A5090562123- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Protein purification and stability
- Plant Molecular Biology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Plant-Microbe Interactions and Immunity
- Light effects on plants
- Plant Pathogenic Bacteria Studies
- Lipid metabolism and biosynthesis
- Algal biology and biofuel production
- Polysaccharides and Plant Cell Walls
- Plant responses to water stress
- Microbial Metabolic Engineering and Bioproduction
- RNA modifications and cancer
- Photoreceptor and optogenetics research
- Plant Disease Resistance and Genetics
- Protist diversity and phylogeny
- Advanced Cellulose Research Studies
- Seed Germination and Physiology
- Plant tissue culture and regeneration
John Innes Centre
2022-2024
Norwich Research Park
2022-2024
Australian National University
2017-2023
National University
2018-2020
Australian Research Council
2019
ARC Centre of Excellence in Plant Energy Biology
2017
Chloroplast retrograde signaling networks are vital for chloroplast biogenesis, operation, and signaling, including excess light drought stress signaling. To date, has been considered in the context of land plant adaptation, but not regarding origin evolution cascades linking function to stomatal regulation. We show that key elements process, nucleotide phosphatase (SAL1) 3'-phosphoadenosine-5'-phosphate (PAP) metabolism, evolved streptophyte algae-the algal ancestors plants. discover an...
Summary Plasmodesmata are plasma membrane‐lined connections that join plant cells to their neighbours, establishing an intercellular cytoplasmic continuum through which molecules can travel between cells, tissues, and organs. As plasmodesmata connect almost all in plants, molecular traffic carries information resources across a range of scales, but dynamic control plasmodesmal aperture change the possible domains exchange under different conditions. Plasmodesmal is controlled by specialised...
Organelle-nuclear retrograde signaling regulates gene expression, but its roles in specialized cells and integration with hormonal remain enigmatic. Here we show that the SAL1-PAP (3′-phosphoadenosine 5′- phosphate) pathway interacts abscisic acid (ABA) to regulate stomatal closure seed germination Arabidopsis. Genetically or exogenously manipulating PAP bypasses canonical components ABA Insensitive 1 (ABI1) Open Stomata (OST1); priming an alternative restores ABA-responsive ROS bursts, ion...
The plant immune system relies on the perception of molecules that signal presence a microbe threat. This triggers transduction mediates range cellular responses via collection molecular machinery including receptors, small molecules, and enzymes. One response to pathogen is restriction cell-to-cell communication by plasmodesmal closure. We previously found while chitin flg22 trigger specialized signaling cascades in plasma membrane, both execute closure callose synthesis at plasmodesmata....
Chloroplasts act as environmental sensors, enabling rapid plant stress responses through operational retrograde signaling. While these signals operate on minutes-to-hours' time scales, their cumulative impact and function across a plant's lifecycle in field conditions remains unknown. We investigated if signaling's transient changes to gene expression have effects by generating wheat mutants with primed SAL1-PAP pathway responsiveness. After confirming photosynthesis drought resilience under...
Abstract The plant immune system relies on the perception of molecules that signal presence a microbe threat. This triggers transduction mediates range cellular responses via collection molecular machinery including receptors, small molecules, and enzymes. One response to pathogen is restriction cell-to-cell communication by plasmodesmal closure. We previously found while chitin flg22 trigger specialized signaling cascades in plasma membrane, both execute closure callose synthesis at...
Abstract Plant cells are connected to their neighbors via plasmodesmata facilitating the exchange of nutrients and signaling molecules. During immune responses, close, but how this contributes towards a full response is unknown. To investigate this, we developed two transgenic lines with which could induce plasmodesmal closure independently elicitors, using over-active CALLOSE SYNTHASE3 allele icals3m C-terminus PDLP1 drive callose deposition at plasmodesmata. Induction increased expression...
Protocol for recombinant protein expression in E. coli purification and subsequent enzyme assays, crystallography etc.
Abstract Cellular responses to abiotic stress involve multiple signals including secondary messengers such as reactive oxygen species (ROS) and Ca 2+ , phytohormones abscisic acid (ABA) chloroplast-to-nucleus retrograde 3’-phosphoadenosine 5’-phosphate (PAP). Mechanism(s) by which these messengers, produced in different subcellular compartments, intersect for cell regulation remain enigmatic. Previously we showed that the chloroplast signal PAP, similar ABA, induces an increase ROS levels...
Protocol for recombinant protein expression in E. coli enzyme assays, crystallography etc.
Protocol for recombinant protein expression in E. coli purification and subsequent enzyme assays, crystallography etc.
Protocol for recombinant protein expression in E. coli purification subsequent enzyme assays, crystallography etc.
Protocol for recombinant protein expression in E. coli purification and subsequent enzyme assays, crystallography etc.