A5080745727- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Pathogenic Bacteria Studies
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- 14-3-3 protein interactions
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- Ubiquitin and proteasome pathways
- Plant pathogens and resistance mechanisms
- Plant biochemistry and biosynthesis
- Genomics, phytochemicals, and oxidative stress
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Plant and Fungal Interactions Research
- Seed Germination and Physiology
- Plant Taxonomy and Phylogenetics
- Moringa oleifera research and applications
- Plant Genetic and Mutation Studies
Chungnam National University
2014-2024
University of Illinois Urbana-Champaign
2009-2018
University of California, Berkeley
2018
Sunchon National University
2016
Agricultural Research Service
2009-2014
University of Illinois System
2013
Urbana University
2009-2013
Donald Danforth Plant Science Center
2012
University of Missouri
2012
University of California, Davis
2012
Brassinosteroids (BRs) are essential growth-promoting hormones that regulate many aspects of plant growth and development. Two leucine-rich repeat receptor-like kinases (LRR-RLKs) involved in BR perception signal transduction: brassinosteroid insensitive 1 (BRI1), which is the receptor, its coreceptor BRI1-associated kinase (BAK1). Both proteins classified as serine/threonine protein kinases, but here we report recombinant cytoplasmic domains BRI1 BAK1 also autophosphorylate on tyrosine...
Innate immunity relies on the perception of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) located host cell's surface. Many plant PRRs are kinases. Here, we report that Arabidopsis receptor kinase EF-TU RECEPTOR (EFR), which perceives elf18 peptide derived from bacterial elongation factor Tu, is activated upon ligand binding phosphorylation its tyrosine residues. Phosphorylation a single residue, Y836, required for activation EFR and downstream to...
Abstract Lysine acetylation (LysAc), a form of reversible protein posttranslational modification previously known only for histone regulation in plants, is shown to be widespread Arabidopsis (Arabidopsis thaliana). Sixty-four Lys sites were identified on 57 proteins, which operate wide variety pathways/processes and are located various cellular compartments. A number photosynthesis-related proteins among this group LysAc including photosystem II (PSII) subunits, light-harvesting chlorophyll...
Abstract BRASSINOSTEROID-INSENSITIVE 1 (BRI1) encodes a putative Leucine-rich repeat receptor kinase in Arabidopsis that has been shown by genetic and molecular analysis to be critical component of brassinosteroid signal transduction. In this study we examined some the biochemical properties BRI1 domain (BRI1-KD) vitro, which might important predictors vivo function. Recombinant BRI1-KD autophosphorylated on serine (Ser) threonine (Thr) residues with p-Ser predominating. Matrix-assisted...
BAK1 is a leucine-rich repeat receptor-like kinase that functions as coreceptor with the brassinosteroid (BR) receptor BRI1 and flagellin FLS2, negative regulator of programmed cell death. has been shown to autophosphorylate on numerous serine/threonine sites in vitro well transphosphorylate associated kinases both planta. In present study we identify Tyr-610 carboxyl-terminal domain major site autophosphorylation brassinolide-induced vivo requires kinase-active BAK1. Expression...
The mechanisms involved in sensing oxidative signalling molecules, such as H2O2, plant and animal cells are not completely understood. In the present study, we tested postulate that oxidation of Met (methionine) to MetSO (Met sulfoxide) can couple signals changes protein phosphorylation. We demonstrate when a residue functions hydrophobic recognition element within phosphorylation motif, its strongly inhibit peptide vitro. This is shown occur with recombinant soybean CDPKs (calcium-dependent...
The BRASSINOSTEROID INSENSITIVE1 (BRI1) receptor kinase has recently been shown to possess tyrosine activity, and preventing autophosphorylation of the tyrosine-831 regulatory site by site-directed mutagenesis enhances shoot growth. In this study, we characterized increased leaf growth Arabidopsis (Arabidopsis thaliana) plants expressing BRI1(Y831F)-Flag compared with BRI1-Flag (both driven native promoter expressed in bri1-5 weak allele background) provide insights into possible mechanisms...
The activity of the dual-specificity receptor kinase, brassinosteroid insensitive 1 (BRI1), reflects balance between phosphorylation-dependent activation and several potential mechanisms for deactivation receptor. In present report, we elucidate a unique mechanism that involves autophosphorylation serine-891 in ATP-binding domain. Serine-891 was identified previously as site by mass spectrometry, sequence-specific antibodies mutagenesis studies now unambiguously establish phosphorylation...
The receptor kinase BRI1 (BRASSINOSTEROID-INSENSITIVE 1) is a key component in BR (brassinosteroid) perception and signal transduction, has broad impact on plant growth development. In the present study, we demonstrate that Arabidopsis CaM (calmodulin) binds to recombinant cytoplasmic domain of Ca2+-dependent manner vitro. silico analysis predicted binding Helix E subdomain VIa synthetic peptide based this sequence interacted with Ca2+/CaM. Co-expression Escherichia coli strongly reduced...
Among several receptor-like kinases (RLKs), leucine-rich-repeat (LRR-RLKs) are a major group of genes that play crucial roles in growth, development and stress responses plant systems. Given they have functional roles, it is important to investigate their Brassica rapa. In the present study, 303 LRR-RLKs were identified genome B. rapa comparative phylogenetic analysis 1213 combined rapa, Arabidopsis thaliana, Oryza sativa Populus trichocarpa helped us categorize gene family into 15...
Brassinosteroids promote soybean epicotyl elongation and regulate expression of BRU1 a gene with homology to xyloglucan endotransglycosylases (XETs). Recombinant protein possesses XET activity in situ hybridization reveals highest transcript accumulation inner tissue, particularly the phloem paratracheary parenchyma cells. These results suggest role for vascular development addition cell elongation.
Abstract The Arabidopsis TCH4 gene is up-regulated in expression by diverse environmental and hormonal stimuli. BecauseTCH4 encodes a xyloglucan endotransglucosylase/hydrolase, this change may reflect recruitment of cell wall-modifying activity response to stress growth. How stimuli lead the common regulation not known. Here, we show that induction touch, darkness, cold, heat, brassinosteroids (BRs) conferred reporter genes same 102-bp 5′-untranscribed TCH4region; result consistent with idea...
Transgenic plants overexpressing AXR3/IAA17 were impaired in root growth. Specifically, they exhibited severe defects lateral and hair development similar to the phenotypes of epi-brassinolide (epiBL)-treated wild-type plants. Here, we investigated involvement gene expression brassinosteroid (BR)-regulated development. Exogenous epiBL application significantly induced as well several Aux/IAA genes, such AXR2/IAA7, SLR/IAA14, IAA28. We analyzed transcription levels genes related BR signaling...
In metazoans, receptor kinases control many essential processes related to growth and development response the environment. The in plants animals are structurally similar but evolutionarily distinct thus while most animal tyrosine plant classified as serine/threonine kinases. One of best studied is Brassinosteroid Insensitive 1 (BRI1), which functions brassinosteroid signaling. Consistent with its classification, BRI1 was shown early studies autophosphorylate vitro exclusively on serine...
The soybean (Glycine max) genome contains 18 members of the 14-3-3 protein family, but little is known about their association with specific phenotypes. Here, we report that Glyma0529080Soybean G-box Factor 14-3-3c (SGF14c) and Glyma08g12220 (SGF14l) genes, encoding proteins, appear to play essential roles in nodulation. Quantitative reverse transcription-polymerase chain reaction western-immunoblot analyses showed SGF14c mRNA levels were specifically increased abundance nodulated roots 10,...
Reddish purple Chinese cabbage (RPCC) is a popular variety of Brassica rapa (AA = 20). It rich in anthocyanins, which have many health benefits. We detected novel anthocyanins including cyanidin 3-(feruloyl) diglucoside-5-(malonoyl) glucoside and pelargonidin 3-(caffeoyl) RPCC. Analyses transcriptome data revealed 32,395 genes 3345 differentially expressed (DEGs) between 3-week-old RPCC green (GCC). The DEGs included 218 transcription factor (TF) some functionally uncharacterized genes....
GmCDPKβ phosphorylates by protein kinase assay (View interaction)
Abstract Plasmodesmata (PDs) are intercellular organelles carrying multiple membranous nanochannels that allow the trafficking of cellular signalling molecules. The channel regulation PDs occurs dynamically and is required in various developmental physiological processes. It well known callose a critical component regulating PD permeability or symplasmic connectivity, but understanding pathways mechanisms its limited. Here, we used reverse genetic approach to investigate role C‐type lectin...
Plants must constantly adjust their growth and defense responses to deal with the wide variety of stresses they encounter in environment. Among phytohormones, brassinosteroids (BRs) are an important group plant steroid hormones involved numerous aspects lifecycle including growth, development various insect attacks. Here, we show that BRs regulate glucosinolate (GS) biosynthesis function herbivory. Preference tests larval feeding experiments using generalist herbivore, diamondback moth (...
This article has been retracted
Protein kinase specificity is of fundamental importance to pathway regulation and signal transduction. Here, we report a convenient system monitor the activity recombinant protein kinases expressed in E. coli. We apply this study cytoplasmic domain plant receptor BRASSINOSTEROID-INSENSITIVE 1 (BRI1), which functions brassinosteroid (BR) signaling. Recombinant BRI1 catalytically active both autophosphorylates transphosphorylates coli proteins situ. Using enrichment approaches followed by...