A5053760133- Celiac Disease Research and Management
- Food composition and properties
- Wheat and Barley Genetics and Pathology
- Phytase and its Applications
- Microscopic Colitis
- Food Allergy and Anaphylaxis Research
- Photosynthetic Processes and Mechanisms
- Enzyme Production and Characterization
- Fermentation and Sensory Analysis
- Microbial Metabolites in Food Biotechnology
- Algal biology and biofuel production
- Genetic Mapping and Diversity in Plants and Animals
- Antioxidant Activity and Oxidative Stress
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Soybean genetics and cultivation
- Genetics and Plant Breeding
- Proteins in Food Systems
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Crop Yield and Soil Fertility
- Advanced Proteomics Techniques and Applications
- Transgenic Plants and Applications
- Microbial Inactivation Methods
- Microbial Community Ecology and Physiology
Commonwealth Scientific and Industrial Research Organisation
2015-2024
Agriculture and Food
2017-2024
ACT Government
2013-2021
Washington State University
2020
Plant Industry
2003-2014
Animal, Food and Health Sciences
2013
Arizona State University
1998-2001
Australian National University
1993-1996
Centre National de la Recherche Scientifique
1996
Measuring grain characteristics is an integral component of cereal breeding and research into genetic control seed development. Measures such as thousand weight are fast, but do not give indication variation within a sample. Other methods exist for detailed analysis size, generally costly very low throughput. Grain colour difficult to perform with accuracy, existing expensive involved.We have developed software method measure size from images captured consumer level flatbed scanners, in...
An increasing world population, rising affluence, urbanization, and changing eating habits are all contributing to the diversification of protein production. Protein is a building block life an essential part healthy diet, providing amino acids for growth repair. The challenges opportunities production protein-rich foods from animals (meat, dairy, aquaculture), plant-based sources (pulses), emerging (insects, yeast, microalgae) discussed against backdrop palatability, nutrition, sustainability.
The suite of prolamin proteins present in barley flour was characterized this study, which we provide spectral evidence for 3 previously prolamins, 8 prolamins with only transcript evidence, and 19 genome-derived predicted prolamins. An additional 9 were identified by searching the complete set against an unannotated translated EST database. Analyses wort, liquid extracted from mashing process during beer production, undertaken a similar identified. We have demonstrated using tandem mass...
Background Subjects suffering from coeliac disease, gluten allergy/intolerance must adopt a lifelong avoidance of gluten. Beer contains trace levels hordeins (gluten) which are too high to be safely consumed by most coeliacs. Accurate measurement ELISA is problematic. Methods We have compared hordein in sixty beers, sandwich ELISA, with the level determined using multiple reaction monitoring mass spectrometry (MRM-MS). Results Hordein measured varied four orders magnitude, zero (for known...
Global proteomic analysis utilizing SDS-PAGE, Western blotting and LC–MS/MS of total protein gluten-enriched extracts derived from 16 economically important cereals was undertaken, providing a foundation for the development MS-based quantitative methodologies that would enable detection wheat contamination in foods. The number proteins identified each grain correlated with entries publicly available databases, highlighting importance continued advances genome sequencing to facilitate...
Coeliac disease is a well-defined condition that estimated to affect approximately 1% of the population worldwide. Noncoeliac gluten sensitivity less well defined, but up 10% population, and often self-diagnosed. At present, only remedy for both conditions lifelong gluten-free diet. A diet expensive, high in fat low fibre, which themselves can lead adverse health outcomes. Thus, there an opportunity use novel plant breeding strategies develop alternative grains. In this work, we describe...
The genome of Synechocystis sp. PCC 6803 contains three sets genes for terminal respiratory oxidases: the previously identified cytochrome aa3-type c oxidase (CtaI), a second putative (CtaII) that we interpret to be bo-type quinol oxidase, and bd (Cyd). Genes two oxidases were cloned, deletion constructs made. Strains lack one, two, or all generated. Deletion had no effect on photoautotrophic photomixotrophic growth. one respire at near-wild-type rates, whereas those both CtaI Cyd do not...
Late maturity α-amylase (LMA) and pre-harvest sprouting (PHS) are both recognized as environmentally induced grain quality defects resulting from abnormally high levels of α-amylase. LMA is a more recently identified issue that now receiving increasing attention worldwide whose prevalence seen impeding the development superior wheat varieties. genetic defect present in specific genotypes characterized by elevated pI TaAMY1 α-amylase, triggered environmental stress during development. remains...
Chloroplasts act as environmental sensors, enabling rapid plant stress responses through operational retrograde signaling. While these signals operate on minutes-to-hours' time scales, their cumulative impact and function across a plant's lifecycle in field conditions remains unknown. We investigated if signaling's transient changes to gene expression have effects by generating wheat mutants with primed SAL1-PAP pathway responsiveness. After confirming photosynthesis drought resilience under...
ABSTRACT The open reading frames sll 1625 and 0823, which have significant sequence similarity to genes coding for the FeS subunits of succinate dehydrogenase fumarate reductase, were deleted singly in combination cyanobacterium Synechocystis sp. strain PCC 6803. When organic acid content Δ 0823 strains was analyzed, a 100-fold decrease concentrations observed relative wild type. A similar analysis revealed that 17% wild-type levels remained, while only 1 2% present. Addition 2-oxoglutarate...
ABSTRACT Analysis of the genome Synechocystis sp. strain PCC 6803 reveals three open reading frames ( slr0851 , slr1743 and sll1484 ) that may code for type 2 NAD(P)H dehydrogenases (NDH-2). The sequence similarity between translated NDH-2s from other organisms is low, generally not exceeding 30% identity. However, flavin adenine dinucleotide binding motifs are conserved in all putative 6803. were cloned, deletion constructs made each. An expression construct containing one frames, was able...
Summary A novel mechanism for increasing vegetative biomass and grain yield has been identified in wheat ( Triticum aestivum ). RNAi‐mediated down‐regulation of Glucan, Water‐Dikinase (GWD), the primary enzyme required starch phosphorylation, under control an endosperm‐specific promoter, resulted a decrease phosphate content increase size. Unexpectedly, consistent increases were observed subsequent generations. In lines where GWD expression was decreased, germination rate slightly reduced....
Background Coeliacs require a life-long gluten-free diet supported by accurate measurement of gluten (hordein) in food. The food industry, with value excess $6 billion 2011, currently depends on two ELISA protocols calibrated against standards that may not be representative the sample being assayed. Aim factors affecting accuracy analysis hordeins beer were examined. Results A simple alcohol-dithiothreitol extraction protocol successfully extracts majority from barley flour and malt. Primary...
Summary Late maturity α‐amylase ( LMA ) and preharvest sprouting PHS are genetic defects in wheat. They both characterized by the expression of specific isoforms particular genotypes grain prior to harvest. The enhanced results a reduction Falling Number (FN), test gel viscosity, subsequent downgrading grain, along with reduced price for growers. FN is unable distinguish between ; thus, treated similarly when traded. However, ‐affected grains, proteases other degradative process activated,...
Wheat starch degradation requires the synergistic action of different amylolytic enzymes. Our spatio-temporal study wheat α-amylases throughout grain development shows that AMY3 is most abundant isoform compared with other known α-amylases. Endosperm-specific over-expression resulted in an increase total α-amylase activity harvested grains. Unexpectedly, increased did not have a significant impact on content or composition but led to soluble carbohydrate (mainly sucrose) dry grain. In...
The efficiency of gluten extraction is critical importance to the results derived from any analytical method for detection and quantitation, whether it employs reagent-based technology (antibodies) or instrumentation (mass spectrometry). If target proteins are not efficiently extracted, end result will be an under-estimation in content posing a health risk people affected by conditions such as celiac disease (CD) nonceliac sensitivity (NCGS). Five different protocols were investigated using...
A crucial step in the evaluation of newly produced transgenic plants is selection homozygous plants. Here we describe an efficient and highly flexible real-time PCR-based method for development lines plant models with complex (multiple) genomes and/or relatively long generation times (>3 months) using direct copy number determinations. An existing DNA extraction was converted into a high-throughput leaf procedure yielding suitable PCR analyses. Highly specific primer pairs were developed...
Celiac disease (CD) is a of the small intestine that occurs in genetically susceptible subjects triggered by ingestion cereal gluten proteins for which only treatment strict adherence to life-long gluten-free diet. Barley contains four protein families, and existence barley genotypes do not accumulate B-, C-, D-hordeins paved way development an ultralow phenotype. Using conventional breeding strategies, three null mutations behaving as recessive alleles were combined create hordein...