A5056794307- Ion Transport and Channel Regulation
- Natural product bioactivities and synthesis
- Membrane-based Ion Separation Techniques
- Ion channel regulation and function
- Plant Stress Responses and Tolerance
- Phytochemical Studies and Bioactivities
- Bioactive natural compounds
- Plant nutrient uptake and metabolism
- Magnetic and Electromagnetic Effects
- Galectins and Cancer Biology
- Protein Tyrosine Phosphatases
- Gut microbiota and health
- Cancer Immunotherapy and Biomarkers
- Cardiac electrophysiology and arrhythmias
- Magnesium in Health and Disease
- ATP Synthase and ATPases Research
- Microbial Metabolites in Food Biotechnology
- Neuroscience and Neural Engineering
- Chromatography in Natural Products
- Erythrocyte Function and Pathophysiology
- Toxin Mechanisms and Immunotoxins
- Studies on Chitinases and Chitosanases
- Immune Response and Inflammation
- Cancer therapeutics and mechanisms
- Photochromic and Fluorescence Chemistry
The University of Adelaide
2017-2025
Basil Hetzel Institute
2025
Queen Elizabeth Hospital
2025
Australian Wine Research Institute
2024
Australian National University
2020
Australian Research Council
2018
Aquaporins (AQPs) are known to facilitate water and solute fluxes across barrier membranes. An increasing number of AQPs being found serve as ion channels. Ion permeability selected plant animal (plant Arabidopsis thaliana AtPIP2;1, AtPIP2;2, AtPIP2;7, human Homo sapiens HsAQP1, rat Rattus norvegicus RnAQP4, RnAQP5, fly Drosophila melanogaster DmBIB) were expressed in Xenopus oocytes examined chelator-buffered salines evaluate the effects divalent cations (Ca2+, Mg2+, Ba2+ Cd2+) on ionic...
Traditional Chinese Medicines are promising sources of new agents for controlling cancer metastasis. Compound Kushen Injection (CKI), prepared from medicinal plants Sophora flavescens and Heterosmilax chinensis, disrupts cell cycle induces apoptosis in breast cancer; however, effects on migration invasion remained unknown. CKI, fractionated mixtures, isolated components were tested assays with colon (HT-29, SW-480, DLD-1), brain (U87-MG, U251-MG), (MDA-MB-231) lines. Human embryonic kidney...
BackgroundThe prognostic value of tumor-infiltrating lymphocytes (TILs) assessed by machine learning algorithms in melanoma patients has been previously demonstrated but not widely adopted the clinic. We evaluated objective automated electronic TILs (eTILs) quantification to define a subset with low risk relapse after surgical treatment.MethodsWe analyzed data for 785 from 5 independent cohorts multiple institutions validate our previous finding that TIL score is clinically-localized primary...
Triple-negative breast cancer (TNBC) is an aggressive subtype with limited treatment options and high resistance to chemotherapy. Doxorubicin commonly used, but its efficacy by variable sensitivity resistance. Bacopaside II, a saponin compound, has shown anti-cancer potential. This study evaluates the effects of doxorubicin bacopaside both individually in combination, across TNBC subtypes explore mechanisms enhanced drug efficacy. The growth-inhibitory II were assessed four cell lines. IC50...
The breast tissue microbiome has been increasingly recognized as a potential contributor to cancer development and progression. However, inconsistencies in microbial composition across studies have hindered the identification of definitive signatures. To address these gaps, we conducted systematic review meta-analysis 11 employing 16S rRNA sequencing characterize bacterial 1,260 fresh samples, including normal, mastitis-affected, benign, cancer-adjacent, cancerous tissues. We also...
Aquaporin-1 (AQP1) has been proposed as a dual water and cation channel that when upregulated in cancers enhances cell migration rates; however, the mechanism remains unknown. Previous work identified AqB011 an inhibitor of gated human AQP1 conductance, bacopaside II blocker pores. In two colorectal adenocarcinoma lines, high levels transcript were confirmed HT29, low SW480 cells, by quantitative PCR (polymerase chain reaction). Comparable differences membrane protein demonstrated...
Aquaporin-1 (AQP1) dual water and ion channels enhance migration invasion when upregulated in leading edges of certain classes cancer cells. Work here identifies structurally related furan compounds as novel inhibitors AQP1 channels. 5-Hydroxymethyl-2-furfural (5HMF), a component natural medicinal honeys, three compounds, 5-nitro-2-furoic acid (5NFA), 5-acetoxymethyl-2-furaldehyde (5AMF), methyl-5-nitro-2-furoate (M5NF), were analyzed for effects on channel activities human expressed...
This is the first work to use a newly designed Li<sup>+</sup>-selective photoswitchable probe Sabrina Heng Lithium (SHL) in living colon cancer cells noninvasively monitor cation channel activity real time by appearance of lithium hot spots detected confocal microscopy. Punctate Li<sup>+</sup> are clustered lamellipodial leading edges HT29 human and colocalized with aquaporin-1 (AQP1) channels. AQP1 dual water cyclic-nucleotide-gated located lamellipodia essential for rapid cell migration...
Aquaporins are integral proteins that facilitate the transmembrane transport of water and small solutes. In addition to enabling flux, mammalian Aquaporin-1 (AQP1) channels activated by cyclic GMP can carry non-selective monovalent cation currents, selectively blocked arylsulfonamide compounds AqB007 (IC50 170 μM) AqB011 14 μM). silico models suggested ligand docking might involve cytoplasmic loop D (between AQP1 domains 4 5), but predicted site interaction remained be tested. Work here...
Abstract We used computational and experimental biology approaches to identify candidate mechanisms of action aTraditional Chinese Medicine, Compound Kushen Injection (CKI), in a breast cancer cell line (MDA-MB-231). Because CKI is complex mixture plant secondary metabolites, we high-performance liquid chromatography (HPLC) fractionation reconstitution approach define chemical fractions required for induce apoptosis. The initial separated major from minor compounds, it showed that compounds...
Abstract Background and Purpose Due to the complexity of traditional Chinese medicine based on complex mixtures natural products their multi-target mechanism action, discovery validation relevant targets have always been challenging. In previous studies, using transcriptomic methods Compound Kushen Injection (CKI) as a model drug, we identified multiple pathways target genes through which CKI exerts its pharmacological effects. Therefore, wished verify these by perturbing those genetic...
In sickle cell disease (SCD), the pathological shift of red blood cells (RBCs) into distorted morphologies under hypoxic conditions follows activation a cationic leak current (Psickle) and dehydration. Prior work showed sickling was reduced by 5-hydroxylmethyl-2-furfural (5-HMF), which stabilized mutant hemoglobin also blocked Psickle in RBCs, though molecular basis this 5-HMF-sensitive cation remained mystery. Work here is first to test hypothesis that Aquaporin-1 (AQP1) channels contribute...
Abstract Cancer metastasis is a major cause of death. Traditional Chinese medicines (TCM) are promising sources new anti-metastatic agents. Compound Kushen Injection (CKI), extracted from medicinal plants, (Sophora flavescens) and Baituling (Heterosmilax chinensis), contains mixture alkaloids flavonoids known to disrupt cell cycle induce apoptosis in breast cancer (MCF7). However, effects on migration invasion have remained unknown. CKI, fractionated mixtures, single identified components...
Abstract We have used computational and experimental biology approaches to identify candidate mechanisms of action a traditional Chinese medicine. Compound Kushen Injection (CKI), in breast cancer cell line which CKI causes apoptosis. Because is complex mixture plant secondary metabolites, we high-performance liquid chromatography (HPLC) fractionation reconstitution approach define chemical fractions required for induce apoptosis MDA-MB-231 cells. Our initial separated major from minor...
Plant cation-chloride-cotransporters (CCC) are proposed to be Na+–K+–2Cl–transporting proteins, although phylogenetically they closer K+-Cl–cotransporters (KCC). Conserved features of plant CCC and animal KCC include the presence predicted K+ Cl– binding sites, absence a Na+ site. Here, we investigated grapevine (Vitis vinifera L.) VvCCC using protein structural modelling heterologous expression. Our data that 3D folds were more similar DrNKCC1, but sequences ion sites resembled those hKCC1....
Abstract Aquaporin (AQP) channels found in all domains of life are transmembrane proteins which mediate passive transport water, glycerol, signaling molecules, metabolites, and charged solutes. Discovery new classes ion-conducting AQP has been slow, likely reflecting time- labor-intensive methods required for traditional electrophysiology. Work here defines a sensitive mass-throughput system detecting ion channels, identified by rescue cell growth the K+-transport-defective yeast strain...
Plant cation-chloride cotransporters (CCCs) are proposed to be Na
Background: Triple-negative breast cancer (TNBC) is a highly aggressive subtype with limited treatment options and high drug resistance. Doxorubicin commonly used for TNBC, but variable sensitivity across subtypes, resistance development toxicity limit its effi-cacy. Bacopaside II, saponin compound, has shown anti-cancer potential. This study assesses the effects of doxorubicin bacopaside individually combined, TNBC subtypes to explore mechanisms sensitivity, resistance, enhanced efficacy....