A5050194549- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Ion Transport and Channel Regulation
- Membrane-based Ion Separation Techniques
- Plant Molecular Biology Research
- Seed Germination and Physiology
- Aluminum toxicity and tolerance in plants and animals
- Magnetic and Electromagnetic Effects
- Food composition and properties
- Parathyroid Disorders and Treatments
- Glycosylation and Glycoproteins Research
- Infant Nutrition and Health
- Magnesium in Health and Disease
- ATP Synthase and ATPases Research
- Archaeological Research and Protection
- Genetics and Plant Breeding
- Cancer Research and Treatments
- Molecular Sensors and Ion Detection
- Trace Elements in Health
- Phytochemical Studies and Bioactivities
- Chemical Synthesis and Characterization
- Advanced Proteomics Techniques and Applications
The University of Adelaide
2015-2023
ARC Centre of Excellence in Plant Energy Biology
2015-2023
Australian Research Council
2016-2023
Australian Wine Research Institute
2017-2023
Australian Centre for Plant Functional Genomics
2015-2016
The aquaporin AtPIP2;1 is an abundant plasma membrane intrinsic protein in Arabidopsis thaliana that implicated stomatal closure, and highly expressed membranes of root epidermal cells. When Xenopus laevis oocytes, increased water permeability induced a non-selective cation conductance mainly associated with Na+ . A mutation the pore, G103W, prevented both ionic PIP2;1. Co-expression AtPIP1;2 but abolished conductance. AtPIP2;2 (93% identical to AtPIP2;1) similarly not was inhibited by...
Organelle-nuclear retrograde signaling regulates gene expression, but its roles in specialized cells and integration with hormonal remain enigmatic. Here we show that the SAL1-PAP (3′-phosphoadenosine 5′- phosphate) pathway interacts abscisic acid (ABA) to regulate stomatal closure seed germination Arabidopsis. Genetically or exogenously manipulating PAP bypasses canonical components ABA Insensitive 1 (ABI1) Open Stomata (OST1); priming an alternative restores ABA-responsive ROS bursts, ion...
Under saline conditions, higher plants restrict the accumulation of chloride ions (Cl–) in shoot by regulating their transfer from root symplast into xylem-associated apoplast. To identify molecular mechanisms underpinning this phenomenon, we undertook a transcriptional screen salt stressed Arabidopsis (Arabidopsis thaliana) roots. Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified candidate gene involved Cl– xylem loading Nitrate transporter 1/Peptide Transporter family...
Salinity tolerance is correlated with shoot chloride (Cl(-)) exclusion in multiple crops, but the molecular mechanisms of long-distance Cl(-) transport are poorly defined. Here, we characterize planta role AtSLAH1 (a homologue slow type anion channel-associated 1 (SLAC1)). This protein, localized to plasma membrane root stelar cells, has its expression reduced by salt or ABA, which key predictions for a protein involved loading into xylem. Artificial microRNA knockdown mutants had...
Aquaporins function as water and neutral solute channels, signaling hubs, disease virulence factors, metabolon components. We consider plant aquaporins that transport ions compared to some animal counterparts. These are candidates for important, yet unidentified, cation anion channels in plasma, tonoplast, symbiotic membranes. For those individual isoforms ions, water, gases, the permeability spans 12 orders of magnitude. This requires tight regulation selectivity via protein interactions...
The accumulation of high concentrations chloride (Cl–) in leaves can adversely affect plant growth. When comparing different varieties the same Cl– sensitive species those that exclude relatively more from their shoots tend to perform better under saline conditions; however, molecular mechanisms involved maintaining low shoot remain largely undefined. Recently, it was shown NRT1/PTR Family 2.4 protein (NPF2.4) loads into root xylem, which affects Arabidopsis shoots. Here we characterize...
Aquaporins (AQPs) are known to facilitate water and solute fluxes across barrier membranes. An increasing number of AQPs being found serve as ion channels. Ion permeability selected plant animal (plant Arabidopsis thaliana AtPIP2;1, AtPIP2;2, AtPIP2;7, human Homo sapiens HsAQP1, rat Rattus norvegicus RnAQP4, RnAQP5, fly Drosophila melanogaster DmBIB) were expressed in Xenopus oocytes examined chelator-buffered salines evaluate the effects divalent cations (Ca2+, Mg2+, Ba2+ Cd2+) on ionic...
The phosphorylation state of two serine residues within the C-terminal domain AtPIP2;1 (S280, S283) regulates its plasma membrane localization in response to salt and osmotic stress. Here, we investigated whether S280 S283 also influence facilitated water cation transport. A series single double phosphomimic phosphonull mutants were tested heterologous systems. In Xenopus laevis oocytes, S280D, S283D, S280D/S283D had significantly greater ion conductance for Na+ K+ , whereas S280A mutant...
Engineering membrane transporters to achieve desired functionality is reliant on availability of experimental data informing structure-function relationships and intelligent design. Plant aquaporin (AQP) isoforms are capable transporting diverse substrates such as signaling molecules, nutrients, metalloids, gases, well water. AQPs can act multifunctional channels their transport function many factors, with few studies having assessed specific for multiple substrates.High-throughput yeast...
Plant cation-chloride cotransporters (CCCs) have been implicated in conferring salt tolerance. They are predicted to improve shoot exclusion by directly catalyzing the retrieval of sodium (Na+) and chloride (Cl−) ions from root xylem. We investigated whether grapevine (Vitis vinifera [Vvi]) CCC has a role tolerance cloning functionally characterizing gene cultivar Cabernet Sauvignon. Amino acid sequence analysis revealed that VviCCC shares high degree similarity with other plant CCCs. A...
Abstract During plant growth, sodium (Na + ) in the soil is transported via xylem from root to shoot. While excess Na toxic most plants, non-toxic concentrations have been shown improve crop yields under certain conditions, such as when K low. We quantified grain across a barley genome-wide association study panel grown non-saline conditions and identified variants of Class 1 HIGH-AFFINITY-POTASSIUM-TRANSPORTER ( HvHKT1;5 )-encoding gene responsible for content variation these conditions. A...
Abstract Improving salinity tolerance in the most widely cultivated cereal, bread wheat ( Triticum aestivum L.), is essential to increase grain yields on saline agricultural lands. A Portuguese landrace, Mocho de Espiga Branca accumulates up sixfold greater leaf and sheath sodium (Na + ) than two Australian cultivars, Gladius Scout, under salt stress hydroponics. Despite high Na concentrations, maintained similar compared Scout. naturally occurring single nucleotide substitution was...
Some plasma membrane intrinsic protein (PIP) aquaporins can facilitate ion transport. Here we report that one of the 12 barley PIPs (PIP1 and PIP2) tested, HvPIP2;8, facilitated cation transport when expressed in Xenopus laevis oocytes. HvPIP2;8-associated currents were detected with Na+ K+, but not Cs+, Rb+, or Li+, was inhibited by Ba2+, Ca2+, Cd2+ to a lesser extent Mg2+, which also interacted Ca2+. Currents reduced presence Li+ relative alone. Five HvPIP1 isoforms co-expressed HvPIP2;8...
Dynamic changes in aquaporin gene expression occur during seed germination. One example is the ~30-fold increase Arabidopsis thaliana PIP2;1 transcripts within 24h of imbibition. To investigate whether AtPIP2;1 can influence germination wild-type Columbia-0, single (Atpip2;1 ) and double (Atpip2;1-Atpip2;2 loss-of-function mutants, along with transgenic 2x35S::AtPIP2;1 over-expressing (OE) lines null-segregant controls, were examined. The various genotypes germinated control saline (75mM...
Abstract Plant aquaporins have many more functions than just transporting water. Within the diversity of plant are isoforms capable signaling molecules, nutrients, metalloids and gases. It is established that aquaporin substrate discrimination depends on combinations factors such as solute size, pore size polarity, post-translational protein modifications. But our understanding relationships between variation in structures implications for permeability limited. High-throughput yeast-based...
Abstract Improving salinity tolerance in the most widely cultivated cereal, bread wheat ( Triticum aestivum L.), is essential to increase grain yields on saline agricultural lands. A Portuguese landrace, Mocho de Espiga Branca accumulates up 6 folds greater leaf and sheath sodium (Na + ) than two Australian cultivars, Gladius Scout, under salt stress. Despite high Na concentrations, maintained similar compared Scout. naturally occurring single nucleotide substitution was identified gene...
ABSTRACT We quantified grain sodium (Na + ) content across a barley GWAS panel grown under optimal conditions. identified strong association with region containing two low and one high Na accumulating haplotypes of Class 1 HIGH-AFFINITY POTASSIUM TRANSPORTER (HKT1;5) known to be involved in regulating plant homeostasis. The exhibited an average 1.8-fold difference content. show that L189P substitution disrupts transport the lines, disturbs plasma membrane localisation typical HKT1;5 induces...
The phosphorylation states of two serine residues within the C-terminal domain AtPIP2;1 (S280, S283) regulate its trafficking to plasma membrane in response salt and osmotic stress. Here we investigated whether S280 S283 also influence facilitated water cation transport. A series single double phospho-mimic -deficient mutants were tested heterologous systems. In Xenopus laevis oocytes, S280D, S283D S280D/S283D, had significantly greater ion conductance for Na+ K+, whereas S280A S280A/S283A...