A5014421902- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Plant Disease Resistance and Genetics
- Cell Image Analysis Techniques
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- Ubiquitin and proteasome pathways
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
Max Planck Institute for Plant Breeding Research
2025
Cluster of Excellence on Plant Sciences
2025
Norwich Research Park
2019-2024
John Innes Centre
2019-2024
The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition. PM receptors can be dynamically sorted into domains to underpin signaling response extracellular stimuli. In plants, the plasmodesmal a discrete microdomain that hosts specific responses. We exploited independence this domain investigate how independently integrate signal triggers responses across cell. Focusing on chitin signaling, we found require LysM receptor...
Abstract Plasmodesmata are cytosolic bridges, lined by the plasma membrane and traversed endoplasmic reticulum; plasmodesmata connect cells tissues, critical for many aspects of plant biology. While notoriously difficult to extract, tissue fractionation proteomic analyses can yield valuable knowledge their composition. Here we have generated two novel proteomes expand taxonomic representation plasmodesmata: one from mature Arabidopsis leaves moss Physcomitrium patens, leveraged these...
Establishment of the apoplastic root barrier known as Casparian strip occurs early in development. In legumes, this area overlaps with nitrogen-fixing nodule formation, which raises possibility that nodulation and formation are connected. Nodules also contain strips, yet, case, their role is unknown. We established mutants defective barriers Lotus japonicus . This revealed effective blockage endodermis important for root-to-shoot signals underlying nodulation. Our findings further nodules,...
Abstract Colocalisation microscopy analysis provides an intuitive and straightforward way of determining if two biomolecules occupy the same diffraction‐limited volume. A popular colocalisation coefficient, Pearson's correlation coefficient (PCC), can be calculated using different pixel selection criteria: PCC ALL includes all image pixels, OR only pixels exceeding intensity thresholds for either one detection channels, AND both channels. Our results show that depends on foreground to...
Abstract The plasma membrane (PM) that lines plasmodesmata has a distinct protein and lipid composition, underpinning specific regulation of these connections between cells. plasmodesmal PM can integrate extracellular signals differently from the cellular PM, but it is not known how this specificity established or single stimulus trigger independent signalling cascades in neighbouring domains. Here we have used fungal elicitor chitin to investigate signal integration responses at PM. We...
Abstract EXO70s are uniquely expanded in land plants compared to all other eukaryotic lineages. The functional implications of this expansion and diversification on the conserved role EXO70 as a subunit octameric exocyst complex have remained unresolved. We previously demonstrated barley ( Hordeum vulgare ) EXO70FX12, member monocot-specific EXO70FX clade, is required for resistance wheat stripe rust conjunction with leucine-rich repeat receptor kinase (LRR-RK) HvPUR1. Through phylogenetic...
Abstract Plasmodesmata connect neighbouring plant cells across the cell wall. They are cytosolic bridges, lined by plasma membrane and traversed endoplasmic reticulum to these components between tissues. While plasmodesmata notoriously difficult extract, tissue fractionation proteomic analyses have yielded valuable knowledge of their composition. Most profiles originate from suspension cultures in which simple dominate been exclusively generated dicotyledonous species. Here we two novel...