A5056345876- Plant Molecular Biology Research
- Nitrogen and Sulfur Effects on Brassica
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Phytase and its Applications
- Legume Nitrogen Fixing Symbiosis
- Soybean genetics and cultivation
- Photosynthetic Processes and Mechanisms
- Cancer therapeutics and mechanisms
- Lipid metabolism and biosynthesis
- Plant Gene Expression Analysis
- Microbial Metabolites in Food Biotechnology
- Plant Stress Responses and Tolerance
- Glycosylation and Glycoproteins Research
- DNA Repair Mechanisms
- Plant-Microbe Interactions and Immunity
- Enzyme Production and Characterization
- Nutrition, Genetics, and Disease
- Seed Germination and Physiology
- Plant Micronutrient Interactions and Effects
- Calcium signaling and nucleotide metabolism
- Chromosomal and Genetic Variations
- Phytoestrogen effects and research
- Polyomavirus and related diseases
- DNA and Nucleic Acid Chemistry
Seoul National University
2003-2021
Mokpo National University
2010
The University of Tokyo
1997-2003
Abstract Vernalization, a long-term cold-mediated acquisition of flowering competence, is critically regulated by VERNALIZATION INSENSITIVE 3 (VIN3), gene induced vernalization in Arabidopsis. Although the function VIN3 has been extensively studied, how expression itself upregulated cold not well understood. In this study, we identified vernalization-responsive cis-element promoter, VREVIN3, composed G-box and an evening element (EE). Mutations either or EE prevented from being fully upon...
Transgenic plants overexpressing AXR3/IAA17 were impaired in root growth. Specifically, they exhibited severe defects lateral and hair development similar to the phenotypes of epi-brassinolide (epiBL)-treated wild-type plants. Here, we investigated involvement gene expression brassinosteroid (BR)-regulated development. Exogenous epiBL application significantly induced as well several Aux/IAA genes, such AXR2/IAA7, SLR/IAA14, IAA28. We analyzed transcription levels genes related BR signaling...
TATA-binding protein-associated factors (TAFs) are general transcription within the factor IID (TFIID) complex, which recognizes core promoter of genes. In addition to their biochemical function, it is known that several TAFs involved in regulation developmental processes. this study, we found TAF15b affects flowering time, especially through autonomous pathway (AP) Arabidopsis. The mutant taf15b shows late compared with wild type plant during both long and short days, vernalization...
The first intron of the petunia actin-depolymerizing factor 1 (PhADF1) gene was previously shown to induce strong and constitutive expression that in vegetative tissues transgenic Arabidopsis. To examine intron-mediated enhancement PhADF1 detail, effects splicing, deletion promoter alteration on were analyzed this study. Deletion 5' upstream region significantly reduced level enhancement, under control both PhADF2 promoters. ratio pre-mRNA mRNA does not correlate with enhancement. determine...
Summary Kinetochore, a protein super‐complex on the centromere of chromosomes, mediates chromosome segregation during cell division by providing attachment sites for spindle microtubules. The NDC 80 complex, composed four proteins, 80, NUF 2, SPC 24 and 25, is localized at outer kinetochore connects fibers to kinetochore. Although it conserved across species, functional studies this complex are rare in Arabidopsis. Here, we characterize recessive mutant, meristem unstructured‐1 ( mun‐1 ),...
Abstract Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is a key enzyme that hydrolyzes the phosphodiester bond between tyrosine of topoisomerase and 3′-phosphate DNA repairs topoisomerase-mediated damage during chromosome metabolism. However, functional Tdp1 has only been described in yeast human to date. In human, mutations gene are involved disease spinocerebellar ataxia with axonal neuropathy. plants, we have identified nuclear protein AtTDP, homolog from Arabidopsis (Arabidopsis thaliana). The...
TDP1 (tyrosyl-DNA phosphodiesterase 1), a member of the PLD (phospholipase D) superfamily, catalyses hydrolysis phosphodiester bond between tyrosine residue and 3'-phosphate DNA. We have previously identified characterized AtTDP gene in Arabidopsis thaliana, an orthologue yeast human genes. Sequence alignment orthologues revealed that has both conserved C-terminal TDP domain and, uniquely, N-terminal SMAD/FHA (forkhead-associated) domain. To help understand function this novel enzyme, we...
The application of glutathione to immature soybean cotyledons reduced the accumulation β subunit β-conglycinin, and increased most glycinins. Both oxidized forms had these effects. an inhibitor synthesis, buthionine sulfoximine, subunit. These results suggest that is important in affecting composition seed storage proteins.
Abstract The gene encoding the β subunit of β-conglycinin, one major seed storage proteins soybean (Glycine max [L.] Merr.) , is downregulated by methionine (Met) and upregulated O-acetyl-l-serine (OAS). We examined interaction between Met OAS in regulation expression vitro-cultured immature cotyledons. Application reduced accumulation both presence absence media: increased Met. also studied promoter sulfur Arabidopsis thaliana (L.) Heynh. mutant mto1-1, that over-accumulates soluble Met,...
In Arabidopsis, inflorescence stem formation is a critical process in phase transition from the vegetative to reproductive state. Although development has been reported depend on expression of variety genes during floral induction and repression, little known about molecular mechanisms involved control formation. By activation T-DNA tagging mutagenesis dominant gain-of-function mutation, eve1-D (eternally phase1-Dominant), which lost ability form an stem, was isolated. The mutation exhibited...
Composition of seed storage proteins in vitro cultured cotyledons is known to be affected by exogenous application abscisic acid (ABA) and sulfur deficiency. In this paper, we analyzed effects ABA on regulation soybean protein accumulation relation that When was applied at 10 μM 100 μM, the β subunit β-conglycinin, a major protein, increased, whereas glycinin, another decreased. Free sulfate concentrations were not significantly ABA. but doubled As have previously shown O-acetyl-L-serine...
sAccumulation of the β subunit β-conglycinin, a major seed storage protein soybean, is known to be upregulated by sulfate deficiency and repressed exogenous application methionine in vitro culture immature cotyledons. Accumulation mRNA increased seeds soybean plants as concentration media was decreased. The promoter capable upregulation, suggesting that regulation mainly occurs at level transcription. free grown under sulfur dramatically reduced. Immature cultured deficient medium...
Abstract Eight independent transgenic lines of Arabidopsis thaliana were generated carrying homozygous insertions the cauliflower mosaic virus 35S RNA promoter driving antisense MT2a ORF or heat shock-protein 81-1 sense ORF. Six showed lower levels mRNA accumulation than wild type plants and among them, three produced abnormal tissue masses at base floral stalks low frequencies. Such structures had never been observed in with transcript plants. Moreover, we isolated a line from ethyl methane...