A5015833446- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Plant Virus Research Studies
- Fungal and yeast genetics research
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Biochemical and Structural Characterization
- Mycorrhizal Fungi and Plant Interactions
- Plant biochemistry and biosynthesis
- Advanced Proteomics Techniques and Applications
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
- Studies on Chitinases and Chitosanases
- Fungal Biology and Applications
- Microbial Metabolites in Food Biotechnology
- Transgenic Plants and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Sainsbury Laboratory
2016-2025
Norwich Research Park
2015-2025
University of East Anglia
2018-2025
Utrecht University
2004-2016
Ithaca College
2004-2005
Cornell University
2004
Leiden University
1999-2000
Perception of general elicitors by plant cells initiates signal transduction cascades that are regulated protein phosphorylation. The earliest signaling events occur within minutes and include ion fluxes across the plasma membrane, activation MAPKs, formation reactive oxygen species. phosphorylation regulate these largely unknown. Here we present a mass spectrometry-based quantitative phosphoproteomics approach identified differentially phosphorylated sites in response proteins from...
Plants deploy numerous cell surface-localized pattern-recognition receptors (PRRs) to perceive host- and microbe-derived molecular patterns that are specifically released during infection activate defense responses. The activation of the mitogen-activated protein kinases MPK3, MPK4, MPK6 (MPK3/4/6) is a hallmark immune system by all known PRRs crucial for establishing disease resistance. MAP kinase (MAPKKK) MEKK1 controls MPK4 activation, but MAPKKKs responsible MPK3/6 downstream diverse how...
Plants use autophagy to safeguard against infectious diseases. However, how plant pathogens interfere with autophagy-related processes is unknown. Here, we show that PexRD54, an effector from the Irish potato famine pathogen Phytophthora infestans, binds host protein ATG8CL stimulate autophagosome formation. PexRD54 depletes cargo receptor Joka2 out of complexes and interferes Joka2's positive effect on defense. Thus, a has evolved antagonize counteract defenses.
Innate immunity relies on the perception of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) located host cell's surface. Many plant PRRs are kinases. Here, we report that Arabidopsis receptor kinase EF-TU RECEPTOR (EFR), which perceives elf18 peptide derived from bacterial elongation factor Tu, is activated upon ligand binding phosphorylation its tyrosine residues. Phosphorylation a single residue, Y836, required for activation EFR and downstream to...
Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to detect pathogen effectors and activate local systemic defence. NRG1 ADR1 "helper" NLRs (RNLs) cooperate with enhanced disease susceptibility 1 (EDS1), senescence-associated gene 101 (SAG101) phytoalexin-deficient 4 (PAD4) lipase-like proteins mediate signalling from TIR domain NLR (TNLs). The mechanism of RNL/EDS1 family protein cooperation is not understood. Here, we present genetic molecular...
Two key genes in terpenoid indole alkaloid biosynthesis, Tdc and Str, encoding tryptophan decarboxylase strictosidine synthase, respectively, are coordinately induced by fungal elicitors suspension-cultured Catharanthus roseus cells. We have studied the roles of jasmonate biosynthetic pathway protein phosphorylation signal transduction initiated a partially purified elicitor from yeast extract. In addition to activating Str gene expression, also biosynthesis jasmonic acid. The precursor...
Here, we use a loss-of-function approach to demonstrate that the Arabidopsis (Arabidopsis thaliana) mitogen-activated protein kinase (MAPK) MPK6 plays role in resistance certain pathogens. MPK6-silenced showed no apparent morphological phenotype or reduced fertility, indicating is not required for development. However, resistances an avirulent strain of Peronospora parasitica and virulent strains Pseudomonas syringae were compromised, suggesting both gene–mediated basal resistance....
The salicylic acid-induced protein kinase (SIPK) of tobacco, which is a mitogen-activated (MAPK), activated by various biotic and abiotic treatments. Overexpression SIPK has been shown to trigger cell death. In this study, targeted yeast two-hybrid approach identified the tobacco transcription factor WRKY1 as potential substrate. phosphorylated WRKY1, resulted in enhanced DNA-binding activity its cognate binding site, W box sequence from chitinase gene CHN50. SIPK-mediated enhancement was...
Plants recognize pathogen-associated molecular patterns (PAMPs) via cell surface-localized pattern recognition receptors (PRRs), leading to PRR-triggered immunity (PTI). The Arabidopsis cytoplasmic kinase BIK1 is a downstream substrate of several PRR complexes. How plant PTI negatively regulated not fully understood. Here, we identify the protein phosphatase PP2C38 as negative regulator activity and BIK1-mediated immunity. dynamically associates with BIK1, well PRRs FLS2 EFR, but co-receptor...
Summary Plant immunity consists of two arms: pathogen‐associated molecular pattern (PAMP)‐triggered (PTI), induced by surface‐localized receptors, and effector‐triggered (ETI), intracellular receptors. Despite the little structural similarity, both receptor types activate similar responses with different dynamics. To better understand phosphorylation events during ETI, we employed a phosphoproteomic screen using an inducible expression system bacterial effector avrRpt2 in Arabidopsis...
Magnaporthe oryzae is the causal agent of rice blast disease, most devastating disease cultivated (Oryza sativa) and a continuing threat to global food security. To cause fungus elaborates specialized infection cell called an appressorium, which breaches cuticle leaf, allowing entry plant tissue. Here, we show that exocyst complex localizes tips growing hyphae during vegetative growth, ahead Spitzenkörper, required for polarized exocytosis. However, infection-related development,...
In plants, nucleotide-binding domain and leucine-rich repeat (NLR)-containing proteins can form receptor networks to confer hypersensitive cell death innate immunity. One class of NLRs, known as NLR required for (NRCs), are central nodes in a complex network that protects against multiple pathogens comprises up half the NLRome solanaceous plants. Given prevalence this network, we hypothesised convergently evolved secrete effectors target NRC activities. To test this, screened library 165...
Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically associating with molecules modulating their activities. This study aimed to identify the processes targeted RXLR class of host-translocated potato blight pathogen Phytophthora infestans. To this end, we performed an in planta protein-protein interaction screen transiently expressing P. infestans Nicotiana benthamiana leaves followed coimmunoprecipitation liquid...
Pathogens produce diverse effector proteins to manipulate host cellular processes. However, how functional diversity is generated in an repertoire poorly understood. Many effectors the devastating plant pathogen Phytophthora contain tandem repeats of "(L)WY" motif, which are structurally conserved but variable sequences. Here, we discovered a module formed by specific (L)WY-LWY combination multiple effectors, efficiently recruits serine/threonine protein phosphatase 2A (PP2A) core enzyme...
The rapid production of reactive oxygen species (ROS) is a key signaling output in plant immunity. In the angiosperm model Arabidopsis thaliana (hereafter Arabidopsis), recognition non- or altered-self elicitor patterns by cell-surface immune receptors activates receptor-like cytoplasmic kinases (RLCKs) AVRPPHB SUSCEPTIBLE 1 (PBS1)-like (PBL) family, particularly BOTRYTIS-INDUCED KINASE1 (BIK1).1Lu D. Wu S. Gao X. Zhang Y. Shan L. He P. A kinase, BIK1, associates with flagellin receptor...