A5021425965- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Plant Molecular Biology Research
- Higher Education and Teaching Methods
- Ideological and Political Education
- Gastrointestinal motility and disorders
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- SARS-CoV-2 and COVID-19 Research
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Immunotherapy and Immune Responses
- Inflammatory Bowel Disease
- Antimicrobial Resistance in Staphylococcus
- Immune Cell Function and Interaction
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Education and Work Dynamics
- Ubiquitin and proteasome pathways
- Liver physiology and pathology
- Pneumonia and Respiratory Infections
- Cystic Fibrosis Research Advances
- Hepatitis B Virus Studies
- Plant Reproductive Biology
- Bacterial biofilms and quorum sensing
Second Affiliated Hospital of Nanjing Medical University
2019-2025
Guangdong Medical College
2023-2025
Marine Biomedical Research Institute of Qingdao
2025
Jinan University
2020-2024
Nanjing Medical University
2018-2024
Children's Hospital of Fudan University
2013-2024
University of Washington
2013-2023
University of Kentucky
2015-2023
Hunan University
2023
Southwest Medical University
2021-2023
Rationale: Previous work indicates that ivacaftor improves cystic fibrosis transmembrane conductance regulator (CFTR) activity and lung function in people with G551D-CFTR mutations but does not reduce density of bacteria or markers inflammation the airway. These findings raise possibility infection may progress independently CFTR once disease is established.Objectives: To better understand relationship between activity, airway microbiology inflammation, subjects chronic infections.Methods:...
Abstract Lysine acetylation (LysAc), a form of reversible protein posttranslational modification previously known only for histone regulation in plants, is shown to be widespread Arabidopsis (Arabidopsis thaliana). Sixty-four Lys sites were identified on 57 proteins, which operate wide variety pathways/processes and are located various cellular compartments. A number photosynthesis-related proteins among this group LysAc including photosystem II (PSII) subunits, light-harvesting chlorophyll...
Abstract The nosocomial pathogen Acinetobacter baumannii is a frequent cause of hospital-acquired infections worldwide and challenge for treatment due to its evolved resistance antibiotics, including carbapenems. Here, gain insight on A. antibiotic mechanisms, we analyse the protein interaction network multidrug-resistant clinical strain (AB5075). Using in vivo chemical cross-linking mass spectrometry, identify 2,068 non-redundant cross-linked peptide pairs containing 245 intra- 398...
BAK1 is a leucine-rich repeat receptor-like kinase that functions as coreceptor with the brassinosteroid (BR) receptor BRI1 and flagellin FLS2, negative regulator of programmed cell death. has been shown to autophosphorylate on numerous serine/threonine sites in vitro well transphosphorylate associated kinases both planta. In present study we identify Tyr-610 carboxyl-terminal domain major site autophosphorylation brassinolide-induced vivo requires kinase-active BAK1. Expression...
MicroRNAs (miRNAs) are small non-coding RNAs that can posttranscriptionally regulate gene expression by targeting messenger RNAs. During miRNA biogenesis, the star strand (miRNA*) is generally degraded to a low level in cells. However, certain miRNA* express abundantly and be recruited into silencing complex expression. Most miRNAs function as suppressive regulators on Group B coxsackieviruses (CVB) major pathogens of human viral myocarditis dilated cardiomyopathy. CVB genome positive-sense,...
Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression P. roots and leaves. We isolated 2022 bp promoter fragment upstream the translational start from euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased root, leaf, stem tissues salt stress. DNA affinity...
The efficacy of faecal microbiota transplantation (FMT) in Crohn's disease (CD) remains unclear due to lack data. This study aimed assess the value FMT treating CD-related clinical targets. use for CD as a registered trial (NCT01793831) was performed between October 2012 and December 2017. Seven therapeutic targets included abdominal pain, diarrhoea, hematochezia, fever, steroid-dependence, enterocutaneous fistula active perianal fistula. Each target recorded 1 (yes) or 0 (no) during...
Large-scale chemical cross-linking with mass spectrometry (XL-MS) analyses are quickly becoming a powerful means for high-throughput determination of protein structural information and protein-protein interactions. Recent studies have garnered thousands cross-linked interactions, yet the field lacks an effective tool to compile experimental data or access network knowledge these large scale analyses. We present XLinkDB 2.0 which integrates tools analysis, Protein Databank queries, modeling...
The receptor kinase BRI1 (BRASSINOSTEROID-INSENSITIVE 1) is a key component in BR (brassinosteroid) perception and signal transduction, has broad impact on plant growth development. In the present study, we demonstrate that Arabidopsis CaM (calmodulin) binds to recombinant cytoplasmic domain of Ca2+-dependent manner vitro. silico analysis predicted binding Helix E subdomain VIa synthetic peptide based this sequence interacted with Ca2+/CaM. Co-expression Escherichia coli strongly reduced...
Chemical cross-linking mass spectrometry (XL-MS) provides protein structural information by identifying covalently linked proximal amino acid residues on surfaces. The gained this technique is complementary to other biology methods such as x-ray crystallography, NMR and cryo-electron microscopy[1]. extension of traditional quantitative proteomics with chemical can provide the dynamics structures complexes. identification quantitation cross-linked peptides remains challenging for general...
HD561, which was designed to enhance nerve growth, re-engineered into HD56, a carboxylic acid ester prodrug. The goal of this study compare the druggability, species differences, and correlation between in vitro vivo transformation HD56 HD561 from pharmacokinetic (PK) perspective, offering scientific basis for HD56's clinical research. bidirectional transmembrane transport investigated using Caco-2 cells LLC-PK1 overexpressing MDR1 monolayer cells. Recombinant enzymes chemical inhibition...
B-phycoerythrin (B-PE), a pigment protein, has found extensive applications in the food, pharmaceutical, and cosmetic industries. However, effects potential mechanisms of B-PE on colitis colitis-associated bone loss remain unclear. Thus, aim this study was to investigate pharmacological against using network pharmacology analysis, molecular docking, experimental validation. Based public databases, 99 common targets inflammatory bowel disease osteoporosis were predicted. The protein-protein...
As large-scale cross-linking data becomes available, new software tools for processing and visualization are required to replace manual analysis. XLink-DB serves as a storage site tool results. accepts generated with any cross-linker stores them in relational database. Cross-linked sites automatically mapped onto PDB structures if results compared existing protein interaction databases. A network is also the entire set. The server, including examples, help page available noncommercial use at...