Nax2, which removes the xylem in roots leads high K+-to-Na+ ratio leaves. Nax2 mapped distal region on chromosome 5AL based linkage microsatellite markers. The locus coincides with putative transporter, HKT1;5 (HKT8). homoeologous 4DL containing exclusion bread Kna1. member...
Bread wheat (Triticum aestivum L.) has a major salt tolerance locus, Kna1, responsible for the maintenance of high cytosolic K(+) /Na(+) ratio in leaves stressed plants. The Kna1 locus encompasses large DNA fragment, distal 14% chromosome 4DL. Limited recombination been observed at this making it difficult to map genetically and identify causal gene. Here, we decipher function TaHKT1;5-D, candidate gene underlying locus. Transport studies using heterologous expression systems Saccharomyces...
Silicon application can improve productivity outcomes for salt stressed plants. Here, we describe how strawberry plants respond to treatments including various combinations of stress and nano-silicon dioxide, assess whether dioxide improves plant tolerance stress. Strawberry were treated with (0, 25 or 50 mM NaCl), the applied before 100 mg L−1) after (0 flowering. The reduced biomass, chlorophyll content, leaf relative water content (RWC) as expected. Relative control (no NaCl) had 10%...
The aquaporin AtPIP2;1 is an abundant plasma membrane intrinsic protein in Arabidopsis thaliana that implicated stomatal closure, and highly expressed membranes of root epidermal cells. When Xenopus laevis oocytes, increased water permeability induced a non-selective cation conductance mainly associated with Na+ . A mutation the pore, G103W, prevented both ionic PIP2;1. Co-expression AtPIP1;2 but abolished conductance. AtPIP2;2 (93% identical to AtPIP2;1) similarly not was inhibited by...
Plant biomass from different species is heterogeneous, and this diversity in composition can be mined to identify materials of value fuel chemical industries. Agave produces high yields energy-rich biomass, the sugar-rich stem tissue has traditionally been used make alcoholic beverages. Here, compositions americana tequilana leaves are determined, particularly context bioethanol production. leaf cell wall polysaccharide content was characterized by linkage analysis, non-cellulosic...
Under saline conditions, higher plants restrict the accumulation of chloride ions (Cl–) in shoot by regulating their transfer from root symplast into xylem-associated apoplast. To identify molecular mechanisms underpinning this phenomenon, we undertook a transcriptional screen salt stressed Arabidopsis (Arabidopsis thaliana) roots. Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified candidate gene involved Cl– xylem loading Nitrate transporter 1/Peptide Transporter family...
Aquaporins function as water and neutral solute channels, signaling hubs, disease virulence factors, metabolon components. We consider plant aquaporins that transport ions compared to some animal counterparts. These are candidates for important, yet unidentified, cation anion channels in plasma, tonoplast, symbiotic membranes. For those individual isoforms ions, water, gases, the permeability spans 12 orders of magnitude. This requires tight regulation selectivity via protein interactions...
Excessive soil salinity diminishes crop yield and quality. In a previous study in tomato, we identified two closely linked genes encoding HKT1-like transporters, HKT1;1 HKT1;2, as candidate for major quantitative trait locus (kc7.1) related to shoot Na+ /K+ homeostasis - salt tolerance using populations of recombinant inbred lines (RILs). Here, determine the effectiveness these conferring improved by near-isogenic (NILs) that were homozygous either Solanum lycopersicum allele (NIL17) or...
The global need for increased food production means that agriculture is moving into regions with lower rainfall and saline soils, which occupy over 6% of the world land area (Munns, 2005). aim this workshop was to work towards completing an energy budget mechanisms salinity tolerance in crop plants, as a guide most cost-effective breeding strategies increasing salt yield important crops. discussions took account supply from mitochondria chloroplasts, demands transport water, Na+, Cl− K+,...
Aquaporins (AQPs) are known to facilitate water and solute fluxes across barrier membranes. An increasing number of AQPs being found serve as ion channels. Ion permeability selected plant animal (plant Arabidopsis thaliana AtPIP2;1, AtPIP2;2, AtPIP2;7, human Homo sapiens HsAQP1, rat Rattus norvegicus RnAQP4, RnAQP5, fly Drosophila melanogaster DmBIB) were expressed in Xenopus oocytes examined chelator-buffered salines evaluate the effects divalent cations (Ca2+, Mg2+, Ba2+ Cd2+) on ionic...
The phosphorylation state of two serine residues within the C-terminal domain AtPIP2;1 (S280, S283) regulates its plasma membrane localization in response to salt and osmotic stress. Here, we investigated whether S280 S283 also influence facilitated water cation transport. A series single double phosphomimic phosphonull mutants were tested heterologous systems. In Xenopus laevis oocytes, S280D, S283D, S280D/S283D had significantly greater ion conductance for Na+ K+ , whereas S280A mutant...
A fundamental limitation of photosynthetic carbon fixation is the availability CO 2 . In C 4 plants, primary carboxylation occurs in mesophyll cytosol, and little known about role diffusion facilitating photosynthesis. We have examined expression, localization, functional selected plasma membrane intrinsic aquaporins (PIPs) from Setaria italica (foxtail millet) discovered that SiPIP2;7 -permeable. When ectopically expressed cells viridis (green foxtail), was localized to caused no marked...
Engineering membrane transporters to achieve desired functionality is reliant on availability of experimental data informing structure-function relationships and intelligent design. Plant aquaporin (AQP) isoforms are capable transporting diverse substrates such as signaling molecules, nutrients, metalloids, gases, well water. AQPs can act multifunctional channels their transport function many factors, with few studies having assessed specific for multiple substrates.High-throughput yeast...
Sorghum bicolor is a genetically diverse C4 monocotyledonous species, encompassing varieties capable of producing high grain yields as well sweet types which accumulate soluble sugars (predominantly sucrose) within their stems to concentrations. Sucrose produced in leaves (sources) enters the phloem and transported regions growth storage (sinks). It likely that sucrose transporter (SUT) proteins play pivotal roles loading delivery sinks all ecotypes. Six SUTs are present published genome,...
Societal Impact Statement Rare earth elements (REEs) are critical resources required to achieve net‐zero carbon emission targets and energy security. However, rising demand for REEs coupled with significant extraction processing challenges geopolitical risks restricts access REE resources. innovations that reduce hazardous waste generation improve efficiency needed. Plants, some microorganisms, can harness properties enhance their metabolic processes physiological functions. Exploration...
A major hindrance to the development of high yielding biofuel feedstocks is ability rapidly assess large populations for fermentable sugar yields. Whilst recent advances have outlined methods rapid assessment biomass saccharification efficiency, none take into account total biomass, or soluble fraction plant. Here we present a holistic high-throughput methodology assessing sweet Sorghum bicolor at 10 days post-anthesis yields including stalk concentrations, and cell wall efficiency.A...